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Impact of Chiropractic Manipulation on Bone and Skeletal Muscle of Ovariectomized Rats

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Abstract

Evidence suggests that chiropractic manipulation might exert positive effects in osteoporotic patients. The aim of this study was to evaluate the effects of chiropractic manipulation on bone structure and skeletal muscle in rats with bone loss caused by ovariectomy (OVX). The 6-month old Sprague-Dawley rats at 10 weeks following OVX or sham operation (Sh) did not suffer chiropractic manipulation (NM group) or were submitted to true chiropractic manipulation using the chiropractic adjusting instrument Activator V® three times/week for 6 weeks as follows: Force 1 setting was applied onto the tibial tubercle of the rat right hind limb (TM group), whereas the corresponding left hind limb received a false manipulation (FM group) consisting of ActivatorV® firing in the air and slightly touching the tibial tubercle. Bone mineral density (BMD) and bone mineral content (BMC) were determined in long bones and L3–L4 vertebrae in all rats. Femora and tibia were analyzed by μCT. Mechano growth factor (MGF) was detected in long bones and soleus, quadriceps and tibial muscles by immunohistochemistry and Western blot. The decrease of BMD and BMC as well as trabecular bone impairment in the long bones of OVX rats vs Sh controls was partially reversed in the TM group versus FM or NM rats. This bone improvement by chiropractic manipulation was associated with an increased MGF expression in the quadriceps and the anterior tibial muscle in OVX rats. These findings support the notion that chiropractic manipulation can ameliorate osteoporotic bone at least partly by targeting skeletal muscle.

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Acknowledgements

This work was supported in part by grants from the Spanish Chiropractic Association (AEQ) and Fondation de Recherche Chiropratique du Québec (Québec, Canada).

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Authors and Affiliations

  1. Madrid College of Chiropractic, Real Centro Universitario Escorial-María Cristina, Paseo de los Alamillos, 2, San Lorenzo de El Escorial, 28200, Madrid, Spain

    A. López-Herradón, R. Fujikawa, M. Gómez-Marín, J. P. Stedile-Lovatel, P. Ruiz, I. Muñoz & A. Ortega-de Mues

  2. Laboratorio de Metabolismo Mineral y Óseo, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz-UAM, Madrid, Spain

    A. López-Herradón, J. A. Ardura & P. Esbrit

  3. Unidad de Imagen Molecular, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain

    F. Mulero

  4. Unidad de Epidemiología y Bioestadística, IIS-Fundación Jiménez Díaz-UAM, Madrid, Spain

    I. Mahíllo-Fernández

Authors
  1. A. López-Herradón
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  2. R. Fujikawa
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  3. M. Gómez-Marín
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  4. J. P. Stedile-Lovatel
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  6. J. A. Ardura
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  7. P. Ruiz
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  8. I. Muñoz
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  9. P. Esbrit
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  10. I. Mahíllo-Fernández
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  11. A. Ortega-de Mues
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Corresponding author

Correspondence to A. Ortega-de Mues.

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Conflict of interest

A. López-Herradón, R. Fujikawa, M. Gómez-Marín, J. P. Stedile-Lovatel, F. Mulero, J. A. Ardura, P. Ruiz, I. Muñoz, P. Esbrit, I. Mahíllo-Fernández and A.Ortega-de Mues declare that they do not have conflict of interest.

Ethical Approval

All animal procedures were carried out according to the guidelines of European Union directives (2010/63/EU). All experimental protocols were approved by the Institutional Animal Care and Use Committee at Fundación Jiménez Díaz.

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López-Herradón, A., Fujikawa, R., Gómez-Marín, M. et al. Impact of Chiropractic Manipulation on Bone and Skeletal Muscle of Ovariectomized Rats. Calcif Tissue Int 101, 519–529 (2017). https://doi.org/10.1007/s00223-017-0304-1

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  • DOI: https://doi.org/10.1007/s00223-017-0304-1

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