Calcified Tissue International

, Volume 78, Issue 4, pp 227–232 | Cite as

Partial Prevention of Long-Term Femoral Bone Loss in Aged Ovariectomized Rats Supplemented with Choline-Stabilized Orthosilicic Acid

  • M. CalommeEmail author
  • P. Geusens
  • N. Demeester
  • G. J. Behets
  • P. D’Haese
  • J. B. Sindambiwe
  • V. Van Hoof
  • D. Vanden Berghe
Laboratory Investigations


Silicon (Si) deficiency in animals results in bone defects. Choline-stabilized orthosilicic acid (ch-OSA) was found to have a high bioavailability compared to other Si supplements. The effect of ch-OSA supplementation was investigated on bone loss in aged ovariectomized (OVX) rats. Female Wistar rats (n = 58, age 9 months) were randomized in three groups. One group was sham-operated (sham, n = 21), and bilateral OVX was performed in the other two groups. OVX rats were supplemented orally with ch-OSA over 30 weeks (OVX1, n = 20; 1 mg Si/kg body weight daily) or used as controls (OVX0, n = 17). The serum Si concentration and the 24-hour urinary Si excretion of supplemented OVX rats was significantly higher compared to sham and OVX controls. Supplementation with ch-OSA significantly but partially reversed the decrease in Ca excretion, which was observed after OVX. The increase in bone turnover in OVX rats tended to be reduced by ch-OSA supplementation. ch-OSA supplementation increased significantly the femoral bone mineral content (BMC) in the distal region and total femoral BMC in OVX rats, whereas lumbar BMC was marginally increased. Femoral BMD was significantly increased at two sites in the distal region in OVX rats supplemented with ch-OSA compared to OVX controls. Total lumbar bone mineral density was marginally increased by ch-OSA supplementation. In conclusion, ch-OSA supplementation partially prevents femoral bone loss in the aged OVX rat model.


Choline-stabilized orthosilicic acid Silicon Bone Bone mineral density Ovariectomy 



ch-OSA was developed by Dirk Vanden Berghe for Bio Minerals n.v. This study was supported by a grant from the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT, project 000290).


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • M. Calomme
    • 1
    Email author
  • P. Geusens
    • 2
    • 3
  • N. Demeester
    • 1
  • G. J. Behets
    • 4
  • P. D’Haese
    • 4
  • J. B. Sindambiwe
    • 1
  • V. Van Hoof
    • 5
  • D. Vanden Berghe
    • 1
  1. 1.Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary SciencesUniversity of AntwerpBelgium
  2. 2.Biomedical Research InstituteUniversity HasseltAgoralaanBelgium
  3. 3.Department of RheumatologyUniversity HospitalMaastrichtThe Netherlands
  4. 4.Experimental Clinical Pathology and Toxicology, Faculty of MedicineUniversity of AntwerpBelgium
  5. 5.Department of Clinical ChemistryUniversity Hospital AntwerpAntwerpBelgium

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