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Calcified Tissue International

, Volume 80, Issue 4, pp 233–243 | Cite as

Serum from Postmenopausal Women Directs Differentiation of Human Clonal Osteoprogenitor Cells from an Osteoblastic toward an Adipocytic Phenotype

  • Bradley StringerEmail author
  • Rachel Waddington
  • Alastair Sloan
  • Adam Houghton
  • Mike Stone
  • Graham Russell
  • George Foster
Article

Abstract

A consistent observation in osteoporosis is bone volume reduction accompanied by increased marrow adipose tissue. No single cause linking the two phenomena has yet been identified. In a human progenitor cell clone (hOP 7) derived from bone marrow, however, we have demonstrated that rabbit serum can direct differentiation away from an osteoblast lineage to one of adipocytes. We now report on whether human serum has a similar effect. Serum was collected from 10 pre- and 10 postmenopausal women and from the 10 postmenopausal women before and following 6-week hormone replacement therapy (HRT). hOP 7 cells were cultured with the various sera, and after 7-14 days adipocytogenesis was determined by oil red O staining and lipoprotein lipase (LPL) and glycerol 3-phosphate dehydrogenase (G3PDH) expression. Incubation with 10% premenopausal serum led to labeling of 10.9% of cells (P < 0.05) with oil red O, whereas application of 10% postmenopausal serum led to a much larger effect, 43.5% labeling (P < 0.001 with respect to premenopausal serum). Oil red O positivity was accompanied by loss of type I collagen expression and increased LPL and G3PDH expression. HRT did not reverse the adipocytogenic effect of postmenopausal serum. In conclusion, serum from postmenopausal women contains factors that steer hOP 7 bone progenitor cells toward an adipocytic phenotype, irrespective of HRT. The study suggests a role for serum factors in the development of fatty marrow in postmenopausal osteoporosis.

Keywords

Adipocyte Differentiation Menopause Osteoblast Serum 

Notes

Acknowledgment

We are grateful to the Arthritis Research Campaign, the Nuffield Foundation, the University of Sheffield, and Cardiff University for supporting this work.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Bradley Stringer
    • 1
    Email author
  • Rachel Waddington
    • 2
  • Alastair Sloan
    • 1
  • Adam Houghton
    • 3
  • Mike Stone
    • 4
  • Graham Russell
    • 5
  • George Foster
    • 6
  1. 1.Department of Oral Surgery, Medicine and PathologyDental School, Cardiff UniversityCardiffUK
  2. 2.Department of Dental Health and Biological SciencesDental School, Cardiff UniversityCardiffUK
  3. 3.Health Care Research CenterProctor and GambleMasonUSA
  4. 4.Llandough HospitalMedical School, Cardiff UniversityCardiffUK
  5. 5.Nuffield Department of Orthopedic SurgeryUniversity of OxfordOxfordUK
  6. 6.School of Biosciences, Cardiff UniversityCardiffUK

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