Advertisement

Calcified Tissue International

, Volume 64, Issue 3, pp 219–223 | Cite as

Etidronate (EHDP) Inhibits Osteoclastic-Bone Resorption, Promotes Apoptosis and Disrupts Actin Rings in Isolate-Mature Osteoclasts

  • E.  Hiroi-Furuya
  • T.  Kameda
  • K.  Hiura
  • H.  Mano
  • K.  Miyazawa
  • Y.  Nakamaru
  • M.  Watanabe-Mano
  • N.  Okuda
  • J.  Shimada
  • Y.  Yamamoto
  • Y.  Hakeda
  • M.  Kumegawa

Abstract.

Bisphosphonates, therapeutic reagents against tumoral bone diseases (Paget's disease or osteoporosis), are potent inhibitors of bone resorption. The mechanisms by which they directly act on mature osteoclasts remain unclear. Using a recently developed technique for isolation of highly purified mammalian mature osteoclasts, we demonstrated that etidronate [ethane-1-hydroxy-1,1-diphosphonate (EHDP), 1-hydroxy-1,1-ethylidenebisphosphonate], inhibited directly osteoclastic bone-resorbing activity by pit assay. In addition, EHDP also directly induced apoptosis and disrupted actin rings in osteoclasts. The data support previous data on non-purified osteoclasts and results in vivo.

Key words: EHDP — Osteoclast — Apoptosis — Actin rings. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • E.  Hiroi-Furuya
    • 1
  • T.  Kameda
    • 1
  • K.  Hiura
    • 2
  • H.  Mano
    • 2
  • K.  Miyazawa
    • 2
  • Y.  Nakamaru
    • 2
  • M.  Watanabe-Mano
    • 2
  • N.  Okuda
    • 2
  • J.  Shimada
    • 3
  • Y.  Yamamoto
    • 3
  • Y.  Hakeda
    • 3
  • M.  Kumegawa
    • 3
  1. 1.Department of Oral Anatomy, Meikai University School of Dentistry, Sakado, Saitama 350-02, JapanJP
  2. 2.Department of Orthodontics, Tokushima University School of Dentistry, Tokushima, Tokushima 770, JapanJP
  3. 3.First Department of Oral and Maxillofacial Surgery, Meikai University School of Dentistry, Sakado, Saitama 350-02, JapanJP

Personalised recommendations