Calcified Tissue International

, Volume 82, Issue 2, pp 162–169 | Cite as

Correlation between Hydroxyapatite Crystallite Orientation and Ultrasonic Wave Velocities in Bovine Cortical Bone

  • Yu Yamato
  • Mami Matsukawa
  • Takahiko Yanagitani
  • Kaoru Yamazaki
  • Hirofumi Mizukawa
  • Akira Nagano
Article

Abstract

The mineral component of bone is mainly composed of calcium phosphate, constituting 70% of total bone mass almost entirely in the form of hydroxyapatite (HAp) crystals. HAp crystals have a hexagonal system and uniaxial elastic anisotropy. The objective of this study was to investigate the effect of HAp crystallite preference on macroscopic elasticity. Ultrasonic longitudinal wave velocity and the orientation of HAp crystallites in bovine cortical bone are discussed, considering microstructure, density, and bone mineral density (BMD). Eighty cube samples of cortical bone were made from two bovine femurs. The orientation of HAp crystallites was evaluated by integrated intensity ratio of (0002) peak using an X-ray diffractometer. Ultrasonic longitudinal wave velocity was investigated with a conventional pulse system. The intensity ratio of HAp crystallites and velocity were measured in three orthogonal directions; most HAp crystallites aligned in the axial direction of the femurs. Our results demonstrate a linear correlation between velocity and intensity ratio in the axial direction. Significant correlation between velocity and BMD values was observed; however, the correlation disappeared if we focused on the identical type of microstructure. In conclusion, differences in microstructure type have an impact on density and BMD, which clearly affects the velocity. In addition, at the nanoscopic level, HAp crystallites aligned in the axial direction also affected the velocity and anisotropy.

Keywords

Bone density technology Ultrasound Crystal structure/crystallinity Bone histology/histomorphometry Bone architecture/structure 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yu Yamato
    • 1
  • Mami Matsukawa
    • 2
  • Takahiko Yanagitani
    • 2
  • Kaoru Yamazaki
    • 1
  • Hirofumi Mizukawa
    • 2
  • Akira Nagano
    • 1
  1. 1.Department of Orthopaedic SurgeryHamamatsu University School of MedicineHamamatsuJapan
  2. 2.Faculty of EngineeringDoshisha UniversityKyotoJapan

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