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Calcified Tissue International

, Volume 75, Issue 4, pp 321–328 | Cite as

Effect of the Proportion of Organic Material in Bone on Thermal Decomposition of Bone Mineral: An Investigation of a Variety of Bones from Different Species Using Thermogravimetric Analysis coupled to Mass Spectrometry, High-Temperature X-ray Diffraction, and Fourier Transform Infrared Spectroscopy

  • L. D. Mkukuma
  • J. M. S. Skakle
  • I. R. Gibson
  • C. T. Imrie
  • R. M. Aspden
  • D. W. L. HukinsEmail author
Article

Abstract

Thermogravimetric analysis linked to mass spectrometry (TGA-MS) shows changes in mass and identifies gases evolved when a material is heated. Heating to 600°C enabled samples of bone to be classified as having a high (cod clythrum, deer antler, and whale periotic fin bone) or a low (porpoise ear bone, whale tympanic bulla, and whale ear bone) proportion of organic material. At higher temperatures, the mineral phase of the bone decomposed. High temperature X-ray diffraction (HTXRD) showed that the main solids produced by decomposition of mineral (in air or argon at 800°C to 1000°C) were β-tricalcium phosphate (TCP) and hydroxyapatite (HAP), in deer antler, and CaO and HAP, in whale tympanic bulla. In carbon dioxide, the decomposition was retarded, indicating that the changes observed in air and argon were a result of the loss of carbonate ions from the mineral. Fourier transform infrared (FTIR) spectroscopy of bones heated to different temperatures, showed that loss of carbon dioxide (as a result of decomposition of carbonate ions) was accompanied by the appearance of hydroxide ions. These results can be explained if the structure of bone mineral is represented by
$$ {\text{Ca}}_{{\text{10}} - {\text{x}}} {\text{V}}^{{\text{(Ca)}}} _{\text{x}} [({\text{PO}}_{\text{4}} )_{{\text{6}} - {\text{x}} - {\text{y}}} ({\text{HPO}}_{\text{4}} )_{\text{x}} ({\text{CO}}_{\text{3}} )_{\text{y}} ][({\text{OH}})_{{\text{2}} - {\text{x}} - {\text{y}}} ({\text{CO}}_{\text{3}} )_{\text{y}} {\text{V}}^{{\text{(OH)}}} _{\text{x}} ] $$
where V(Ca) and V(OH) correspond to vacancies on the calcium and hydroxide sites, respectively, and 2−x−y = 0.4. This general formula is consistent in describing both mature bone mineral (i.e., whale bone), with a high Ca/P molar ratio, lower HPO 4 2− content, and higher CO 3 2− content, and immature bone mineral (i.e., deer antler), with a low Ca/P ratio, higher HPO 4 2− , and lower CO 3 2− content.

Keywords

Infrared spectroscopy Thermogravimetric analysis Mass spectrometry Mineral - organic content X-ray diffraction 

Notes

Acknowledgments

We thank Dr. S. Lees and Dr. S. D. Mehta for the gift of the bones used in this study. Financial support was provided by the Engineering and Physical Sciences Research Council (UK). R.M.A. was supported by a Medical Research Council Senior Fellowship.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • L. D. Mkukuma
    • 1
    • 5
  • J. M. S. Skakle
    • 2
  • I. R. Gibson
    • 3
  • C. T. Imrie
    • 2
  • R. M. Aspden
    • 1
  • D. W. L. Hukins
    • 4
    • 6
    Email author
  1. 1.Department of Orthopaedic SurgeryUniversity of AberdeenForesterhillUK
  2. 2.Department of ChemistryUniversity of AberdeenAberdeenUK
  3. 3.Department of Biomedical SciencesUniversity of AberdeenForesterhillUK
  4. 4.Department of Bio-Medical Physics & Bio-EngineeringUniversity of AberdeenForesterhillUK
  5. 5.Queen Elizabeth Central HospitalBlantyre
  6. 6.School of Engineering, Mechanical EngineeringUniversity of BirminghamEdgbastonUK

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