Effect of alcohol-based organosolv treatment combined with short-time ball milling on the enzymatic hydrolysis of Japanese cypress (Chamaecyparis obtusa)
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Japanese cypress (Chamaecyparis obtusa) is a recalcitrance softwood widely used as building material in Japan. A combined technique of alcohol-based organosolv treatment and ball milling (BM) of this softwood for the production of fermentable sugars from lignocellulose was investigated. Ethanol, ethylene glycol, water, and their mixtures were used as solvents, and acetic acid was used as a proton donor. After solvolysis, a residual sample was subjected to short-time BM, and the entire residue was evaluated by saccharification with Acremonium cellulase. Short-time BM after alcohol-based organosolv treatment greatly improved the enzymatic digestibility and decreased the required severity of organosolv treatment. Moreover, alcohol-based organosolv treatment increased the efficiency and reduced the time required for BM despite small quantity of removed lignin. It was found that the combination of alcohol-based organosolv treatment in mild condition and short-time BM had a synergistic effect on the enzymatic digestibility of Japanese cypress.
- Ohtsuki T, Noda S, Ui S (2011) Improvement of bioconversion suitability of Japanese cypress wood by combination of UV radiation, ozonization and decay treatment with white-rot and brown-rot fungi. Can J Pure Appl Sci 5:1333–1343Google Scholar
- Tappi Press (1992) Tappi Test Methods, Method T249 cm-85, Atlanta, GAGoogle Scholar
- Wu S, Agryropoulos DS (2003) An improved method for isolating lignin in high yield and purity. J Pulp Paper Sci 29:235–240Google Scholar
- Yanagida T, Fujimoto S, Hideno A, Inoue H, Tsukahara K, Sawayama S, Minowa T (2009) Energy and economic evaluation for ethanol production of non sulfuric acid pretreatment method from rice straw. J Jap Soc Energy Resour 30:8–14Google Scholar