Calcified Tissue International

, Volume 90, Issue 4, pp 251–262 | Cite as

Changes in Parameters of Bone Metabolism in Postmenopausal Women Following a 12-Month Intervention Period Using Dairy Products Enriched with Calcium, Vitamin D, and Phylloquinone (Vitamin K1) or Menaquinone-7 (Vitamin K2): The Postmenopausal Health Study II

  • Spyridon Kanellakis
  • George Moschonis
  • Roxane Tenta
  • Anne Schaafsma
  • Ellen G. H. M. van den Heuvel
  • Nikolaos Papaioannou
  • George Lyritis
  • Yannis ManiosEmail author
Original Research


The objective of the present study was to examine the effect of dairy products enriched with calcium, vitamin D3, and phylloquinone (vitamin K1) or menaquinone-7 (vitamin K2) on parameters of bone metabolism in postmenopausal women following a 12-month intervention. Postmenopausal women were divided into three intervention groups and a control group (CG). All three intervention groups attended biweekly sessions and received fortified dairy products providing daily 800 mg of calcium and 10 μg of vitamin D3 (CaD). Furthermore, in two of the three intervention groups the dairy products were also enriched with vitamin K, providing daily 100 μg of either phylloquinone (CaDK1) or menaquinone-7 (CaDK2). The increase observed for serum 25(OH)D levels in all intervention groups and the increase observed for serum IGF-I levels in the CaDK2 group differed significantly compared to the changes observed in CG (P = 0.010 and P = 0.028, respectively). Furthermore, both the CaDK1 and CaDK2 groups had a significantly lower mean serum undercarboxylated osteocalcin to osteocalcin ratio and urine deoxypyridinoline levels at follow-up compared to the CaD and CG groups (P = 0.001 and P = 0.047, respectively). Significant increases in total-body BMD were observed in all intervention groups compared to CG (P < 0.05), while significant increases in lumbar spine BMD were observed only for CaDK1 and CaDK2 compared to CG (P < 0.05) after controlling for changes in serum 25(OH)D levels and dietary calcium intake. In conclusion, the present study revealed more favorable changes in bone metabolism and bone mass indices for the two vitamin K-supplemented groups, mainly reflected in the suppression of serum levels of bone remodeling indices and in the more positive changes in lumbar spine BMD for these two study groups.


Vitamin K Vitamin D Calcium Bone metabolism 



This work was supported by a research grant from FrieslandCampina. The study sponsor had no role in the collection and analysis of the data, or the submission of the report. The authors thank Anastasia Vandorou, Katerina Kyriakou, Vasiliki Dede, Odysseas Androutsos, Konstantina Maragkopoulou, Efstathoula Argyri, Angeliki Giannopoulou, Vassiliki Iatridi, Christina Maurogianni, Sotiria Pitsikou, Sofia Chondrou, George Saltaouras, and Dimitra Meladaki (dietitians) for their valuable contribution in data collection and processing.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Spyridon Kanellakis
    • 1
  • George Moschonis
    • 1
  • Roxane Tenta
    • 1
  • Anne Schaafsma
    • 2
  • Ellen G. H. M. van den Heuvel
    • 2
  • Nikolaos Papaioannou
    • 3
  • George Lyritis
    • 3
  • Yannis Manios
    • 1
    Email author
  1. 1.Department of Nutrition and DieteticsHarokopio UniversityAthensGreece
  2. 2.FrieslandCampinaAmersfoortThe Netherlands
  3. 3.Laboratory for the Research of the Musculoskeletal System, School of MedicineUniversity of AthensAthensGreece

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