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Calcified Tissue International

, Volume 103, Issue 1, pp 80–94 | Cite as

The Advantages of Bilateral Osteotomy Over Unilateral Osteotomy for Osteoporotic Bone Healing

  • M. KomrakovaEmail author
  • J. Fiebig
  • D. B. Hoffmann
  • C. Krischek
  • W. Lehmann
  • K. M. Stuermer
  • S. Sehmisch
Original Research
  • 129 Downloads

Abstract

Most models of osteoporotic bone fractures are performed unilaterally (UL). We investigated healing of tibia osteotomy performed either UL or bilaterally (BL) in ovariectomized rats. Behavior of animals and muscle structure were assessed. Three-month-old female Sprague-Dawley rats were ovariectomized (n = 32). After 10 weeks, half the rats underwent UL osteotomy of tibia metaphysis (right limb) with plate osteosynthesis. The other rats were osteotomized BL. Half of the rats in each group received either standard pain treatment with carprofen (5 mg/kg body weight (BW), 1x/day for 2 days) or carprofen and buprenorphine (5 mg/kg BW, 1x/day and 0.03 mg/kg BW, 2x/day for 5 days) after osteotomy. The UL rats started to load the injured limb from day 27 ± 9; BL rats did this from day 4 ± 4 onward. The UL rats more frequently loaded only one hind limb; BL rats more often loaded both hind limbs. Osteotomy was not bridged in 20% of UL rats and in 4% of BL rats. Callus volume and bone volume fraction were lower in UL group. Weight and fiber size of UL-intact limb muscles were enhanced, compared to the osteotomized limb and those in BL group. Most of the other parameters which assess physiology, activity, body posture, head, or coat were not different. The effect of two pain therapies was not significant on any variable studied. Welfare of the animals was acceptable in all rats. In UL rats, bone healing was delayed. The more advanced healing in BL rats suggested a positive effect of earlier loading. In studies on bone healing, it is advisable to perform BL osteotomy.

Keywords

Bone fracture model Bilateral Unilateral Osteotomy Ovariectomy Rat 

Notes

Acknowledgements

The authors are grateful to their colleagues, R. Castro-Machguth, A. Witt, and R. Wigger, for technical support. We are thankful to Dr. V. Reupke for help in preparing a score sheet. We would like to acknowledge the important role of Herr Dr. T. Rack in the developing of bone analyses in the last decade.

Compliance with Ethical Standards

Conflict of interest

M Komrakova, J Fiebig, D Hoffmann, C Krischek, W Lehmann, KM Stuermer, and S Sehmisch declare that they have no conflict of interest.

Human and Animal Rights and Informed Consent

The animal study protocol was approved by the local regional government (33.4-42502-04-14/1396, Oldenburg, Germany) in accordance with German animal protection laws prior to performing the study.

Supplementary material

223_2018_392_MOESM1_ESM.pptx (7.7 mb)
Supplementary material 1 (PPTX 7930 KB)
223_2018_392_MOESM2_ESM.pptx (1.6 mb)
Supplementary material 2 (PPTX 1587 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Trauma Surgery, Orthopaedics and Plastic SurgeryUniversity Medical CenterGoettingenGermany
  2. 2.Department of Animal SciencesUniversity of GoettingenGoettingenGermany
  3. 3.Institute of Food Quality and SafetyFoundation University of Veterinary MedicineHanoverGermany

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