Calcified Tissue International

, Volume 77, Issue 5, pp 311–318 | Cite as

Prevention of Bone Loss by Phloridzin, an Apple Polyphenol, in Ovariectomized Rats under Inflammation Conditions

  • C. Puel
  • A. Quintin
  • J. Mathey
  • C. Obled
  • M. J. Davicco
  • P. Lebecque
  • S. Kati-Coulibaly
  • M. N. Horcajada
  • V. CoxamEmail author
Laboratory Investigations


Aging and sex hormones related changes lead to inflammatory and oxidant conditions, which are involved in the pathogenesis of osteoporosis. Recent studies have suggested that polyphenols may exert a protective effect in such conditions. We assessed the effect of phloridzin (Phlo), a flavonoid exclusively found in apple, on bone metabolism in ovariectomized (OVX) or sham-operated (SH) rats with and without inflammation. Six-month-old Wistar rats were allocated to two equal groups that received either a control diet or a diet supplemented with 0.25% Phlo for 80 days. Three weeks before necropsy, inflammation was induced by subcutaneous injection of talc in 10 animals of each group. At necropsy, ovariectomy decreased both total (T-BMD) and metaphyseal (M-BMD) femoral bone mineral density (P < 0.01). Inflammation conditions, checked by an increase in the spleen weight and α1-acid glycoprotein concentration in OVX rats, exacerbated the decrease in T-BMD (g/cm2) (as well as M-BMD) observed in castrated animals (P < 0.05). Daily Phlo intake prevented ovariectomy-induced bone loss in conditions of inflammation as shown by T-BMD and M-BMD (P < 0.05). At the diaphyseal site, BMD was improved by Phlo in OVX rats with or without inflammation (P < 0.05). These results could be explained by changes in bone remodeling as the increased urinary deoxypyridinoline excretion in OVX and OVXinf animals was prevented by the polyphenol-rich diet (P < 0.001), while plasma osteocalcin concentration was similar in all experimental groups. In conclusion, Phlo consumption may provide protection against ovariectomy-induced osteopenia under inflammation conditions by improving inflammation markers and bone resorption.

Key words:

Phloridzin Bone-sparing effect Ovariectomized rat Inflammation 


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • C. Puel
    • 1
  • A. Quintin
    • 1
  • J. Mathey
    • 1
  • C. Obled
    • 2
  • M. J. Davicco
    • 1
  • P. Lebecque
    • 1
  • S. Kati-Coulibaly
    • 1
    • 3
  • M. N. Horcajada
    • 1
  • V. Coxam
    • 1
    Email author
  1. 1.Unité des Maladies Métaboliques et MicronutrimentsINRA TheixSaint Genès-ChampanelleFrance
  2. 2.Unité du Métabolisme ProtéiqueINRA TheixSaint Genès-ChampanelleFrance
  3. 3.Laboratoire de Nutrition et PharmacologieUFR Biosciences Université de CocodyCôte d’Ivoire

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