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Marine Biology

, Volume 148, Issue 1, pp 87–96 | Cite as

δ15N as a natural tracer of particulate nitrogen effluents released from marine aquaculture

  • Sonja Lojen
  • Ehud Spanier
  • Anat Tsemel
  • Timor Katz
  • Noa Eden
  • Dror L. Angel
Research Article

Abstract

The flow of particulate nitrogen from marine net pen fish farm effluents to the surrounding biofouling community was quantified by means of stable isotopes of nitrogen. Plastic mesh substrates were deployed at 8 m depth near a sea bream fish farm and at a nearby reference site in the northern Gulf of Aqaba (Red Sea) to assess whether natural fouling organisms could sequester substantial quantities of farm-derived particulate nitrogen waste. A mixing equation, incorporating differences in nitrogen stable isotope composition, δ15N, between particulate organic matter (“source”) and fouling organisms (“sink”) at the fish farm and reference site, was used to estimate the amount of farm-derived nitrogen that was incorporated by the fouling community. Among the conspicuous fouling organisms examined, sponges, tunicates and polychaetes showed greatest uptake of fish farm N, where the mean fractions of farm-derived N estimated over the 2-year period of observation were 19±7, 22±6 and 31±8% of total organisms’ N content, respectively, with maximal recorded seasonal values of 68, 85 and 57%, respectively. Mean N uptake by mixed fouling communities (conspicuous + cryptic organisms) was as much as fivefold higher than that calculated for the sum of conspicuous taxa, suggesting that the retention efficiency is greater in mixed than in mono-specific biofouling communities.

Keywords

Reference Site Polychaete Particulate Organic Matter Fish Farm Sediment Trap 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was financed by the EU programme “Quality of Life and Management of Living Resources”, Contract No. Q5RS-2000-30305. The authors would like to acknowledge the kind assistance of the following groups and individuals in the field and laboratory work associated with this project: the Ardag fish farm, I. Lupatsch (Israel Oceanographic & Limnological Research), H. Lubinevsky (Haifa University), B. Čermelj (Marine Biology Station, National Institute of Biology, Slovenia), S. Žigon (J. Stefan Institute) and the P. Gschwend laboratory (Massachusetts Institute of Technology). All experiments performed in this study comply with the laws of the State of Israel and EU regulations.

References

  1. Adams TS, Sterner RW (2000) The effect of dietary nitrogen content on trophic level 15N enrichment. Limnol Oceanogr 45:601–607CrossRefGoogle Scholar
  2. Angel DL, Krost P, Gordin H (1995) Benthic implications of net cage aquaculture in the oligotrophic Gulf of Aqaba. Eur Aquac Soc Spec Publ 25:129–173Google Scholar
  3. Angel DL, Eden N, Breitstein S, Yurman A, Katz T, Spanier E (2002) In situ biofiltration: a means to limit the dispersal of effluents from marine finfish cage aquaculture. Hydrobiologia 469:1–10CrossRefGoogle Scholar
  4. Black KD (2001) Sustainability of aquaculture. In: Black KD (ed) Environmental impacts of aquaculture. Sheffield Academic Press, Sheffield, pp 199–212Google Scholar
  5. Bode A, Carrera P, Lens S (2003) The pelagic foodweb in the upwelling ecosystem of Galicia (NW Spain) during spring: natural abundance of stable carbon and nitrogen isotopes. ICES J Mar Sci 60:11–22CrossRefGoogle Scholar
  6. Bongiorni L, Shafir S, Angel D, Rinkevich B (2003) Survival, growth and reproduction of two hermaphytic corals subjected to in situ farm nutrient enrichment. Mar Ecol Prog Ser 253:137–144CrossRefGoogle Scholar
  7. Brenner S, Rosentroub Z, Bishop Y (1989) Current measurements in the Gulf of Eilat 1988/89. Report H8/89, Israel Oceanographic and Limnological Research, HaifaGoogle Scholar
  8. Costanzo SDO, Donohue MN, Dennison WC, Loneragan NR, Thomas M (2001) A new approach for detecting and mapping sewage impacts. Mar Pollut Bul 42:149–156CrossRefGoogle Scholar
  9. Cromey CJ (2004) MERAMOD—predictive model documentation. MERAMED Project Q5RS-2000–31779. http://www.meramed.com
  10. Eden N, Katz T, Angel DL (2003) Dynamic response of a mud snail Nassarius sinusigerus to changes in sediment biogeochemistry. Mar Ecol Prog Ser 263:139–147CrossRefGoogle Scholar
  11. Einen O, Holmefjord I, Asgard T, Talbot C (1995) Auditing nutrient discharges from fish farms: theoretical and practical considerations. Aquac Res 26:701–713CrossRefGoogle Scholar
  12. Iwama GK (1991) Interactions between aquaculture and the environment. Crit Rev Environ Control 21:177–216CrossRefGoogle Scholar
  13. Karakassis I, Tsapakis M, Hatziyanni E, Papadopoulou KN, Plaiti W (2000) Impact of cage farming of fish on the seabed in three Mediterranean coastal areas. ICES J Mar Sci 57:1462–1471CrossRefGoogle Scholar
  14. Karakassis I, Tsapakis M, Smith CJ, Rumohr H (2002) Fish farming impacts in the Mediterranean studied through sediment profiling imagery. Mar Ecol Prog Ser 227:125–133CrossRefGoogle Scholar
  15. Katz T, Herut B, Genin A, Angel DL (2002) Grey mullets ameliorate organically-enriched sediments below a fish farm in the oligotrophic Gulf of Aqaba (Red Sea). Mar Ecol Prog Ser 234:205–214CrossRefGoogle Scholar
  16. Kowalke J (2000) Ecology and energetics of two Antarctic sponges. J Exp Mar Biol Ecol 247:85–97CrossRefGoogle Scholar
  17. Lajtha K, Michener RM (eds) (1994) Stable Isotopes in Ecology and Environmental Sciences. Blackwell, New YorkGoogle Scholar
  18. Lefebvre S, Bacher C, Meuret A, Hussenot J (2001) Modelling nitrogen cycling in a mariculture ecosystem as a tool to evaluate its outflow. Estuar Coast Shelf Sci 52:305–325CrossRefGoogle Scholar
  19. Lumb CM (1989) Self-pollution by Scottish salmon farms? Mar Pollut Bull 20:375–379CrossRefGoogle Scholar
  20. Lupatsch I, Kissil GW (1998) Predicting aquaculture waste from gilthead seabream (Sparus aurata) culture using a nutritional approach. Aquat Living Resour 11:265–268CrossRefGoogle Scholar
  21. Malej A, Dolenec T, Lojen S (1998) Variability of 13C/12C and 15N/14N in different mussel tissues (Mytilus galloprovincialis): implications for food web studies. Rapp Com Int Mer Mediterr 35:272–273Google Scholar
  22. Marguillier S, van der Velde G, Dehairs F, Hemminga MA, Rajagopal S (1997) Trophic relationships in an interlinked mangrove-seagrass ecosystem as traced by δ13C and δ15N. Mar Ecol Prog Ser 151:115–121CrossRefGoogle Scholar
  23. McGhie TK, Crawford CM, Mitchell IM, O’Brien D (2000) The degradation of fish-cage waste during fallowing. Aquaculture 187:351–366CrossRefGoogle Scholar
  24. Minagawa M, Wada E (1984) Stepwise enrichment of 15N along food chains: further evidence and the relation between δ15N and animal age. Geochim Cosmochim Acta 48:1135–1140CrossRefGoogle Scholar
  25. Mook HD (1981) Removal of suspended particles by fouling communities. Mar Ecol Prog Ser 5:279–281CrossRefGoogle Scholar
  26. Pearson TH, Black KD (2001) The environmental impacts of marine fish cage culture. In: Black KD (ed) Environmental Impacts of Aquaculture. Sheffield Academic Press, Sheffield, pp 1–31Google Scholar
  27. Peterson BJ (1999) Stable isotopes as tracers of organic matter input and transfer in benthic food webs: a review. Acta Oecol 20:479–487CrossRefGoogle Scholar
  28. Peterson BJ, Howarth RW (1987) Sulfur, carbon, and nitrogen isotopes used to trace organic matter flow in the salt-marsh estuaries of Sapelo Island, Georgia. Limnol Oceanogr 32:1195–1213CrossRefGoogle Scholar
  29. Phillips DL (2001) Mixing models in analyses of diet using multiple stable isotopes: a critique. Oecologia 127:166–170CrossRefGoogle Scholar
  30. Phillips DL, Gregg JW (2001) Uncertainty in source partitioning using stable isotopes. Oecologia 127:171–179CrossRefGoogle Scholar
  31. Pile AJ, Patterson AJ, Savarese M, Chernykh VI, Fialkov VA (1997) Trophic effects of sponge feeding within Lake Baikal’s littoral zone, 2. Sponge abundance, diet, feeding efficiency, and carbon flux. Limnol Oceanogr 42:178–184CrossRefGoogle Scholar
  32. Qian PY, Wu MCS, Ni IH (2001) Comparison of nutrients release among some maricultured animals. Aquaculture 200:305–316CrossRefGoogle Scholar
  33. Raikow DF, Hamilton SK (2001) Bivalve diets in a Midwestern U.S. stream: a stable isotope enrichment study. Limnol Oceanogr 46:514–522CrossRefGoogle Scholar
  34. Reiss Z, Hottinger L (1984) The Gulf of Aqaba (Eilat): ecological micropaleontology. Ecological Studies, vol 50. Springer, Berlin Heidelberg New YorkGoogle Scholar
  35. Reiswig HM (1971) Particle feeding in natural populations of three marine demosponges. Biol Bul (Woods Hole) 141:568–591CrossRefGoogle Scholar
  36. Reiswig HM (1974) Water transport, respiration and energetics of three tropical marine sponges. J Exp Mar Biol Ecol 14:231–249CrossRefGoogle Scholar
  37. Riera P, Stal LJ, Nieuwenhuize J (2002) δ13C versus δ15N of co-occurring molluscs within a community dominated by Crassostrea gigas and Crepidula fornicata (Oosterschelde, The Netherlands). Mar Ecol Prog Ser 240:291–295CrossRefGoogle Scholar
  38. Riisgård HU (1991) Suspension feeding in the polychaete Nereis Diversicolor. Mar Ecol Prog Ser 70:29–37CrossRefGoogle Scholar
  39. Ruiz JM, Pérez M, Romero J (2001) Effects of fish farm loadings on seagrass (Posidonia oceanica) distribution, growth and photosynthesis. Mar Pollut Bull 42:749–760CrossRefGoogle Scholar
  40. Sarà G, Scilipoti D, Mazzola A, Modica A (2004) Effects of fish farming waste to sedimentary and particulate organic matter in a southern Mediterranean area (Gulf of Castellammare, Sicily): a multiple stable isotope study (δ13C and δ15N). Aquaculture 234:199–213CrossRefGoogle Scholar
  41. Schwarcz HP (1991) Some theoretical aspects of isotope paleodiet studies. J Archaeol Sci 18:261–276CrossRefGoogle Scholar
  42. Spanier E, Tsemel A, Lubinevski H, Roitemberg A, Yurman A, Breitstein S, Angel D, Eden N, Katz T (2003) Can open water bio-filters be used for the reduction of the environmental impact of finfish net cage aquaculture in the coastal waters of Israel? Annales Ser Hist Nat 13:25–28Google Scholar
  43. Vedel A, Andersen BB, Riisgård HU (1994) Field investigations of pumping activity of the facultatively filter-feeding polychaete Nereis diversicolor using an improved infrared phototransducer system. Mar Ecol Prog Ser 103:91–101CrossRefGoogle Scholar
  44. Wada E, Mizutani H, Minagawa M (1991) The use of stable isotopes for food web analysis. Crit Rev Food Sci Nutr 30:361–371CrossRefGoogle Scholar
  45. Wada E, Kabaya Y, Kurihara Y (1993) Stable isotopic structure of aquatic organisms. J Biosci 18:483–499CrossRefGoogle Scholar
  46. Ye LX, Ritz DA, Fenton GE, Lewis ME (1991) Tracing the influence on sediments of organic waste from a salmonid farm using stable isotope analysis. J Exp Mar Biol Ecol 145:161–174CrossRefGoogle Scholar
  47. Yoshii K, Melnik N, Timoshkin OA, Bondarenko NA, Anoshko PN, Yoshioka T, Wada E (1999) Stable isotope analyses of the pelagic food web in Lake Baikal. Limnol Oceanogr 44:502–511CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Sonja Lojen
    • 1
  • Ehud Spanier
    • 2
  • Anat Tsemel
    • 2
  • Timor Katz
    • 3
  • Noa Eden
    • 3
  • Dror L. Angel
    • 2
  1. 1.Department of Environmental SciencesJožef Stefan InstituteLjubljanaSlovenia
  2. 2.The Leon Recanati Institute for Maritime Studies and Department of Maritime CivilizationsUniversity of HaifaMount CarmelIsrael
  3. 3.The Interuniversity Institute for Marine Sciences in EilatEilatIsrael

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