Marine Biology

, Volume 147, Issue 3, pp 755–760 | Cite as

In vitro reproduction of the turbellarian Urastoma cyprinae isolated from Mytilus galloprovincialis

  • C. Crespo González
  • R. M. Reza Álvarez
  • H. Rodríguez Domínguez
  • M. Soto Búa
  • R. Iglesias
  • C. Arias Fernández
  • J. M. García Estévez
Research Article

Abstract

Urastoma cyprinae is a species infecting the gills of several marine bivalves. Although there is some literature on this turbellarian, its life cycle remains unknown. In our work we have demonstrated that reproduction of U. cyprinae can be completed out of its host. More than 50% of turbellarians isolated from mussels (Mytilus galloprovincialis) secreted and cemented a cocoon to the well bottom during the first 72 h of incubation in seawater (34‰ salinity) at 14°C. Oviposition started at days 1–18 (average 4.8 days) and occurred in most cases inside the protective cocoon. Each Urastoma laid an average of 2.9 egg capsules (range 1–10) and 3.9 embryos were developed inside each egg capsule (range 1–11). Hatching started at days 20–43 (average 24 days). An average of 12.8 juvenile forms (range 1–64) escaped from the cocoon after hatching. The free-swimming juveniles showed a positive phototactic response and survived about a month after hatching. On the basis of our results, we propose a life cycle for U. cyprinae involving a sexual maturation parasitic period in the bivalve gills and a reproduction period including cocoon secretion, egg laying, and hatching that is entirely completed in the external environment.

Notes

Acknowledgements

This work was supported by grants C2248122P6102 (Universidad de Vigo) and PGIDIT04RMA312002PR (Xunta de Galicia).

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • C. Crespo González
    • 1
  • R. M. Reza Álvarez
    • 1
  • H. Rodríguez Domínguez
    • 1
  • M. Soto Búa
    • 1
  • R. Iglesias
    • 1
  • C. Arias Fernández
    • 1
  • J. M. García Estévez
    • 1
  1. 1.Laboratorio de Parasitología Marina, Facultad de Ciencias del Mar, Edificio de Ciencias ExperimentalesUniversidad de VigoVigoSpain

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