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Marine Biology

, Volume 144, Issue 3, pp 593–603 | Cite as

Non-random mating and population genetic subdivision of two broadcasting corals at Ningaloo Reef, Western Australia

Research Article

Abstract

Allozyme electrophoresis of two corals was used to assess whether populations at Ningaloo Reef, Western Australia are primarily self-seeding or whether recruitment is from a broader geographic pool. Significant genetic subdivision across a range of spatial scales (between 6.5 km and 155 km) was found for both Acropora digitifera and A. aspera, with mean F ST values of 0.010 and 0.067 respectively. Large departures from Hardy-Weinberg expectations were found for both species. Without exception these were due to deficits of heterozygotes; mean D values were −0.341 for A. digitifera and −0.455 for A. aspera. The magnitude of the deficits was consistent both across loci for all sites and across all sites for each locus. Some loci were found to be in linkage disequilibrium but no consistent pattern was observed. Also, multi-locus genotypic diversity values were generally high (between 0.83 and 1.00) and so departures from equilibria cannot be attributed to asexual reproduction. The most plausible explanation for the patterns observed is restricted gene flow at both the planktonic and gametic stages, with mating between close relatives.

Keywords

Great Barrier Reef Coral Cover Asexual Reproduction Neap Tide Restricted Gene Flow 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This research was funded by the Department of Zoology, The University of Western Australia, Mobil Exploration and Producing, Woodside Petroleum, Exmouth Shire, the Conservation, Animal rescue, Research and Education group of Exmouth, and Kailis Fisheries. Thanks to many dive buddies, Conservation and Land Management and B. Lefroy for accommodation, L. Marsh for coral identification, M. Forde for the use of his map of Ningaloo, J. Stoddart for his BINOM program and R. Black for kindly modifying the program to run on a Macintosh. This paper benefited greatly from comments by my supervisor M. Johnson, and two anonymous referees. The collection of corals and the genetic analyses comply with the laws of Australia.

References

  1. Ayre DJ, Dufty S (1994) Evidence for restricted gene flow in the viviparous coral Seriatopora hystrix on Australia’s Great Barrier Reef. Evolution 48:1183–1201Google Scholar
  2. Ayre DJ, Hughes TP (2000) Genotypic diversity and gene flow in brooding and spawning corals along the Great Barrier Reef, Australia. Evolution 54:1590–1605PubMedGoogle Scholar
  3. Ayre DJ, Hughes TP, Standish RJ (1997a) Genetic differentiation, reproductive mode, and gene flow in the brooding coral Pocillopora damicornis along the Great Barrier Reef, Australia. Mar Ecol Prog Ser 159:175–187Google Scholar
  4. Ayre DJ, Davis AR, Billingham M, Llorens T, Styan C (1997b) Genetic evidence for contrasting patterns of dispersal in solitary and colonial ascidians. Mar Biol 130:51–61CrossRefGoogle Scholar
  5. Babcock RC, Heyward AJ (1986) Larval development of certain gamete-spawning scleractinian corals. Coral Reefs 5:111–116Google Scholar
  6. Babcock RC, Bull GD, Harrison PL, Heyward AJ, Oliver JK, Wallace CC, Willis BL (1986) Synchronous spawnings of 105 scleractinian coral species on the Great Barrier Reef. Mar Biol 90:379–394Google Scholar
  7. Bak RPM, Engel MS (1979) Distribution, abundance and survival of juvenile hermatypic corals (Scleractinia) and the importance of life history strategies in the parent coral community. Mar Biol 54:341–352Google Scholar
  8. Billingham M, Ayre DJ (1996) Genetic subdivision in the subtidal, clonal sea anemone Anthothoe albocincta. Mar Biol 125:153–163Google Scholar
  9. Black KP (1993) The relative importance of local retention and inter-reef dispersal of neutrally buoyant material on coral reefs. Coral Reefs 12:43–53Google Scholar
  10. Black KP, Moran PJ (1991) Influence of hydrodynamics on the passive dispersal and initial recruitment of larvae of Acanthaster planci on the Great Barrier Reef. Mar Ecol Prog Ser 69:55–65Google Scholar
  11. Brown LD (1991) Genetic variation and population structure in the blacklip abalone, Haliotis rubra. Aust Mar Freshw Res 42:77–90Google Scholar
  12. Bull G (1986) Distribution and abundance of coral plankton. Coral Reefs 4:197–200Google Scholar
  13. Burnett WJ, Benzie JAH, Beardmore JA, Ryland JS (1994) High genetic variability and patchiness in a common Great Barrier Reef zoanthid (Palythoa caesia). Mar Biol 121:153–160Google Scholar
  14. Burnett WJ, Benzie JAH, Beardmore, JA, Ryland JS (1995) Patterns of genetic subdivision in populations of a clonal cnidarian, Zoanthus coppingeri, from the Great Barrier Reef. Mar Biol 122:665–673Google Scholar
  15. Burton RS (1986) Evolutionary consequences of restricted gene flow in the intertidal copepod Tigriopus californicus. Bull Mar Sci 39:526–535Google Scholar
  16. Crisp JD (1978) Genetic consequences of different reproductive strategies in marine invertebrates In: Battaglia B, Beardmore JA (eds) Marine organisms: genetics, ecology and evolution. Plenum, New York, pp 257–273Google Scholar
  17. Dai CF, Fan TY, Yu JK (2000) Reproductive isolation and genetic differentiation of a scleractinian coral Mycedium elephantotus. Mar Ecol Prog Ser 201:179–187Google Scholar
  18. Done TJ (1982) Patterns in the distribution of coral communities across the central Great Barrier Reef. Coral Reefs 1:95–107Google Scholar
  19. Forde MJ (1994) Ecology of the muricid gastropod Drupella cornus (Röding 1798) and its significance as a corallivore on Ningaloo Reef, Western Australia. MSc thesis, University of Western AustraliaGoogle Scholar
  20. Harrison PL, Wallace CC (1990) Reproduction, dispersal and recruitment of scleractinian corals. In: Dubinsky Z (ed) Ecosystems of the world 25. Coral Reefs. Elsevier, Amsterdam, pp 133–207Google Scholar
  21. Harrison PL, Babcock RC, Bull GD, Oliver JK, Wallace CC, Willis BL (1984) Mass spawning in tropical reef corals. Science 223:1186–1189Google Scholar
  22. Hearn CJ, Parker IN (1988) Hydrodynamic processes on the Ningaloo coral reef, Western Australia. Proceedings of 6th International Coral Reef Symposium, Townsville 2:497–502Google Scholar
  23. Hellberg ME (1996) Dependence of gene flow on geographic distance in two solitary corals with different larval dispersal capabilities. Evolution 50:1167–1175Google Scholar
  24. Hilbish TJ (1996) Population genetics of marine species: the interaction of natural selection and historically differentiated populations. J Exp Biol Mar Ecol 200:67–83CrossRefGoogle Scholar
  25. Holborn K, Johnson MS, Black R (1994) Population genetics of the corallivorous gastropod Drupella cornus at Ningaloo Reef, Western Australia. Coral Reefs 13:33–39Google Scholar
  26. Hunt A (1993) Effects of contrasting patterns of larval dispersal on the genetic connectedness of local populations of two intertidal starfish, Patiriella calcar and P. exigua. Mar Ecol Prog Ser 92:179–186Google Scholar
  27. Johnson MS, Black R (1982) Chaotic genetic patchiness in an intertidal limpet, Siphonaria sp. Mar Biol 70:157–164Google Scholar
  28. Johnson MS, Black R (1984a) Pattern beneath the chaos: the effect of recruitment on genetic patchiness in an intertidal limpet. Evolution 38: 1371–1383Google Scholar
  29. Johnson MS, Black R (1984b) The Wahlund effect and the geographical scale of variation in the intertidal limpet Siphonaria sp. Mar Biol 79:295–302Google Scholar
  30. Johnson MS, Threlfall TJ (1987) Fissiparity and population genetics of Coscinasterias calamaria. Mar Biol 93:517–525Google Scholar
  31. Johnson MS, Holborn K, Black R (1993) Fine-scale patchiness and genetic heterogeneity of recruits of the corallivorous gastropod Drupella cornus. Mar Biol 117:91–96Google Scholar
  32. Jokiel PL (1984) Long distance dispersal of reef corals by rafting. Coral Reefs 3:113–116Google Scholar
  33. Kawaguti S (1940) An abundance of reef-coral planulae in the plankton. Zool Mag (Tokyo) 52:31Google Scholar
  34. Li CC (1976) First course in population genetics. Boxwood, Pacific Grove, Calif.Google Scholar
  35. McFadden CS, Grosberg RK, Cameron BB, Karlton DP, Secord D (1997) Genetic relationships within and between clonal and solitary forms of the sea anemone Anthopleura elegantissima revisted: evidence for the existence of two species. Mar Biol 128:127–139CrossRefGoogle Scholar
  36. Palumbi SR (1994) Genetic divergence, reproductive isolation, and marine speciation. Annu Rev Ecol Syst 25:547–572CrossRefGoogle Scholar
  37. Pearson EO, Hartley HO (1956) Biometrika tables for statisticians, vol 1. Cambridge University Press, CambridgeGoogle Scholar
  38. Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  39. Reynolds J, Weir BS, Cockerham CC (1983) Estimation of the coancestry coefficient: basis for a short-term genetic distance. Genetics 105:767–779Google Scholar
  40. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225Google Scholar
  41. Richardson BJ, Baverstock PR, Adams M (1986) Allozyme electrophoresis. A handbook for animal systematics and population studies. Academic Press, LondonGoogle Scholar
  42. Sammarco PW (1991) Geographically specific recriutment and post-settlement mortality as influences on coral communities: the cross-continental shelf transplant experiment. Limnol Oceanogr 36:496–514Google Scholar
  43. Scheltema RS (1975) Relationship of larval dispersal, gene-flow, and natural selection to geographic variation of benthic invertebrates in estuaries and along coastal regions. In: Cronin LE (ed) Estuarine research, vol 1. Academic Press, New York, pp 372–391Google Scholar
  44. Selander RK (1970) Behavior and genetic variation in natural populations. Am Zool 10:53–66Google Scholar
  45. Selander RK, Smith MH, Yang SH, Johnson WE, Gentry JB (1971) Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-field mouse (Peromyscus polionotus). Stud Genet 6:49–90Google Scholar
  46. Simpson CJ (1985) Mass spawning of scleractinian corals in the Dampier Archipelago and the implications for management of coral reefs in Western Australia. Bulletin 244. Department of Conservation and Environment Perth, Western AustraliaGoogle Scholar
  47. Stoddart JA (1983) Asexual production of planulae in the coral Pocillopora damicornis. Mar Biol 76:279–284Google Scholar
  48. Stoddart JA (1984) Genetical structure within populations of the coral Pocillopora damicornis. Mar Biol 81:19–30Google Scholar
  49. Stoddart JA (1988) Historecognition and fine-scale spatial genetic structure in sessile benthic invertebrates. In: Grosberg RK, Hedgecock D, Nelson K (eds) Invertebrate historecognition. Plenum, New York, pp 111–125Google Scholar
  50. Stoddart JA (1989) Fatal attraction. Landscope 4(4): 14–20Google Scholar
  51. Swofford DL, Selander RB (1981) BIOSYS-1. A FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J Hered 72:281–283Google Scholar
  52. Veron JEN (1993) Corals of Australia and the Indo-Pacific, 2nd edn. University of Hawaii Press, HonoluluGoogle Scholar
  53. Wallace CC (1985) Reproduction, recruitment and fragmentation in nine sympatric species of the coral genus Acropora. Mar Biol 88:217–233Google Scholar
  54. Wallace CC, Willis BL (1994) Systematics of the coral genus Acropora: Implications of new biological findings for species concepts. Annu Rev Ecol Syst 25:237–262Google Scholar
  55. Weir BS (1990) Genetic data analysis. Sinauer, Sunderland, Mass.Google Scholar
  56. Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370Google Scholar
  57. Whitaker K (1997) Dispersal and recolonization potential of five species of hermatypic corals (Scleractinia) at Ningaloo Reef, Western Australia. PhD thesis, University of Western AustraliaGoogle Scholar
  58. Whitlock MC, McCauley DE (1999) Indirect measures of gene flow and migration: FST≠1/(4Nm+1). Heredity 82:117–125PubMedGoogle Scholar
  59. Williams DMcB, Wolanski E, Andrews JC (1984) Transport mechanisms and the potential movement of planktonic larvae in the central region of the Great Barrier Reef. Coral Reefs 3:229–236Google Scholar
  60. Willis BL, Oliver JK (1988) Inter-reef dispersal of coral larvae following the annual mass spawning on the Great Barrier Reef. Proceedings of the 6th International Coral Reef Symposium, Australia 2:853–859Google Scholar
  61. Workman PL, Niswander JD (1970) Population studies on southwestern Indian tribes. II. Local genetic differentiation in the Papago. Am J Hum Genet 22:24–29PubMedGoogle Scholar
  62. Wright S (1978) Evolution and genetics of populations, vol 4. Variability within and among natural populations. University of Chicago Press, ChicagoGoogle Scholar
  63. Yates F (1934) Contingency tables involving small numbers and the χ 2 test. J R Stat Soc [Suppl] 1:217–235Google Scholar
  64. Zouros E, Foltz DW (1984) Possible explanations of heterozygote deficiency in bivalve molluscs. Malacologia 25:583–591Google Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Department of ZoologyUniversity of Western AustraliaNedlandsAustralia
  2. 2.School of Natural Resource SciencesQueensland University of TechnologyBrisbaneAustralia

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