Marine Biology

, Volume 140, Issue 6, pp 1235–1240

Participation of the C-type hemolymph lectin in mineralization of the acorn barnacle Megabalanusrosa

  •  H. Kamiya
  •  M. Jimbo
  •  H. Yako
  •  K. Muramoto
  •  O. Nakamura
  •  R. Kado
  •  T. Watanabe

DOI: 10.1007/s00227-002-0789-6

Cite this article as:
Kamiya, H., Jimbo, M., Yako, H. et al. Marine Biology (2002) 140: 1235. doi:10.1007/s00227-002-0789-6

Abstract.

BRA-2, a major C-type lectin in the hemolymph of the acorn barnacle Megabalanusrosa was detected in shell-associated proteins. Immobilized BRA-2 promoted CaCO3 crystallization as determined by an in vitro pH-drift mineralization assay. In nature, calcification occurs as wound repair in M. rosa after breakage of the shell plate. To observe this phenomenon, we inserted a rubber cube inside the mantle cavity of M. rosa through the basal plate, and kept the organisms in a tank for several weeks. The rubber cube was covered first with brownish-colored material, probably a melanin, and then with CaCO3 5 weeks after the insertion. In the CaCO3-associated proteins, BRA-3, a minor component of M. rosa hemolymph lectins, was present in addition to BRA-2. The presence of both the lectins and their ligands on the hemocyte was also observed. These results suggest that the C-type lectins of the M. rosa hemolymph participate in mineralization as well as defense in these organisms.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  H. Kamiya
    • 1
  •  M. Jimbo
    • 1
  •  H. Yako
    • 1
  •  K. Muramoto
    • 2
  •  O. Nakamura
    • 1
  •  R. Kado
    • 1
  •  T. Watanabe
    • 1
  1. 1.School of Fisheries Sciences, Kitasato University, Ofunato, Iwate 022-0101, Japan
  2. 2.Department of Biological Resource Sciences, Graduate School of Agriculture, Tohoku University, Sendai, Miyagi 981-8555, Japan

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