Calcified Tissue International

, Volume 86, Issue 4, pp 325–332

β1 Integrins Mediate Mechanosensitive Signaling Pathways in Osteocytes

  • Julie B. Litzenberger
  • Jae-Beom Kim
  • Padmaja Tummala
  • Christopher R. Jacobs
Article

DOI: 10.1007/s00223-010-9343-6

Cite this article as:
Litzenberger, J.B., Kim, JB., Tummala, P. et al. Calcif Tissue Int (2010) 86: 325. doi:10.1007/s00223-010-9343-6

Abstract

Integrins are cell-substrate adhesion proteins that initiate intracellular signaling and may serve as mechanosensors in bone. MLO-Y4 cells were stably transfected with a dominant negative form of the β1 integrin subunit (β1DN) containing the transmembrane domain and cytoplasmic tail of β1 integrin. Cells expressing β1DN had reduced vinculin localization to focal contacts but no change in intracellular actin organization. When exposed to oscillatory fluid flow, β1DN cells exhibited a significant reduction in the upregulation of cyclooxygenase-2 gene expression and prostaglandin E2 release. Similarly, the ratio of receptor activator of NF-κB ligand mRNA to osteoprotegerin mRNA decreased significantly after exposure to fluid flow in control cells but not in β1DN cells. Interfering with integrin signaling did not affect mechanically induced intracellular calcium mobilization. These data suggest that integrins may initiate the cellular response of osteocytes to dynamic fluid flow and may serve as mechanosensitive molecules in bone.

Keywords

Integrin Mechanotransduction Osteocyte Fluid flow Bone 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Julie B. Litzenberger
    • 1
    • 2
  • Jae-Beom Kim
    • 3
    • 4
  • Padmaja Tummala
    • 1
    • 5
  • Christopher R. Jacobs
    • 6
  1. 1.Department of Veterans AffairsBone and Joint Rehabilitation CenterPalo AltoUSA
  2. 2.Department of BioengineeringStanford UniversityStanfordUSA
  3. 3.Department of StomatologyUniversity of CaliforniaStanfordUSA
  4. 4.Caliper Life SciencesAlamedaUSA
  5. 5.MedImmune VaccinesMountain ViewUSA
  6. 6.Biomedical Engineering DepartmentColumbia UniversityNew YorkUSA

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