The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats
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The aim of the present study was to study the effect of combined therapy of teriparatide (PTH) or strontium ranelate (SR) with whole-body vibration (WBV) on bone healing and muscle properties in an osteopenic rat model. Seventy-two rats (3 months old) were bilaterally ovariectomized (Ovx), and 12 rats were left intact (Non-Ovx). After 8 weeks, bilateral transverse osteotomy was performed at the tibia metaphysis in all rats. Thereafter, Ovx rats were divided into six groups (n = 12): (1) Ovx—no treatment, (2) Ovx + vibration (Vib), (3) SR, (4) SR + Vib, (5) PTH, and (6) PTH + Vib. PTH (40 μg/kg BW sc. 5×/week) and SR (613 mg/kg BW in food daily) were applied on the day of ovariectomy, vibration treatments 5 days later (vertical, 70 Hz, 0.5 mm, 2×/day for 15 min) for up to 6 weeks. In the WBV + SR group, the callus density, trabecular number, and Alp and Oc gene expression were decreased compared to SR alone. In the WBV + PTH group, the cortical and callus widths, biomechanical properties, Opg gene expression, and Opg/Rankl ratio were increased; the cortical and callus densities were decreased compared to PTH alone. A case of non-bridging was found in both vibrated groups. Vibration alone did not change the bone parameters; PTH possessed a stronger effect than SR therapy. In muscles, combined therapies improved the fiber size of Ovx rats. WBV could be applied alone or in combination with anti-osteoporosis drug therapy to improve muscle tissue. However, in patients with fractures, anti-osteoporosis treatments and the application of vibration could have an adverse effect on bone healing.
KeywordsWhole-body vibration Teriparatide Strontium ranelate Combined therapy Bone healing Muscle structure
This study was supported by the German Research Foundation (DFG, SE 1966/5-1). The authors are grateful to their colleagues R. Castro-Machguth, A. Witt, and R. Wigger for their technical support.
Compliance with Ethical Standards
Conflict of interest
M. Komrakova, D. B. Hoffmann, V. Nuehnen, H. Stueber, M. Wassmann, M. Wicke, M. Tezval, K. M. Stuermer, and S. Sehmisch declare that they have no conflict of interest.
Human and Animal Rights and Informed Consent
The animal study protocol was approved by the local regional government (33.9-42502-04-12/0854, Oldendurg, Germany) in accordance with German animal protection laws prior to performing the study.
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