Marine Biology

, Volume 148, Issue 3, pp 459–465 | Cite as

Daily and seasonal variations of optimum quantum yield and phenolic compounds in Cystoseira tamariscifolia (Phaeophyta)

  • R. T. Abdala-Díaz
  • A. Cabello-PasiniEmail author
  • E. Pérez-Rodríguez
  • R. M. Conde Álvarez
  • F. L. Figueroa
Research Article


Effects of solar radiation on phenolic compound concentrations and photosynthetic activity, estimated as in vivo chlorophyll fluorescence, in the brown alga Cystoseira tamariscifolia (Hudson) Papenfuss were analyzed in southern Spain from June 1988 to July 2000. Annual and diurnal variations of optimum quantum yield were negatively correlated with incident irradiance. Optimum quantum yield decreased as irradiance increased at noon, and yield values recovered in the afternoon suggesting a dynamic photoinhibition. The annual and daily fluctuations of phenolic compound concentration in the tissue of C. tamariscifolia showed contrasting patterns. There was an annual cycle of phenolic compound concentration in the apical thallus, which was positively correlated with incident irradiance. The increase in phenolic compounds, however, was twofold greater in the first half of the year than the decrease during the second half of the year. In contrast to the annual cycle, there appeared to be a negative correlation between phenolic compound concentration and irradiance in the summer months while no specific relationship was observed in the fall–winter months. Loss of phenolic compounds from the tissue to the surrounding water was increased as irradiation dosage increased. This suggests that the decrease of phenolic compounds during the diurnal cycle might be regulated by the exudation of these compounds at high irradiances in the field. Collectively, our results suggest that, like dynamic photoinhibition, the rapid synthesis and turnover time of phenolic compounds in the tissue of C. tamariscifolia might serve as photoprotective mechanisms against high irradiances.


Phenolic Compound Photosynthetically Active Radiation High Irradiance Brown Seaweed Irradiance Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank the Ministerio de Educación y Ciencia, Spain (CICYT AGL-2001-1888-C03-02) and Junta de Andalucía (Research group RNM-295) for financial support. Roberto Abdala-Díaz thanks AECI (Spain) and Alejandro Cabello-Pasini thanks the Spanish Ministry of Education and Science (SAB-2002-0209) for grants received. The authors are grateful for the use of the facilities of the Delegación Provincial (Consejería de Medio Ambiente, Junta de Andalucía) in the Natural Park “Cabo de Gata–Níjar”.


  1. Aquino-Bolaños EN, Mercado-Silva E (2004) Effects of polyphenol oxidase and preoxidase activity, phenolics and lignin content on the browning of cut jicama. Postharv Biol Technol 33:275–283CrossRefGoogle Scholar
  2. Arnold TM, Targett NM (2000) Evidence for metabolic turnover of polyphenolics in brown algae. J Chem Ecol 26:1393–1410CrossRefGoogle Scholar
  3. Arnold TM, Tanner CE, Hatch WI (1995) Phenotypic variation in polyphenolic content of the tropical alga Lobophora variegata as a function of nitrogen availability. Mar Ecol Progr Ser 123:177–183CrossRefGoogle Scholar
  4. Cabello-Pasini A, Muñiz-Salazar R, Ward DH (2004) Biochemical characterization of eelgrass (Zostera marina) at its southern end of distribution in the North Pacific. Cien Mar 30:21–34CrossRefGoogle Scholar
  5. Connan S (2004) Étude de la diversité spécifique des macroalgues de la Pointe de Bretagne et analyse des composés phenoliques des Phéophycées dominantes. PhD Dissertation, University of West BrittanyGoogle Scholar
  6. Conover JT, Sieburth JM (1964) Effect of Sargassum distribution on the epibiota and its antibacterial activity. Bot Mar 6:147–157CrossRefGoogle Scholar
  7. Conover JT, Sierbuth JM (1966) Effects of tannins secreted from Phaeophyta on planktonic animal survival in tidepools. Proc Int Seaweed Symp 5:99–100Google Scholar
  8. Craigie JS, McLachlan J (1964) Excretion of coloured ultraviolet absorbing substances by marine algae. Can J Bot 42:23–33CrossRefGoogle Scholar
  9. Figueroa FL, Salles S, Aguilera J, Jiménez C, Mercado J, Viñegla B, Flores-Moya A, Altamirano M (1997) Effects of solar radiation on photoinhibition and pigmentation in the red alga Porphyra leucosticta. Mar Ecol Progr Ser 151:81–90CrossRefGoogle Scholar
  10. Folin O, Ciocalteu V (1927). On tyrosine and tryptophane determinations in proteins. J Biol Chem 12:239–243Google Scholar
  11. Häder DP, Figueroa FL (1997) Photoecophysiology of marine macroalgae. Photochem Photobiol 66:1–14CrossRefGoogle Scholar
  12. Häder DP, Lebert M, Figueroa FL, Jiménez C, Viñegla B (1998) Photoinhibition in mediterranean macroalgae by solar radiation measured on site by PAM fluorescence. Aquat Bot 61:225–236CrossRefGoogle Scholar
  13. Hanelt D (1996) Photoinhibition of photosynthesis in marine macroalgae. Sci Mar 60:243–248Google Scholar
  14. Henry BE, Van Alstyne L (2004) Effects of UV radiation on growth and phlorotannins in Fucus gardneri (Phaeophyceae) juveniles and embryos. J Phycol 40:527–533CrossRefGoogle Scholar
  15. Osmond CB (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In: Baker NR, Bowyer JR (eds) Photoinhibition of photosynthesis, from molecular mechanisms to the field. Bios Scientific Publ Oxford, Oxford, pp 1–24Google Scholar
  16. Pavia H, Brock E (2000) Extrinsic factors influencing phlorotannin production in the brown alga Ascophyllum nodosum. Mar Ecol Prog Ser 193:285–294CrossRefGoogle Scholar
  17. Pavia H, Toth GB (2000) Influence of nitrogen on the phlorotannin content of the brown seaweeds Ascophyllum nodosum and Fucus vesiculosus. Hydrobiologia 440:299–305CrossRefGoogle Scholar
  18. Pavia H, Cervin G, Lindgren A, Åberg P (1997) Effects of UVB radiation and simulated herbivory on phlorotannins in the brown alga Ascophylum nodosum. Mar Ecol Prog Ser 157:139–46CrossRefGoogle Scholar
  19. Pérez-Rodríguez E, Gómez I, Figueroa FL (1998) Effects of UV radiation on photosynthesis and excretion of UV-absorbing pigments of Dasycladus vermicularis (Chlorophyta, Dasycladales) from Southern Spain. Phycologia 37:379–387CrossRefGoogle Scholar
  20. Pérez-Rodríguez E, Aguilera J, Gómez I, Figueroa FL (2001) Accumulation and excretion of coumarins in response to environmental stress by the Mediterranean green alga Dasycladus vermicularis. Mar Biol 139:633–639CrossRefGoogle Scholar
  21. Ragan MA, Glombitza KW (1986) Phlorotannins, brown alga polyphenols. In Round FE, Chapman DJ (eds) Prog Phycol Res, vol 4. Biopress, Bristol, England, pp 129–241Google Scholar
  22. Ragan MA, Jensen A (1978) Quantitative studies on brown algal phenols. II. Seasonal variation in polyphenol content of Ascophyllum nodosum (L.) Le Jol. and Fucus vesiculosus (L.). J Exp Mar Biol Ecol 34:245–258CrossRefGoogle Scholar
  23. Rodríguez-Martinez J (1979) Zooplancton de la Bahía de Málaga. Aproximación al conocimiento de una comunidad planctónica nerítica en el Mar de Alborán. PhD Dissertation, University of MalagaGoogle Scholar
  24. Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10:51–62CrossRefGoogle Scholar
  25. Sieburth JM, Jensen A (1969) Studies on algal substances in the sea. The formation of gelbstoff (humic material) by exudates of Phaeophyta. J Exp Mar Biol Ecol 3:275–289CrossRefGoogle Scholar
  26. Sokal RR, Rohlf FJ (1995) Biometry. W.H. Freeman and Company, New York, p 832Google Scholar
  27. Steinberg PD (1995) Seasonal-variation in the relationship between growth and phlorotannins production in the kelp Ecklonia radiata. Oecologia 102:169–173CrossRefGoogle Scholar
  28. Swanson AK, Druehl LD (2002) Induction, exudation and the UV protective role of kelp phlorotannins. Aquat Bot 73:241–253CrossRefGoogle Scholar
  29. Targett NM, Arnold TM (1998) Predicting the effects of brown algal phlorotannins on marine herbivores in tropical and temperate oceans. J Phycol 34:195–205CrossRefGoogle Scholar
  30. Van Alstyne KL (1988) Herbivore grazing increases polyphenolic defenses in the intertidal brown alga Fucus distichus. Ecology 69:655–663CrossRefGoogle Scholar
  31. Van Alstyne KL, Paul VJ (1990) The biogeography of polyphenolic compounds in marine macroalgae: temperate brown algal defenses deter feeding by tropical herbivorous fishes. Oceanologia 84:158–163Google Scholar
  32. Wallace G, Fry SC (1994) Phenolic components of the plant cell wall. Int Rev Cytol 151:229–267CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • R. T. Abdala-Díaz
    • 1
  • A. Cabello-Pasini
    • 2
    Email author
  • E. Pérez-Rodríguez
    • 1
  • R. M. Conde Álvarez
    • 1
  • F. L. Figueroa
    • 1
  1. 1.Departamento de Ecología, Facultad de CienciasUniversidad de MálagaMálagaSpain
  2. 2.Instituto de Investigaciones OceanológicasUniversidad Autónoma de Baja CaliforniaBaja CaliforniaMéxico

Personalised recommendations