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Marine Biology

, Volume 146, Issue 5, pp 869–875 | Cite as

Morphological evidence for vertical transmission of symbiotic bacteria in the viviparous sponge Halisarca dujardini Johnston (Porifera, Demospongiae, Halisarcida)

  • Alexander V. EreskovskyEmail author
  • Elizaveta Gonobobleva
  • Andrey Vishnyakov
Research Article

Abstract

All stages of vertical transmission of symbiotic bacteria, from the penetration into oocytes to the formation of rhagon, were investigated in the White Sea (Arctic) representatives of Halisarca dujardini Johnston (Demospongiae). Small populations of free-living specific symbiotic bacteria inhabit the mesohyl of H. dujardini. They are represented by a single morphotype of small spiral gram-positive bacteria. Vertical transmission of symbiotic bacteria between generations in sponges may occur in different ways. In the case of H. dujardini the bacteria penetrate into growing oocytes by endocytosis. A part of the bacteria plays a trophic role for oocytes and the other part remains undigested in membrane-bound vacuoles within the cytoplasm. In cleaving embryos bacteria are situated between the blastomeres or in the vacuoles. In the blastula all bacteria are disposed in the blastocoel. The symbionts are situated in intercellular spaces in free-swimming larvae and during metamorphosis. Symbiotic bacteria do not play any trophic role in the period of embryonic and postembryonic development of H. dujardini. No signs of destruction and digestion of bacteria were revealed at any stage of development.

Keywords

Sponge Vertical Transmission Marine Sponge Symbiotic Bacterium Yolk Granule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors thank Dr. Jean Vacelet and Nicole Boury-Esnault for helpful discussions, and Chantal Bézac for help during the SEM preparation. This work was funded by grant INTAS-YSC 02-4441, by the program Universities of Russia no. 07.01.017, and by grant RFBR no. 03-04-49773; A. Ereskovsky received a special grant from the Federal Scientific Policy of Belgium, intended to promote collaboration S&T with oriental and central Europe.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Alexander V. Ereskovsky
    • 1
    Email author
  • Elizaveta Gonobobleva
    • 1
  • Andrey Vishnyakov
    • 1
  1. 1.Department of Embryology, Biological FacultySt. Petersburg State UniversitySt. PetersburgRussia

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