Partial Prevention of Long-Term Femoral Bone Loss in Aged Ovariectomized Rats Supplemented with Choline-Stabilized Orthosilicic Acid
- 342 Downloads
Silicon (Si) deficiency in animals results in bone defects. Choline-stabilized orthosilicic acid (ch-OSA) was found to have a high bioavailability compared to other Si supplements. The effect of ch-OSA supplementation was investigated on bone loss in aged ovariectomized (OVX) rats. Female Wistar rats (n = 58, age 9 months) were randomized in three groups. One group was sham-operated (sham, n = 21), and bilateral OVX was performed in the other two groups. OVX rats were supplemented orally with ch-OSA over 30 weeks (OVX1, n = 20; 1 mg Si/kg body weight daily) or used as controls (OVX0, n = 17). The serum Si concentration and the 24-hour urinary Si excretion of supplemented OVX rats was significantly higher compared to sham and OVX controls. Supplementation with ch-OSA significantly but partially reversed the decrease in Ca excretion, which was observed after OVX. The increase in bone turnover in OVX rats tended to be reduced by ch-OSA supplementation. ch-OSA supplementation increased significantly the femoral bone mineral content (BMC) in the distal region and total femoral BMC in OVX rats, whereas lumbar BMC was marginally increased. Femoral BMD was significantly increased at two sites in the distal region in OVX rats supplemented with ch-OSA compared to OVX controls. Total lumbar bone mineral density was marginally increased by ch-OSA supplementation. In conclusion, ch-OSA supplementation partially prevents femoral bone loss in the aged OVX rat model.
KeywordsCholine-stabilized orthosilicic acid Silicon Bone Bone mineral density Ovariectomy
ch-OSA was developed by Dirk Vanden Berghe for Bio Minerals n.v. This study was supported by a grant from the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT, project 000290).
- 5.Neer RM, Arnaud CD, Zanchetta JR, Prince R, Gaich GA, Reginster JY, Hodsman AB, Eriksen EF, Ish-Shalom S, Genant HK, Wang O, Mitlak BH (2001) Effect of parathyroid hormone (1-34) on fractures and bone mineral density in postmenopausal women with osteoporosis. N Engl J Med 344:1434–1441CrossRefPubMedGoogle Scholar
- 12.Seaborn CD, Nielsen FH (1994) Dietary silicon affects acid and alkaline phosphatase and 45calcium uptake in bone of rats. J Trace Elem Exp Med 7:11–18Google Scholar
- 19.Calomme M, Cos P, D’Haese P, Vingerhoets R, Lamberts L, De Broe M, Van Hoorebeke C, Vanden Berghe D (2000) Silicon absorption from stabilized orthosilicic acid and other supplements in healthy subjects. In: Roussel AM, Anderson RA, Favier AE (eds) Trace Elements in Man and Animals, vol 10. Kluwer Academic/Plenum Publishers, pp 1111–1114Google Scholar
- 21.Calomme MR, Wijnen P, Sindambiwe JB, Cos P, Mertens J, Geusens P, Vanden Berghe DA (2002) Effect of choline stabilized orthosilicic acid on bone density in chicks. Calcif Tissue Int 70:292Google Scholar
- 27.Van Dyck K, Van Cauwenbergh R, Robberecht H, Deelstra H (1999) Bioavailability of silicon from food and food supplements. Fresenius J Anal Chem 363:541–544Google Scholar
- 29.Spector TD, Calomme MR, Anderson S, Swaminathan R, Jugdaohsingh R, Vanden Berghe D, Powell JJ (2005) Effect on bone turnover and BMD of low dose oral silicon as an adjunct to calcium/vitamin D3 in a randomized, placebo-controlled trial. J Bone Miner Res 20(suppl 1):S172Google Scholar