The Advantages of Bilateral Osteotomy Over Unilateral Osteotomy for Osteoporotic Bone Healing
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Most models of osteoporotic bone fractures are performed unilaterally (UL). We investigated healing of tibia osteotomy performed either UL or bilaterally (BL) in ovariectomized rats. Behavior of animals and muscle structure were assessed. Three-month-old female Sprague-Dawley rats were ovariectomized (n = 32). After 10 weeks, half the rats underwent UL osteotomy of tibia metaphysis (right limb) with plate osteosynthesis. The other rats were osteotomized BL. Half of the rats in each group received either standard pain treatment with carprofen (5 mg/kg body weight (BW), 1x/day for 2 days) or carprofen and buprenorphine (5 mg/kg BW, 1x/day and 0.03 mg/kg BW, 2x/day for 5 days) after osteotomy. The UL rats started to load the injured limb from day 27 ± 9; BL rats did this from day 4 ± 4 onward. The UL rats more frequently loaded only one hind limb; BL rats more often loaded both hind limbs. Osteotomy was not bridged in 20% of UL rats and in 4% of BL rats. Callus volume and bone volume fraction were lower in UL group. Weight and fiber size of UL-intact limb muscles were enhanced, compared to the osteotomized limb and those in BL group. Most of the other parameters which assess physiology, activity, body posture, head, or coat were not different. The effect of two pain therapies was not significant on any variable studied. Welfare of the animals was acceptable in all rats. In UL rats, bone healing was delayed. The more advanced healing in BL rats suggested a positive effect of earlier loading. In studies on bone healing, it is advisable to perform BL osteotomy.
KeywordsBone fracture model Bilateral Unilateral Osteotomy Ovariectomy Rat
The authors are grateful to their colleagues, R. Castro-Machguth, A. Witt, and R. Wigger, for technical support. We are thankful to Dr. V. Reupke for help in preparing a score sheet. We would like to acknowledge the important role of Herr Dr. T. Rack in the developing of bone analyses in the last decade.
Compliance with Ethical Standards
Conflict of interest
M Komrakova, J Fiebig, D Hoffmann, C Krischek, W Lehmann, KM Stuermer, and S Sehmisch declare that they have no conflict of interest.
Human and Animal Rights and Informed Consent
The animal study protocol was approved by the local regional government (33.4-42502-04-14/1396, Oldenburg, Germany) in accordance with German animal protection laws prior to performing the study.
- 18.Hicks DA (2013) Kinetic and kinematic evaluation of compensatory movements of the head, pelvis and thoraco-lumbar spine associated with asymmetrical weight bearing of the pelvic limbs in dogs. PhD diss., University of Tennessee, 2013. http://trace.tennessee.edu/utk_graddiss/1734
- 20.Rueff-Barroso CR, Milagres D, do Valle J, Casimiro-Lopes G, Nogueira-Neto JF, Zanier JF et al (2008) Bone healing in rats submitted to weight-bearing and non-weight-bearing exercises. Med Sci Monit 14:BR231-236Google Scholar
- 22.Russell WMS, Burch RL (1959) The principles of humane experimental technique. Methuen, LondonGoogle Scholar
- 24.Rahn DA (1976) The fluorochrome sequence labelling of the bone. Nova Acta Leopold 44:249–255Google Scholar
- 32.Stein KH, Flenker H, Storjohann H (1998) Basiswissen Histologie und Zytologie. Umschau-Zeitschrift-Verlag, Breidenstein GmbH, Frankfurt am Main, PublisherGoogle Scholar
- 33.Horak V (1983) A successive histochemical staining for succinate dehydrogenase and ‘‘reversed’’—ATPase in a single section for the skeletal muscle fibre typing. Histochem Cell Biol 78:545–553Google Scholar
- 45.Madaro L, Smeriglio P, Molinaro M, Bouché M (2008) Unilateral immobilization: a simple model of limb atrophy in mice. Basic Applied Myology 18:149–153Google Scholar
- 54.Jaffe DE, Yoo D, Blevins J, Gasbarro G, Hughes T, Schultz B, Stains J, Pellegrini VD (2012) Fracture fixation determines the pathway of osseous repair: a bilateral femur fracture model in a rat. Poster No. 0440, ORS 2012 Annual Meeting https://www.ors.org/Transactions/58/0440.pdf