Noncovalent bonds are key mechanisms for the cohesion of barnacle (Balanus crenatus) adhesive proteins
Cement of the barnacle species, Balanus crenatus, was isolated and separated by SDS-PAGE. Reductive and nonreductive conditions yielded the same result of six major proteins (in the range up to 100 kDa) occurring as two triplets. A third triplet of lower molecular mass was faintly visible. The presence of a reductive agent had only little impact on the solubility of B. crenatus cement. The strong effect of the denaturing conditions (the presence of SDS and the incubation at elevated temperature) on the solubility of the barnacle cement and the absence of disulfide signals in Raman spectra suggest a complex structure of the barnacle cement noncovalently bound. It can be speculated that this protein complex in its quaternary structure is formed by small subunits and is equivalent to the globular cement structures previously detected.
KeywordsSodium Dodecyl Sulphate PDMS Nonreducing Condition Noncovalent Bond Amino Acid Cysteine
This work is part of a Ph.D. thesis, which was financially supported by the scholarship program of the German Federal Environmental Foundation and by the Fraunhofer Institute for Production Technology and Applied Materials Research—IFAM, Bremen. The support of the laboratory of general microbiology of Prof. Reinhold-Hurek (University Bremen) while conducting the purification and fractioning of the samples is greatly appreciated.
- Abbott A (1990) Bioadhesives: potential for exploitation. Sci Prog 74:131–146Google Scholar
- Lindner E, Dooley C A (1973) Chemical bonding in cirriped adhesive. In: Acker RF (ed) Proceedings of the third international congress on marine corrosion and fouling. National Bureau of Standards, Gaithersburg, Maryland, pp 653–673Google Scholar
- Lin-Vien D, Colthup NB, Fately WG, Grasselli JG (1991) Infrared and Raman characteristic of frequencies of organic molecules. Academic, New YorkGoogle Scholar
- Yan W, Pan S (1981) The solubilising effect of denaturation chemicals on cement of Balanus reticulatus Utinomi. Oceanol Limnol Sin 12:125–132Google Scholar
- Yan W, Tang Y (1981) The biochemical composition of the secondary cement of Balanus reticulatus Utinomi and Balanus amaryllis Darwin. Nanhai Stud Mar Sin 2:145–152Google Scholar