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Marine Biology

, Volume 147, Issue 3, pp 747–754 | Cite as

Genetic and color morph differentiation in the Caribbean sea anemone Condylactis gigantea

  • Nina Stoletzki
  • Bernd Schierwater
Research Article

Abstract

The distribution of phenotypic and genetic variation across environments can provide insights into local adaptation. The tropical sea anemone Condylactis gigantea inhabits a broad spectrum of coral-reef habitats and displays a variety of phenotypes, particularly with respect to color. At the coast of Discovery Bay, Jamaica, individuals with either pink or green tentacle tips show distinct distributions. Pink morphs are more abundant in the lagoon and in deeper areas, while green morphs are more abundant in the forereef and in shallower areas. We use DNA sequence data (ITS1-5.8S) to investigate if variation in color is associated with genetic differentiation in lagoon and forereef habitats about 5 km apart. Population genetic analyses reveal two distinct ITS1-5.8S variants, which differ in relative frequency. The two variants are present in both habitats, but a dearth of intermediates suggests reduced gene flow. In the lagoon, but not the forereef, ITS variants show an association with color. In order to address the potential ecological significance of color, we study UV absorbance and UV acclimatization capacities of pink and green color morphs in the lagoon. Color morphs differed significantly in UV-B absorbance. These results suggest genetic and ecological differentiation in the face of gene flow over short distances.

Keywords

High Performance Liquid Chromatography Gene Flow Forereef Concerted Evolution Color Morph 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

Very many thanks are due to Daven Presgraves who kindly helped to improve the manuscript, for advice and many helpful comments and discussions. We are also very grateful for discussions with Allen Collins, and for many helpful comments of A. Collins, J. Hermisson and B. Nuernberger on earlier versions of the manuscript, and of two anonymous reviewers on the final version. Thanks are due to each of the East/West students helping in the field, the staff members at Discovery Bay Marine Laboratories, Jamaica, and the East/West Marine Biology Program of Northeastern University, Boston, and all the members of the ITZ. Many thanks are also due to Ken Sebens for early discussions. U. Karsten kindly helped with the MAA analyses and allowed the use of his facilities. We acknowledge financial support from the German Science Foundation (DFG Schi-277/10-2) to B. Schierwater and scholarship from the German Academic Exchange Service (DAAD) to N. Stoletzki.

References

  1. Ayre DJ, Hughes TP (2000) Genotypic diversity and gene flow in brooding and spawning corals along the Great Barrier Reef, Australia. Evolution 54:1590–1605PubMedGoogle Scholar
  2. Baldwin BG, Sanderson MJ, Porter JM, Wojciechowski MF, Ulrike Campbell CS, Donoghue MJ (1995) The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Mo Bot Gard 82:247–277Google Scholar
  3. Banaszak AT, Iglesias-Prieto R, Trench RK (1993) Scripsiella velella sp. nov. (Peridinales) and Gloeodinium viscum sp. nov., dinoflagellate symbionts of two hydrozoans (Cnidaria). J Phycol 29:517–528Google Scholar
  4. Benzie JAH (1994) Patterns of gene flow in the Great Barrier Reef and Coral See. In: Beaumont AR (ed) Genetics and evolution of aquatic organisms.Google Scholar
  5. Bohonak AJ (1999) Dispersal, gene flow and population structure. Q Rev Biol 74:21–45CrossRefPubMedGoogle Scholar
  6. Brown BE (1997) Adaptations of reef corals to physical environmental stress. Advances in Marine Biology, vol 31. Academic, New YorkGoogle Scholar
  7. Bruno JF, Edmunds PJ (1997) Clonal variation for phenotypic plasticity in the coral Madracis mirabilis. Ecology 78:2177–2190Google Scholar
  8. Carlon DB, Budd AF (2002) Incipient speciation across a depth gradient in a scleractinian coral. Evolution 56:2227–2242PubMedGoogle Scholar
  9. D’Elia CF, Webb KL, Porter JW (1981) Nitrate-rich groundwater inputs to Discovery Bay, Jamaica: a significant source of N to local coral reefs? Bull Mar Sci 31:903–910Google Scholar
  10. Dove SG, Takabayashi M, Hoegh-Guldberg O (1995) Isolation and partial characterization of the pink and blue pigments of pocilloporid and acroporid corals. Biol Bull 189:288–297Google Scholar
  11. Dove SG, Hoegh-Guldberg O, Ranganathan S (2001) Major colour patterns of reef-building corals are due to a family of GFP-like proteins. Coral Reefs 19:197–204Google Scholar
  12. Dunlap WC, Shick JM (1998) Ultraviolet radiation-absorbing mycosporine-like amino acids in coral reef organisms: a biochemical and environmental perspective. J Phycol 34:418–430CrossRefGoogle Scholar
  13. Endler JA (1977) Geographic variation, speciation, and clines. Princeton University Press, PrincetonGoogle Scholar
  14. Fleischmann EM (1989) The measurement and penetration of ultraviolet radiation into tropical marine water. Limnol Oceanogr 34:1623–1629Google Scholar
  15. Gayle PMH, Woodley JD (1998) Discovery Bay, Jamaica. In: Kjerfve B (ed) CARICOMP Caribbean coral reef, seagrass and mangrove sites. Coastal region and small island papers 3. UNESCO, ParisGoogle Scholar
  16. Gleason DF (1993) Differential effects of ultraviolet radiation on green and brown morphs of the Caribbean coral Porites asteroides. Limnol Oceanogr 38:1452–1463Google Scholar
  17. Gleason DF (1998) Sedimentation and distribution of green and brown morphs of the Caribbean coral Porites asteroides Lamarck. J Exp Mar Biol Ecol 230:73–89CrossRefGoogle Scholar
  18. Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, MartynovVI, Yanushevich YG, Lukyanov KA, Lukyanov SA (2001) GFP-like proteins as a source of far-red fluorescent proteins (1). FEBS Lett 507:16–20CrossRefPubMedGoogle Scholar
  19. Hoyer K, Karsten U, Wiencke C (2002) Induction of sunscreen compounds in Antarctic macroalgae by different radiation conditions. Mar Biol 41:619–627Google Scholar
  20. Hughes TP (1989) A functional biology of clonal animals. Chapman and Hall, LondonGoogle Scholar
  21. Humann P (1992) Reef creatures. New World, Jacksonville, FlaGoogle Scholar
  22. Kelmanson IV, Matz MV (2003) Molecular basis and evolutionary origins of color diversity in great star coral Montastrea cavernosa (Scleractinia: Faviidae).Google Scholar
  23. Liston A, Robinson WA, Oliphant JM, Alvarez-Buylla ER (1996) Length variation in the nuclear ribosomal DNA internal transcribed spacer of non-flowering seed plants. Syst Bot 21:109–120Google Scholar
  24. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov KA (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nature Biotechnol 17:969–973CrossRefGoogle Scholar
  25. Modrich P, Lahue R (1996) Mismatch repair in replication fidelity, genetic recombination, and cancer biology. Annu Rev Biochem 65:101–133CrossRefPubMedGoogle Scholar
  26. Nabeyama M, Kubota S, Kohno S (2000) Concerted evolution of a highly repetitive DNA family in Eptatretidae (Cyclostomata, Agnatha) implies specifically differential homogenization and amplification events in their germ cells. J Mol Evol 50:154–169PubMedGoogle Scholar
  27. Nagylaki T, Petes TD (1982) Intrachromosomal gene conversion and the maintenance of sequence homogeneity among repeated genes. Genetics 100:315–337PubMedGoogle Scholar
  28. Odorico DM, Miller DJ (1997) Variation in the ribosomal internal transcribed spacers and 5.8S rDNA among five species of Acropora (Cnidaria; Scleractinia): patterns of variation consistent with reticulate evolution. Mol Biol Evol 14:465–473PubMedGoogle Scholar
  29. Palumbi SR (1992) Marine speciation on a small planet. Trends Ecol Evol 7:114–118CrossRefGoogle Scholar
  30. Palumbi SR (1994) Genetic divergence, reproductive isolation, and marine speciation. Annu Rev Ecol Syst 25:547–572CrossRefGoogle Scholar
  31. Palumbi SR (1998) Species formation and the evolution of gamete recognition loci. Endless forms: species and speciation. Oxford University Press, Oxford, pp 271–278Google Scholar
  32. Quijada A, Liston A, Robinson W, Alvarez-Buylla E (1997) The ribosomal ITS region as a marker to detect hybridization in pines. Mol Ecol 6:995–996CrossRefGoogle Scholar
  33. Richmond RH, Jokiel PL (1984) Lunar periodicity in larva release in the reef coral Pocillopora damicornis at Enewatak and Hawaii. Bull Mar Sci 34:280–287Google Scholar
  34. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  35. Schluter D (2000) The ecology of adaptive radiation. Oxford University Press, OxfordGoogle Scholar
  36. Schneider S, Roessli D, Excoffier L (2000) Arlequin Version 2000: a software for population genetics data analysis. Genetics and Biometry Laboratory, University of GenevaGoogle Scholar
  37. Shick JM (1991) A functional biology of sea anemones. Chapman and Hall, LondonGoogle Scholar
  38. Shick JM, Dunlap WC (2002) Mycosporine-like amino acids and related gradusols: biosynthesis, accumulation, and UV-protective functions in aquatic organisms. Annu Rev Physiol 64:223–262CrossRefPubMedGoogle Scholar
  39. Shick JM, Dunlap WC, Chalker BE, Banaszak AT, Rosenzweig TK (1992) Survey of ultraviolet radiation-absorbing mycosporine-like amino acids in organs of coral reef holothuroids. Mar Ecol Prog Ser 90:139–148Google Scholar
  40. Slatkin M (1973) Gene flow and selection in a cline. Genetics 75:733–756PubMedGoogle Scholar
  41. Swofford DL (2000) PAUP*: Phylogenetic Analyses Using Parsimony (*and other Methods). Sinauer, Sunderland, MassGoogle Scholar
  42. Takabayashi M, Hoegh-Guldberg O (1995) Ecological and physiological differences between two colour morphs of the coral Pocillopora damicornis. Mar Biol 123:705–714CrossRefGoogle Scholar
  43. Van Oppen MJH, Willis BL, Van Vugt HWJA, Miller DJ (2000) Examination of species boundaries in the Acropora cervicornis group (Scleractinia, Cnidaria) using nuclear DNA sequence analyses. Mol Ecol 9:1363–1373PubMedGoogle Scholar
  44. Van Oppen MJH, Woerheide G, Takabayashi M (2002a) Nuclear markers in evolutionary and population genetic studies of scleractinian corals and sponges. Proc 9th Int Coral Reef Symp, BaliGoogle Scholar
  45. Van Oppen MJH, Willis BL, VanRheede T, Miller D (2002b) Spawning times, reproductive compatibilities and genetic structure in the Acropora aspera group: evidence for natural hybridization and semi-permeable species boundaries in corals. Mol Ecol 11:1363–1376PubMedGoogle Scholar
  46. Vogler AP, DeSalle R (1994) Evolution and phylogenetic information content of the ITS-1 region in the tiger beetle Cicindela dorsalis. Mol Biol Evol 11:393–405PubMedGoogle Scholar
  47. Whitehead RF, de Mora SJ, Demers S (2000) Enhanced UV radiation—a new problem for the marine environment. In: de Mora S, Demers S (eds) The effects of UV radiation in the marine environment. Cambridge University Press, CambridgeGoogle Scholar
  48. Wiedenmann J (2000) The identification of new proteins homologous to GFP from Aequorea victoria as coloring compounds in the morphs of Anemonia sulcata and their biological function. PhD Thesis, University of UlmGoogle Scholar
  49. Williams ST, Benzie JAH (1998) Evidence of a biogeographic break between populations of a high-dispersal starfish: congruent regions within the Indo-West Pacific defined by colour morphs, mtDNA, and allozyme data. Evolution 52:87–99Google Scholar
  50. Woodley JD, Chornesky EA, Clifford PA, Jackson JBC, Kaufman LS, Knowlton N, Lang JC, Pearson MP, Porter JW, Rooney MC, Rylaarsdam KW, Tunnicliffe VJ, Wahle CM, Wulff JL, Curtis ASG, Dallmeyer MD, Jupp BP, Koehl MAR, Neigel J, Sides EM (1981) Hurricane Allen’s impact on Jamaican coral reefs. Science 214:749–755Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.ITZ, Ecology and EvolutionTieraerztliche Hochschule HannoverHannoverGermany
  2. 2.Ludwig-Maximilian UniversitaetPlanegg-MartinsriedGermany

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