Response surface methodology for optimisation of total polyphenol content and antioxidant activity of extracts from Maytenus macrocarpa bark by means of ultrasound-assisted extraction
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The aim of this study was to optimise the ultrasound-assisted extraction of the total polyphenol content (TPC) and antioxidant activity from Maytenus macrocarpa bark by means of response surface methodology (RSM). The effect and interactions of temperature, time, particle size, solid:solvent ratio and water:ethanol ratio were analysed by using a fractional factorial design type 25−1. The most significant factors were: temperature, particle size and time. The RSM was applied to the optimisation of the TPC and two total antioxidant activities [Ferric reducing antioxidant power (FRAP) and 2,2 -azino-bis (3-ethylbenthiozoline-6-sulphonic acid) (ABTS)] as response variables. Four polynomial models were applied; the quadratic model was the most adequate one, with an adjusted R2 value of 0.9422. M. macrocarpa has a considerable TPC that contributes to its antioxidant activity. The best results from the analysis of correlations were found in the FRAP versus TPC and ABTS versus FRAP, with a Pearson’s r coefficient of 0.961 and 0.953, respectively.
This work was supported by Universidad Estatal Amazónica (UEA-AGR-003-2015).
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Conflicts of interest
The authors declare no conflicts of interest.
- Anderson MJ, Whitcomb PJ (2016) DOE simplified: practical tools for effective experimentation. CRC Press, Boca RatonGoogle Scholar
- ASTM-E1757-01 (2007) Standard practice for preparation of biomass for compositional analysis. ASTM International, West ConshohockenGoogle Scholar
- ASTM-E871-82 (2006) Standard test method for moisture analysis of particulate wood fuels. ASTM International, West ConshohockenGoogle Scholar
- Du G, Zhao HY, Zhang QW, Li GH, Yang FQ, Wang Y, Li YC, Wang YT (2010) A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry. J Chromatogr A 1217:705–714. https://doi.org/10.1016/j.chroma.2009.12.017 CrossRefPubMedGoogle Scholar
- Garden MB (2017) Maytenus Missouri Botanical Garden. http://www.tropicos.org/NameSearch.aspx?name=Maytenus&commonname. Accessed 15 June 2017
- Piacente S, Dos Santos LC, Mahmood N, Pizza C (2006) Triterpenes from Maytenus macrocarpa and evaluation of their anti-HIV activity Nat Prod Commun 19:1073–1078Google Scholar
- Rommel Z-H, Alexander Z-H, Karelya W-P et al. (2016) Study on the Temperature, respiratory rate, heart rate and Electrocardiogram of concomitant administration of Maytenus macrocarpa “Chuchuhuasi” and Propranolol in escalating doses. Pharm Commun 6Google Scholar
- Singleton V, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158Google Scholar
- Veloso CC, Soares GL, Perez AC, Rodrigues VG, Silva FC (2017) Pharmacological potential of Maytenus species and isolated constituents, especially tingenone, for treatment of painful inflammatory diseases. Revista Brasileira de Farmacognosia 27:533–540. https://doi.org/10.1016/j.bjp.2017.02.006 CrossRefGoogle Scholar
- Whitcomb P, Oehlert GW (2007) Graphical selection of effects in general factorials. Fall Techn Conf 612:2036Google Scholar
- Witek-Krowiak A, Chojnacka K, Podstawczyk D, Dawiec A, Pokomeda K (2014) Application of response surface methodology and artificial neural network methods in modelling and optimization of biosorption process. Bioresour Technol 160:150–160. https://doi.org/10.1016/j.biortech.2014.01.021 CrossRefPubMedGoogle Scholar