Calcified Tissue International

, Volume 74, Issue 2, pp 150–156 | Cite as

Effect of Vitamins D2 and D3 Supplement Use on Serum 25OHD Concentration in Elderly Women in Summer and Winter

Clinical Investigations

Abstract

Vitamin D2 and D3 are generally considered equipotent in humans. A few studies have reported that serum 25OHD levels are higher in vitamin D3- compared with vitamin D2-supplemented subjects. As both vitamin D2 and D3 supplements are commonly used by elderly in United States, in the present study we determined the effect of self-reported vitamin D2 and vitamin D3 supplement use on serum total 25OHD levels according to season in elderly women aged 65–77 years. Serum total 25OHD levels were determined in winter and summer in unsupplemented women (N = 307) and in women who reported taking vitamin D2 (N = 56) and vitamin D3 (N = 55) supplements by competitive protein binding assay. In vitamin D2-supplemented women, the contribution of vitamin D2 and D3 to the mean serum total 25OHD level was assessed by HPLC. In summer, there were no significant differences in the mean total serum 25OHD levels (ng/ml) among the vitamin D2 (32 ± 2.1), vitamin D3 (36.7 ± 1.95), and unsupplemented (32.2 ± 0.95) groups. In winter, the mean serum total 25OHD levels were higher in women on vitamin D2 (33.6 ± 2.34, P < 0.05) and vitamin D3 (29.7 ± 1.76, NS) supplements compared with unsupplemented women (27.3 ± 0.72). In vitamin D2-supplemented women, about 25% of the mean serum total 25OHD was 25OHD2, in both summer and winter. Twelve percent of unsupplemented women and 3.6% of vitamin D-supplemented women had a mean serum total 25OHD level below 15 ng/ml in winter. In elderly subjects, both vitamin D2 and Vitamin D3 supplements may contribute equally to circulating 25OHD levels, with the role of vitamin D supplement use being more predominant during winter.

Keywords

25-Hydroxyvitamin D 25OHD HPLC Vitamin D supplement Competitive protein binding assay 25OHD2 

Notes

Acknowledgements

This work was supported by National Institute of Health Research grants UO1-AG10373 and RO1-AGI0358. We thank Karen A. Rafferty for her help in food diary data collection and analysis.

References

  1. 1.
    Baker, MR, McDonnell, H, Peacock, M, Nordin, BE 1979Plasma 25-hydroxy vitamin D concentrations in patients with fractures of the femoral neck.Br Med J1589Google Scholar
  2. 2.
    Boonen, S, Aerssens, J, Dequeker, J 1996Age-related endocrine deficiencies and fractures of the proximal femur. II implications of vitamin D deficiency in the elderly.J Endocrinol1491317PubMedGoogle Scholar
  3. 3.
    Gloth III, FM, Tobin, JD 1995Vitamin D deficiency in older people.J Am Geriatr soc43822828Google Scholar
  4. 4.
    Chapuy, MC, Arlot, ME, Duboeuf, F, Brun, J, Crouzet, B, Arnaud, S, Delmas, PD, Meunier, PJ 1992Vitamin D3 and calcium to prevent hip fractures in the elderly women.N Engl J Med32716371642PubMedGoogle Scholar
  5. 5.
    Holick, MF, Matsuoka, LY, Wortsman, J 1989Age, vitamin D, and solar ultraviolet.Lancet211041105CrossRefGoogle Scholar
  6. 6.
    MacLaughlin, J, Holick, MF 1985Aging decreases the capacity of human skin to produce vitamin D3.J Clin Invest7615361538PubMedGoogle Scholar
  7. 7.
    Need, AG, Morris, HA, Horowitz, M, Nordin, C 1993Effects of skin thickness, age, body fat, and sunlight on serum25-hydroxyvitamin D.Am J Clin Nutr58882885PubMedGoogle Scholar
  8. 8.
    Chapuy, MC, Arlot, ME, Delmas, PD, Meunier, PJ 1994Effect of calcium and cholecalciferol treatment for three years on hip fractures in elderly women.BMJ30810811082PubMedGoogle Scholar
  9. 9.
    Reid, IR 1998The roles of calcium and vitamin D in the prevention of osteoporosis.Endocrinol Metab Clin North Am27389398Google Scholar
  10. 10.
    Reid, IR 1996Therapy of osteoporosis: calcium, vitamin D, and exercise.Am J Med Sci312278286CrossRefPubMedGoogle Scholar
  11. 11.
    Dawson–Hughes, B, Harris, SS, Krall, EA, Dallal, GE, Falconer, G, Green, CL 1995Rates of bone loss in postmenopausal women randomly assigned to one of two dosages of vitamin D.Am J Clin Nutr6111401145PubMedGoogle Scholar
  12. 12.
    Balluz, LS, Kieszak, SM, Philen, RM, Mulinare, J 2000Vitamin and mineral supplement use in the United States. Results from the third National Health and Nutrition Examination Survey.Arch Fam Med9258262PubMedGoogle Scholar
  13. 13.
    Margiloff, L, Harris, SS, Lee, S, Lechan, R, Dawson-Hughes, B 2001Vitamin D status of an outpatient clinic population.Calcif Tissue Int69263267CrossRefPubMedGoogle Scholar
  14. 14.
    Horst, RL, Napoli, JL, Littledike, ET 1982Discrimination in the metabolism of orally dosed ergocalciferol and cholecalciferol by the pig, rat and chick.Biochem J204185189PubMedGoogle Scholar
  15. 15.
    Hunt, RD, Garcia, FG, Walsh, RJ 1972A comparison of the toxicity of ergocalciferol and cholecalciferol in rhesus monkeys (Macaca mulatta).J Nutr102975986PubMedGoogle Scholar
  16. 16.
    Marx, SJ, Jones, G, Weinstein, RS, Chrousos, GP, Renquist, DM 1989Differences in mineral metabolism among nonhuman primates receiving diets with only vitamin D3 or only vitamin D2.J Clin Endocrinol Metab6912821290Google Scholar
  17. 17.
    Holick, MF, DeLuca, HF 1978

    Metabolism of vitamin D.

    Lawson, DEM eds. Vitamin D.AcademicNew York5191
    Google Scholar
  18. 18.
    Norman, AW 1979Vitamin D: The calcium homeostatic steroid hormone.Academic PressNew YorkGoogle Scholar
  19. 19.
    Tjellesen, L, Christiansen, C, Rodbro, P, Hummer, L 1985Different metabolism of vitamin D2 and vitamin D3 in epileptic patients on carbamazepine.Acta Neurol Scand71385389PubMedGoogle Scholar
  20. 20.
    Tjellesen, L, Hummer, L, Christiansen, C, Rodbro, P 1986Different metabolism of vitamin D2/D3 in epileptic patients treated with phenobarbitone/phenytoin.Bone7337342PubMedGoogle Scholar
  21. 21.
    Trang, HM, Cole, DE, Rubin, LA, Pierratos, A, Siu, S, Vieth, R 1998Evidence that vitamin D3 increases serum 25-hydroxyvitamin D more efficiently than does vitamin D2.Am J Clin Nutr68854858PubMedGoogle Scholar
  22. 22.
    Chapuy, MC, Chapuy, P, Meunier, PJ 1987Calcium and vitamin D supplements: effects on calcium metabolism in elderly people.Am J Clin Nutr46324328PubMedGoogle Scholar
  23. 23.
    Dawson–Hughes, B, Dallal, GE, Krall, EA, Harris, S, Sokoll, LJ, Falconer, G 1991Effect of vitamin D supplementation on wintertime and overall bone loss in healthy postmenopausal women,Ann Intern Med115505512PubMedGoogle Scholar
  24. 24.
    Krall, EA, Sahyoun, N, Tannenbaum, S, Dallal, GE, Dawson–Hughes, B 1989Effect of vitamin D intake on seasonal variations in parathyroid hormone secretion in postmenopausal women.N Engl J Med32117771783PubMedGoogle Scholar
  25. 25.
    Omdahl, JL, Garry, PJ, Hunsaker, LA, Hunt, WC, Goodwin, JS 1982Nutritional status in a healthy elderly population: vitamin D.Am J Clin Nutr3612251233PubMedGoogle Scholar
  26. 26.
    Perry III, HM, Horowitz, M, Morley, JE, Patrick, P, Vellas, B, Baumgartner, R, Garry, PJ 1999Longitudinal changes in serum 25-hydroxyvitamin D in older people.Metabolism4810281032Google Scholar
  27. 27.
    Webb, AR, Pilbeam, C, Hanafm, N, Holick, MF 1990An evaluation of the relative contributions of exposure to sunlight and of diet to the circulating concentrations of 25-hydroxyvitamin D in an elderly nursing home population in Boston.Am J Clin Nutr5110751081PubMedGoogle Scholar
  28. 28.
    Gallagher, JC, Fowler, SE, Detter, JR, Sherman, SS 2001Combination treatment with estrogen and calcitriol in the prevention of age-related bone loss.J Clin Endocrinol Metab8636183628Google Scholar
  29. 29.
    Haddad, JG, Chyu, KJ 1971Competitive protein-binding radioassay for 25-hydroxycholecalciferol.J Clin Endocrinol Metab33992995Google Scholar
  30. 30.
    Reinhardt, TA, Horst, RL 1988

    Simplified assays for the determination of 25-OHD, 24,25-(OH)2D and 1,25-(OH)2D.

    Norman, AWSchaefer, KGrigoleit, HG eds. Vitamin D, Molecular, Cellular, and Clinical Endocrinology.Walter de Gruyter & Co.Berlin720726
    Google Scholar
  31. 31.
    Wei, S, Tanaka, H, Kubo, T, Ichikawa, M, Seino, Y 1994A multiple assay for vitamin D metabolites without high-performance liquid chromatography.Anal Biochem222359365CrossRefPubMedGoogle Scholar
  32. 32.
    Hummer, L, Tjellesen, L, Rickers, H, Christiansen, C 1984Measurement of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in clinical settings.Scand J Clin Lab Invest44595601PubMedGoogle Scholar
  33. 33.
    Norris, RL, Thomas, MJ, Craswell, PW 1986Assessment of a two-step high-performance liquid chromatographic assay using dual-wavelength ultraviolet monitoring for 25-hydroxyergocalciferol and 25-hydroxycholecalciferol in human serum or plasma.J Chromatogr3815361CrossRefPubMedGoogle Scholar
  34. 34.
    Eisman, JA, Hamstra, AJ, Kream, BE, DeLuca, HF 1976A sensitive, precise, and convenient method for determination of 1,25-dihydroxyvitamin D in human plasma.Arch Biochem Biophys176235243PubMedGoogle Scholar
  35. 35.
    Jones, G 1978Assay of vitamins D2 and D3, and 25-hydroxyvitamins D2 and D3 in human plasma by high-performance liquid chromatography.Clin Chem24287298PubMedGoogle Scholar
  36. 36.
    Bhattacharyya, MH, DeLuca, HF 1973The regulation of rat liver calciferol-25-hydroxylase.J Biol Chem24829692973PubMedGoogle Scholar
  37. 37.
    Clemens, TL, Zhou, XY, Myles, M, Endres, D, Lindsay, R 1986Serum vitamin D2 and vitamin D3 metabolite concentrations and absorption of vitamin D2 in elderly subjects.J Clin Endocrinol Metab63656660Google Scholar
  38. 38.
    Hartwell, D, Hassager, C, Christiansen, C 1987Effect of vitamin D2 and vitamin D3 on the serum concentrations of 1,25(OH)2D2, and 1,25(OH)2D3 in normal subjects.Acta Endocrinol (Copenh)115378384Google Scholar
  39. 39.
    Himmelstein, S, Clemens, TL, Rubin, A, Lindsay, R 1990Vitamin D supplementation in elderly nursing home residents increases 25(OH)D but not 1,25(OH)2D.Am J Clin Nutr52701706PubMedGoogle Scholar
  40. 40.
    Dawson–Hughes, B, Harris, SS, Dallal, GE 1997Plasma calcidiol, season, and serum parathyroid hormone concentrations in healthy elderly men and women.Am J Clin Nutr656771PubMedGoogle Scholar
  41. 41.
    Rapuri, PB, Kinyamu, HK, Gallagher, JC, Haynatzka, V 2002Seasonal changes in calciotropic hormones, bone markers, and bone mineral density in elderly women.J Clin Endocrinol Metab8720242032Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Bone Metabolism UnitCreighton University, School of Medicine, Omaha, NE 68131USA
  2. 2.Department of MedicineCreighton University, Omaha, NE 68131USA

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