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Marine Biology

, Volume 145, Issue 3, pp 551–561 | Cite as

Anatomical and experimental evidence for particulate feeding in Lucinoma aequizonata and Parvilucina tenuisculpta (Bivalvia: Lucinidae) from the Santa Barbara Basin

  • M. R. DuplessisEmail author
  • S. C. Dufour
  • L. E. Blankenship
  • H. Felbeck
  • A. A. Yayanos
Research Article

Abstract

Previous nutritional models for adults of the lucinid bivalve Lucinoma aequizonata contend that symbiotic chemoautotrophic bacteria provide most of the organic carbon for the host. The existence of this symbiosis, coupled with the host’s distinctive anatomical features, shaped the impression that particulate feeding was not a significant part of L. aequizonata nutrition. Here, we use several techniques to show that particulate feeding is a consistent and important part of the L. aequizonata nutritional strategy. Histological and scanning electron microscopy observations reveal that the gills of L. aequizonata, like those of the lucinid Parvilucina tenuisculpta, have functional mucociliary epithelia, able to transport captured particles to the mouth. Observations of gut content and radiolabeled feeding experiments indicate that L. aequizonata does ingest and assimilate carbon from particulate organic matter. Furthermore, molecular identification of a broad spectrum of organisms in the guts of native adult specimens demonstrates that L. aequizonata is non-selective when ingesting organic material, and has a mixotrophic diet.

Keywords

Bivalve Diatom Frustule Visceral Mass Labial Palp Ventral Groove 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank J. Robidart, V. Bowden, and F. Malfatti for help aboard the R.V. “Sproul”, as well as C. Johnson for providing the Dunaliella tertiolecta culture. P.V. Scott helped with bivalve identifications. M. Craig helped with the phylogenetic analysis of gut sequences. R. Chastain, D. Woodruff, B. Brahamsha, and R. Burton all provided suggestions regarding technical aspects of the molecular diet analysis. Special thanks to the captain and crew of the R.V. “Robert Gordon Sproul”. Funding for this research was provided by NSF grants OCE 99-07651 to A.A.Y., OCE 99-07810 to H.F. and J. Stein, a University of California President Dissertation Fellowship and E.W. Scripps Fellowship to M.R.D., an NSERC postgraduate scholarship and a Baxter and Alma Ricard Foundation Fellowship to S.C.D. The experiments comply with the current laws in the USA.

Supplementary material

Table S1 Closest identified relatives of prokaryote 16S rRNA and eukaryote 18S rRNA sequences amplified from Lucinoma aequizonata guts

supp.pdf (250 kb)
(PDF 250 KB)

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • M. R. Duplessis
    • 1
    • 2
    Email author
  • S. C. Dufour
    • 1
  • L. E. Blankenship
    • 1
  • H. Felbeck
    • 1
  • A. A. Yayanos
    • 1
  1. 1.Marine Biology Research DivisionScripps Institution of OceanographyLa JollaUSA
  2. 2.Department of BiologySeattleUSA

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