Advertisement

Marine Biology

, Volume 149, Issue 2, pp 257–267 | Cite as

Evidence of a 2-day periodicity of striae formation in the tropical scallop Comptopallium radula using calcein marking

  • Julien Thébault
  • Laurent Chauvaud
  • Jacques Clavier
  • Renaud Fichez
  • Eric Morize
Research Article

Abstract

The periodicity of striae formation in the tropical scallop Comptopallium radula (Indo-West Pacific Ocean) was investigated with an in situ marking technique, using the calcein fluorochrome. To minimize scallop stress caused by excessive handling, in situ benthic chambers were used for marking experiments. Once marked, scallops (shell height range: 38.4–75.8 mm) remained on site in a large benthic enclosure and were collected at regular time intervals to count new striae formed after marking, over a period of 3 months. A 3-h exposure period with calcein (150 mg l−1) was sufficiently long to create a detectable mark in nearly all shells. It was, however, impossible to count the striae in 48.2% of the shells (mainly large specimens) because of a very small growth after marking. Lack of significant mortality during the experiments indicated that tested calcein concentrations were not lethal. A decrease in shell growth rate was observed after marking but the respective impacts of calcein toxicity and changes in environmental conditions could not be discriminated. Our results suggest that in situ calcein marking inside benthic chambers is suitable for shell growth studies of scallops, provided the latter are not too old. After marking, the juvenile C. radula formed an average of one stria every 2.1 days in summer. Reports of 2-day periodicity in biological rhythms are rare. Striae formation in C. radula may be controlled by an endogenous oscillator, synchronized by an environmental cue acting as a zeitgeber, such as seawater temperature or sea level pressure, both of which exhibit 2-day variations in the Pacific Ocean. As in many other scallop species, C. radula forms striae periodically under natural conditions, but this study shows that in pectinid juveniles, this periodicity can deviate from a daily cycle. These results suggest that C. radula shells have tremendous potential for recording environmental conditions during periods ranging from months to a few years and with a resolution of 2 days.

Keywords

Calcein Immersion Time Oxygen Isotopic Composition Shell Growth Shell Height 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are especially grateful to Sandrine Chifflet for help during the preparation of calcein solutions, to Christophe Peignon and Alain Lapetite who performed part of SCUBA diving experimentation and subsequent field survey, and to the technical staff of US Chronos for their valuable help in preparing slides. We are also particularly grateful to Jennifer Guarini for her constructive comments and English corrections of the manuscript. This manuscript benefited from critical reviews by David Goodwin and three anonymous referees. This work was supported by IRD, the Programme National Environnement Côtier (PNEC) and ACI-PECTEN. It was part of a 3-year research program funded by IRD and the Région Bretagne. Contribution No. 978 of the IUEM, European Institute for Marine Studies (Brest, France).

References

  1. Antoine L (1978) La croissance journalière chez Pecten maximus (L.) (Pectinidae, Bivalvia). Haliotis 9:117–126Google Scholar
  2. Aschoff J, Daan S, Honma KI (1982) Zeitgeber, entrainment, and masking: some unsettled questions. In: Aschoff J, Daan S, Gross GA (eds) Vertebrate circadian system (structure and physiology). Springer, Berlin Heidelberg New York, pp 13–24CrossRefGoogle Scholar
  3. Breau L (2003) Etude de la bioaccumulation des métaux dans quelques espèces marines tropicales: recherche de bioindicateurs de contamination et application à la surveillance de l’environnement côtier dans le lagon sud-ouest de la Nouvelle-Calédonie. Thèse de Doctorat, Université de La RochelleGoogle Scholar
  4. Brooks RC, Heidinger RC, Kohler CC (1994) Mass-marking otoliths of larval and juvenile walleyes by immersion in oxytetracycline, calcein, or calcein blue. North Am J Fish Manage 14:143–150CrossRefGoogle Scholar
  5. Broom MJ, Mason J (1978) Growth and spawning in the pectinid Chlamys opercularis in relation to temperature and phytoplankton concentration. Mar Biol 47:277–285CrossRefGoogle Scholar
  6. Bumguardner BW, King TL (1996) Toxicity of oxytetracycline and calcein to juvenile striped bass. Trans Am Fish Soc 125:143–145CrossRefGoogle Scholar
  7. Bustamante P, Grigioni S, Boucher-Rodoni R, Caurant F, Miramand P (2000) Bioaccumulation of 12 trace elements in the tissues of the nautilus Nautilus macromphalus from New Caledonia. Mar Pollut Bull 40:688–696CrossRefGoogle Scholar
  8. Chauvaud L (1998) La coquille Saint-Jacques en rade de Brest: un modèle biologique d’étude des réponses de la faune benthique aux fluctuations de l’environnement. Thèse de Doctorat, Université de Bretagne OccidentaleGoogle Scholar
  9. Chauvaud L, Thouzeau G, Paulet Y-M (1998) Effects of environmental factors on the daily growth rate of Pecten maximus juveniles in the Bay of Brest (France). J Exp Mar Biol Ecol 227:83–111CrossRefGoogle Scholar
  10. Clark GR (1968) Mollusc shell: daily growth lines. Science 161:800–802CrossRefGoogle Scholar
  11. Clark GR (1975) Periodic growth and biological rhythms in experimentally grown bivalves. In: Rosenberg GD, Runcorn SK (eds) Growth rhythms and the history of the Earth’s rotation. Wiley, London pp 103–117Google Scholar
  12. Craig RL, Vincent RA, Fraser GJ, Smith MJ (1980) The quasi 2-day wave in the Southern Hemisphere mesosphere. Nature 287:319–320CrossRefGoogle Scholar
  13. Day RW, Williams MC, Hawkes GP (1995) A comparison of fluorochromes for marking abalone shells. Mar Freshw Res 46:599–605CrossRefGoogle Scholar
  14. Dijkstra HH (1984) Les pectinidae de Nouvelle-Calédonie: 5—Comptopallium radula. Rossiniana 24:11–12Google Scholar
  15. Epstein S, Buchsbaum R, Lowenstam HA, Urey HC (1953) Revised carbonate-water isotopic temperature scale. Bull Geol Soc Am 64:1315–1326CrossRefGoogle Scholar
  16. Fujikura K, Okoshi K, Naganuma T (2003) Strontium as a marker for estimation of microscopic growth rates in a bivalve. Mar Ecol Prog Ser 257:295–301CrossRefGoogle Scholar
  17. Gruffydd LD (1981) Observations on the rate of production of external ridges on the shell of Pecten maximus in the laboratory. J Mar Biolog Assoc U K 61:401–411CrossRefGoogle Scholar
  18. Hagan ME, Forbes JM, Vial F (1993) Numerical investigation of the propagation of the quasi-two-day wave into the lower thermosphere. J Geophys Res 98:23193–23205CrossRefGoogle Scholar
  19. Helm NE, Malouf RE (1983) Rate of production of the external ridges in the bay scallop, Argopecten irradians. Am Zool 23:835Google Scholar
  20. Hurley GV, Tremblay MJ, Couturier C (1987) Age estimation of sea scallop larvae (Placopecten magellanicus) from daily growth lines on shells. J Northwest Atl Fish Sci 7:123–129CrossRefGoogle Scholar
  21. Joll LM (1988) Daily growth rings in juvenile saucer scallops, Amusium balloti (Bernardi). J Shellfish Res 7:73–76Google Scholar
  22. Kaehler S, MacQuaid CD (1999) Use of the fluorochrome calcein as an in situ growth marker in the brown mussel Perna perna. Mar Biol 133:455–460CrossRefGoogle Scholar
  23. Kennish MJ, Olsson RK (1975) Effects of thermal discharges on the microstructural growth of Mercenaria mercenaria. Environ Geol 1:41–64CrossRefGoogle Scholar
  24. Kenyon KE (1996) Bi-daily variation of meteorological properties at sea level across the Pacific along 35°N. Atmos Res 43:31–46CrossRefGoogle Scholar
  25. Labrosse P, Fichez R, Farman R, Adams T (2000) New Caledonia. In: Sheppard C (eds) Seas at the millennium: an environmental evaluation. Elsevier, Amsterdam, pp 723–736Google Scholar
  26. Laing I (2000) Effect of temperature and ration on growth and condition of king scallop (Pecten maximus) spat. Aquaculture 183:325–334CrossRefGoogle Scholar
  27. Lefort Y (1994) Growth and mortality of the tropical scallops: Annachlamys flabellata (Bernardi), Comptopallium radula (Linne) and Mimachlamys gloriosa (Reeve) in southwest lagoon of New Caledonia. J Shellfish Res 13:539–546Google Scholar
  28. Lefort Y, Clavier J (1994) Reproduction of Annachlamys flabellata, Comptopallium radula and Mimachlamys gloriosa (Mollusca: Pectinidae) in the south-west lagoon of New Caledonia. Aquat Living Resour 7:39–46CrossRefGoogle Scholar
  29. Lorrain A (2002) Utilisation de la coquille Saint-Jacques comme traceur environnemental: approches biologique et biogéochimique. Thèse de Doctorat, Université de Bretagne OccidentaleGoogle Scholar
  30. MacFarlane GA, Beamish RJ (1987) Selection of dosages of oxytetracycline for age validation studies. Can J Fish Aquat Sci 44:905–909CrossRefGoogle Scholar
  31. Mages M, Woelfl S, Óvári M, v. Tümpling W Jr, Encina F (2004) The use of a portable total reflection X-ray fluorescence spectrometer for trace element determination in freshwater microcrustaceans (Daphnia). Spectrochim Acta B 59:1265–1272CrossRefGoogle Scholar
  32. Monaghan JP (1993) Comparison of calcein and tetracycline as chemical markers in summer flounder. Trans Am Fish Soc 122:298–301CrossRefGoogle Scholar
  33. Moran AL (2000) Calcein as a marker in experimental studies newly-hatched gastropods. Mar Biol 137:893–898CrossRefGoogle Scholar
  34. Owen R, Richardson C, Kennedy H (2002) The influence of shell growth rate on striae deposition in the scallop Pecten maximus. J Mar Biolog Assoc U K 82:621–623CrossRefGoogle Scholar
  35. Palmer JD (1975) Biological clocks of the tidal zone. Sci Am 232:70–79CrossRefGoogle Scholar
  36. Pannella G, MacClintock C (1968) Biological and environmental rhythms reflected in molluscan shell growth. J Paleontol 42(Mem. 2):64–80Google Scholar
  37. Parsons GJ, Robinson SMC, Roff JC, Dadswell MJ (1993) Daily growth rates as indicated by valve ridges in postlarval giant scallop (Placopecten magellanicus) (Bivalvia: Pectinidae). Can J Fish Aquat Sci 50:456–464CrossRefGoogle Scholar
  38. Pirker JG, Schiel DR (1993) Tetracycline as a fluorescent shell-marker in the abalone Haliotis iris. Mar Biol 116:81–86CrossRefGoogle Scholar
  39. Price GD, Pearce NJG (1997) Biomonitoring of pollution by Cerastoderma edule from the British Isles: a laser ablation ICP-MS study. Mar Pollut Bull 34:1025–1031CrossRefGoogle Scholar
  40. Richardson CA (2001) Molluscs as archives of environmental change. Oceanogr Mar Biol Annu Rev 39:103–164Google Scholar
  41. Richardson CA, Chenery SRN, Cook JM (2001) Assessing the history of trace metal (Cu, Zn, Pb) contamination in the North Sea through laser ablation—ICP-MS of horse mussel Modiolus modiolus shells. Mar Ecol Prog Ser 211:157–167CrossRefGoogle Scholar
  42. Rosenberg GD, Jones CB (1975) Approaches to chemical periodicities in molluscs and stromatolites. In: Rosenberg GD, Runcorn SK (eds) Growth rhythms and the history of the Earth’s rotation. Wiley, London, pp 223–242Google Scholar
  43. Rowley RJ, MacKinnon DI (1995) Use of the fluorescent marker calcein in biomineralisation studies of brachiopods and other marine organisms. Bull Inst Oceanogr Monaco Spec Issue 14:111–120Google Scholar
  44. Salby ML (1981) The 2-day wave in the middle atmosphere: observations and theory. J Geophys Res 86:9654–9660CrossRefGoogle Scholar
  45. Scherrer B (1984) Biostatistiques. Gaëtan Morin, QuébecGoogle Scholar
  46. Thompson I (1975) Biological clocks and shell growth in bivalves. In: Rosenberg GD, Runcorn SK (eds) Growth rhythms and the history of the Earth’s rotation. Wiley, London, pp 149–161Google Scholar
  47. Wallace JC, Reisnes TG (1985) The significance of various environmental parameters for growth of the iceland scallop, Chlamys islandica (Pectinidae), in hanging culture. Aquaculture 44:229–242CrossRefGoogle Scholar
  48. Wilson JH (1987) Environmental parameters controlling growth of Ostrea edulis L. and Pecten maximus L. in suspended culture. Aquaculture 64:119–131CrossRefGoogle Scholar
  49. Wilson CA, Beckman DW, Dean JM (1987) Calcein as a fluorescent marker of otoliths of larval and juvenile fish. Trans Am Fish Soc 116:668–670CrossRefGoogle Scholar
  50. Wrenn SL (1972) Daily increment formation and synchronization in the shell of the bay scallop. Am Zool 12:32Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Julien Thébault
    • 1
    • 2
  • Laurent Chauvaud
    • 2
  • Jacques Clavier
    • 2
  • Renaud Fichez
    • 3
  • Eric Morize
    • 4
  1. 1.IRD, Unité de Recherche CaméliaNouméa CedexNew Caledonia
  2. 2.IUEM-UBO, UMR CNRS 6539Technopôle Brest-IroisePlouzanéFrance
  3. 3.Centre d’Océanologie de MarseilleStation Marine d’EndoumeMarseilleFrance
  4. 4.IRD, US ChronosPlouzané CedexFrance

Personalised recommendations