Marine Biology

, Volume 147, Issue 5, pp 1141–1148

A multi-locus genetic assignment technique to assess sources of Agaricia agaricites larvae on coral reefs

  • Daniel A. Brazeau
  • Paul W. Sammarco
  • Daniel F. Gleason
Research Article

DOI: 10.1007/s00227-005-0022-5

Cite this article as:
Brazeau, D.A., Sammarco, P.W. & Gleason, D.F. Marine Biology (2005) 147: 1141. doi:10.1007/s00227-005-0022-5


Assignment of individuals to populations based upon genetic data is an important ecological problem that requires many polymorphic markers, often more than are available using single locus techniques. To demonstrate the utility of amplified fragment length polymorphisms (AFLP) in studying larval dispersal and recruitment in coral populations, two sets of AFLP primers were used to genotype colonies of the coral Agaricia agaricites Linnaeus from three widely separated geographic locations: the Bahamas (23°28′N, 75°42′W) and Key Largo, Florida (24°55′N, 80°31′W—two sites separated by 12 km) in 1995, and the Flower Garden Banks (FGB) in the Gulf of Mexico (27°55′N,93°36′W) in 1997. In addition to adult samples from each site, recruits were collected from settling plates placed on the East FGB for 1 year (1997–1998). The AFLP technique yielded 45 polymorphic markers. An analysis of molecular variance (AMOVA) showed significant genetic differences among the four adult populations, even between the two Key Largo sites. The recruits were significantly different from all adult populations except those from the FGB. Discriminant function analysis and the program AFLPOP were used to assign individuals to populations. Using the adult AFLP-banding patterns to build the statistical models, both procedures correctly assigned the majority of adults to their respective populations in simulations and assigned all but one of the recruits to the Flower Garden population from where they were collected . The AFLP technique provides a simple and adaptable population assignment method for studying recruitment processes in A. agaricites and other coral species.

Supplementary material

227_2005_22_MOESM1_ESM.pdf (166 kb)
Supplemental Appendix

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Daniel A. Brazeau
    • 1
  • Paul W. Sammarco
    • 2
  • Daniel F. Gleason
    • 3
  1. 1.Department of Pharmaceutical SciencesUniversity at BuffaloBuffaloUSA
  2. 2.Louisiana Universities Marine Consortium (LUMCON)ChauvinUSA
  3. 3.Department of BiologyGeorgia Southern UniversityStatesboroUSA

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