Wood Science and Technology

, Volume 43, Issue 1–2, pp 85–95 | Cite as

Antibacterial and antifungal activities of Myracrodruon urundeuva heartwood

  • Roberto A. Sá
  • Francis S. Gomes
  • Thiago H. Napoleão
  • Nataly D. L. Santos
  • Carla M. L. Melo
  • Norma B. Gusmão
  • Luana C. B. B. Coelho
  • Patrícia M. G. Paiva
  • Lothar W. Bieber
Original

Abstract

The aim of this work was to isolate a lectin from Myracrodruon urundeuva heartwood and to evaluate its antimicrobial activity against bacteria and fungi that attack plants, including woods. The lectin was isolated from heartwood through affinity chromatography on a chitin column monitored by hemagglutination assay. The lectin inhibited Gram-negative and Gram-positive bacteria and was more effective than antifungal Cercobin in growth inhibition of phytopathogenic fungi. The detected antimicrobial activity reveals the possible role of the lectin in the resistance of M. urundeuva heartwood against deteriorative biological agents. The M. urundeuva lectin is the first bioactive peptide found in heartwood, probably stored as a chemical protection against biodegradation.

Keywords

Minimal Inhibitory Concentration Fusarium Antifungal Activity Colony Form Unit Ammonium Sulphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CFU

Colony forming units

F1

40–60% fraction

HA

Hemagglutinating activity

MAC

Minimal agglutinating concentration

MBC

Minimal bactericide concentration

MIC

Minimal inhibitory concentration

NA

Nutrient Agar medium

NB

Nutrient Broth medium

SHA

Specific hemagglutinating activity

YNB

Yeast nitrogen base medium

Notes

Acknowledgments

The authors express their gratitude to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for research grants. Authors are deeply grateful to Maria Barbosa Reis da Silva (for the technical assistance) and to Msc. Gonçalo Mendes da Conceição (for the identification of the botanical material).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Roberto A. Sá
    • 1
  • Francis S. Gomes
    • 2
  • Thiago H. Napoleão
    • 2
  • Nataly D. L. Santos
    • 2
  • Carla M. L. Melo
    • 3
  • Norma B. Gusmão
    • 3
  • Luana C. B. B. Coelho
    • 2
  • Patrícia M. G. Paiva
    • 2
  • Lothar W. Bieber
    • 1
  1. 1.Departamento de Química Fundamental, CCENUniversidade Federal de PernambucoRecifeBrazil
  2. 2.Departamento de Bioquímica, CCBUniversidade Federal de PernambucoRecifeBrazil
  3. 3.Departamento de Antibióticos, CCBUniversidade Federal de PernambucoRecifeBrazil

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