Wood Science and Technology

, Volume 47, Issue 2, pp 381–393 | Cite as

Effect of alcohol-based organosolv treatment combined with short-time ball milling on the enzymatic hydrolysis of Japanese cypress (Chamaecyparis obtusa)

  • Akihiro Hideno
  • Ayato Kawashima
  • Masayoshi Fukuoka
  • Takashi Endo
  • Katsuhisa Honda
  • Masatoshi Morita
Original

Abstract

Japanese cypress (Chamaecyparis obtusa) is a recalcitrance softwood widely used as building material in Japan. A combined technique of alcohol-based organosolv treatment and ball milling (BM) of this softwood for the production of fermentable sugars from lignocellulose was investigated. Ethanol, ethylene glycol, water, and their mixtures were used as solvents, and acetic acid was used as a proton donor. After solvolysis, a residual sample was subjected to short-time BM, and the entire residue was evaluated by saccharification with Acremonium cellulase. Short-time BM after alcohol-based organosolv treatment greatly improved the enzymatic digestibility and decreased the required severity of organosolv treatment. Moreover, alcohol-based organosolv treatment increased the efficiency and reduced the time required for BM despite small quantity of removed lignin. It was found that the combination of alcohol-based organosolv treatment in mild condition and short-time BM had a synergistic effect on the enzymatic digestibility of Japanese cypress.

References

  1. Ahmed S, Capart R (2002) Liquefaction of wood in two successive steps: solvolysis in ethylene-glycol and catalytic hydrotreatment. Appl Energy 72:631–644CrossRefGoogle Scholar
  2. Alviraa P, Pejóa ET, Ballesterosa M, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 101:4851–4861CrossRefGoogle Scholar
  3. Chun HL, Johnson DK, Black SK (1990) Organosolv pretreatment for enzymatic hydrolysis of poplars. 2. Catalyst effects and the combined severity parameter. Ind Eng Chem Res 29:156–162CrossRefGoogle Scholar
  4. Guerra A, Filpponen I, Lucia LA, Saquing C, Baumberger S, Agryropoulos DS (2006a) Toward a better understanding of the lignin isolation process from wood. J Agric Food Chem 54:5939–5947PubMedCrossRefGoogle Scholar
  5. Guerra A, Filpponen I, Lucia LA, Agryropoulos DS (2006b) Comparative evaluation of three lignin isolation protocols for various wood species. J Agric Food Chem 54:9696–9705PubMedCrossRefGoogle Scholar
  6. Hägerdala BH, Galbea M, Grauslunda MFG, Lidéna G, Zacchia G (2006) Bio-ethanol—the fuel of tomorrow from the residues of today. Trends Biotechnol 24:549–556CrossRefGoogle Scholar
  7. Hallac BB, Sannigrahi P, Pu Y, Ray M, Murphy RJ, Ragauskas AJ (2010) Effect of ethanol organosolv pretreatment on enzymatic hydrolysis of Buddleja davidii stem biomass. Ind Eng Chem Res 49:1467–1472CrossRefGoogle Scholar
  8. Hendriks ATWM, Zeeman G (2008) Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresour Technol 100:10–18PubMedCrossRefGoogle Scholar
  9. Hideno A, Inoue H, Tsukahara K, Fujimoto S, Minowa T, Inoue S, Endo T, Sawayama S (2009) Wet disk milling pretreatment without sulfuric acid for enzymatic hydrolysis of rice straw. Bioresour Technol 100:2706–2711PubMedCrossRefGoogle Scholar
  10. Igarashi K, Koivula A, Wada M, Kimura S, Penttila M, Samejima M (2009) High speed atomic force microscopy visualizes processive movement of Trichoderma reesei cellobiohydrolase 1 on crystalline cellulose. J Biol Chem 25:36186–36190CrossRefGoogle Scholar
  11. Inoue H, Yano S, Endo T, Sasaki T, Sawayama S (2008) Combining hot compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus. Biotechnol Biofuels 1:2PubMedCrossRefGoogle Scholar
  12. Lee I, Evans B, Woodward J (2000) The mechanism of cellulase action on cotton fibers: evidence from atomic force microscopy. Ultramicroscopy 82:213–221PubMedCrossRefGoogle Scholar
  13. Maekawa E (1996) On an available pretreatment for the enzymatic saccharification of lignocellulosic materials. Wood Sci Technol 30:133–139CrossRefGoogle Scholar
  14. Ohtsuki T, Noda S, Ui S (2011) Improvement of bioconversion suitability of Japanese cypress wood by combination of UV radiation, ozonization and decay treatment with white-rot and brown-rot fungi. Can J Pure Appl Sci 5:1333–1343Google Scholar
  15. Okuda N, Ninomiya K, Takao M, Katakura Y, Shioya S (2007) Microaeration enhances productivity of bioethanol from hydrolysate of waste house wood using ethanologenic Escherichia coli KO11. J Biosci Bioeng 103:350–357PubMedCrossRefGoogle Scholar
  16. Overend RP, Chornet E, Gascoigne A (1987) Fraction of lignocellulosics by steam-aqueous pretreatments [and discussion]. Philos Trans R Soc Lond A 321:523–526CrossRefGoogle Scholar
  17. Papatheofanousa MG, Billaa E, Koullasa DP, Montiesb B, Koukios EG (1995) Two-stage acid-catalyzed fractionation of lignocellulosic biomass in aqueous ethanol systems at low temperatures. Bioresour Technol 54:305–310CrossRefGoogle Scholar
  18. Sun F, Chen H (2008) Enhanced enzymatic hydrolysis of wheat straw by aqueous glycerol pretreatment. Bioresour Technol 99:6156–6161PubMedCrossRefGoogle Scholar
  19. Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1–11PubMedCrossRefGoogle Scholar
  20. Tappi Press (1992) Tappi Test Methods, Method T249 cm-85, Atlanta, GAGoogle Scholar
  21. Teramoto Y, Tanaka N, Lee SH, Endo T (2007) Pretreatment of Eucalyptus wood chips for enzymatic saccharification using combined sulfuric acid-free ethanol cooking and ball milling. Biotechnol Bioeng 99:75–85CrossRefGoogle Scholar
  22. Teramoto Y, Lee SH, Endo T (2008) Pretreatment of woody and herbaceous biomass for enzymatic saccharification using sulfuric acid-free ethanol cooking. Bioresour Technol 99:8856–8863PubMedCrossRefGoogle Scholar
  23. Vanasse C, Lemonnier JP, Eugene D, Chornet E (1988) Pretreatment of wood flour slurries prior to liquefaction. Can J Chem Eng 66:107–111CrossRefGoogle Scholar
  24. Wu S, Agryropoulos DS (2003) An improved method for isolating lignin in high yield and purity. J Pulp Paper Sci 29:235–240Google Scholar
  25. Yamada T, Ono H (2001) Characterization of the products resulting from ethylene glycol liquefaction of cellulose. J Jap Wood Res Soc 47:458–464CrossRefGoogle Scholar
  26. Yanagida T, Fujimoto S, Hideno A, Inoue H, Tsukahara K, Sawayama S, Minowa T (2009) Energy and economic evaluation for ethanol production of non sulfuric acid pretreatment method from rice straw. J Jap Soc Energy Resour 30:8–14Google Scholar
  27. Zhang T, Zhou Y, Liu D, Petrus L (2007) Qualitative analysis of products formed during the acid catalyzed liquefaction of bagasse in ethylene glycol. Bioresour Technol 98:1454–1459PubMedCrossRefGoogle Scholar
  28. Zhao X, Cheng K, Liu D (2009) Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis. Appl Microbiol Biotechnol 82:815–827PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Akihiro Hideno
    • 1
  • Ayato Kawashima
    • 1
  • Masayoshi Fukuoka
    • 1
  • Takashi Endo
    • 2
  • Katsuhisa Honda
    • 1
  • Masatoshi Morita
    • 1
  1. 1.Ehime UniversityMatsuyamaJapan
  2. 2.Biomass Technology Research CenterNational Institute of Advanced Industrial Science and Technology (AIST)Higashi-HiroshimaJapan

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