Calcified Tissue International

, Volume 66, Issue 5, pp 388–396

Bone Sialoprotein (BSP) is a Crucial Factor for the Expression of Osteoblastic Phenotypes of Bone Marrow Cells Cultured on Type I Collagen Matrix

  • M.  Mizuno
  • T.  Imai
  • R.  Fujisawa
  • H.  Tani
  • Y.  Kuboki
Article

DOI: 10.1007/s002230010078

Cite this article as:
Mizuno, M., Imai, T., Fujisawa, R. et al. Calcif Tissue Int (2000) 66: 388. doi:10.1007/s002230010078

Abstract

In this study, we demonstrated that type I collagen matrix induced the expression of osteoblastic phenotypes of bone marrow cells, and that antibone sialoprotein (BSP) monoclonal antibody suppressed the expression of these phenotypes. On the other hand, BSP accelerated the expression of osteoblastic phenotypes of bone marrow cells. The adherent bone marrow cells were harvested from rat femur and cultured on type I collagen matrix gels in medium containing 15% fetal calf serum, neither β-glycerophosphate nor glucocorticoid. Cells showed osteoblastic phenotypes (high alkaline phosphatase activity, osteocalcin synthesis, and responsiveness against parathyroid hormone) on collagen matrix gels at week 3 after the inoculation, and simultaneously, BSP was detected in the conditioned medium by Western blotting using an anti-BSP monoclonal antibody. However, cells in the conventional culture dishes did not show osteoblastic phenotypes during the experimental period. To investigate the physiological function of BSP in osteoblastic differentiation, bone marrow cells were cultured on collagen matrix with an anti-BSP monoclonal antibody for 3 weeks. This treatment suppressed the expression of the osteoblastic phenotypes, and the effect of the antibody was abolished by the addition of bovine bone BSP. Furthermore, bovine bone BSP stimulated the expression of osteoblastic phenotypes of bone marrow cells. Our results indicate that BSP plays a crucial role in the expression of osteoblastic phenotypes of bone marrow cells.

Key words: Bone sialoprotein — Osteoblasts — Bone marrow cells. 

Copyright information

© Springer-Verlag New York 2000

Authors and Affiliations

  • M.  Mizuno
    • 1
  • T.  Imai
    • 2
  • R.  Fujisawa
    • 1
  • H.  Tani
    • 2
  • Y.  Kuboki
    • 1
  1. 1.Department of Biochemistry, School of DentistryHokkaido UniversitySapporoJapan
  2. 2.Department of Preventive Dentistry, School of DentistryHokkaido UniversitySapporoJapan

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