Calcified Tissue International

, Volume 73, Issue 5, pp 470–475 | Cite as

The Influence of Fluoride Exposure on Dentin Mineralization Using an in Vitro Organ Culture Model

  • R. Moseley
  • R. J. WaddingtonEmail author
  • A. J. Sloan
  • A. J. Smith
  • R. C. Hall
  • G. Embery
Laboratory Investigations


This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing the apparent changes in mineral composition.


Dentin Mineralization Fluoride Odontoblasts 



We gratefully acknowledge the funding of this research by The Wellcome Trust (Grant N° 054420) and for a Research Fellowship to support Dr. R. Moseley. We also wish to express our gratitude to Mrs. G. Smith and Mrs. W. G. Rowe for their assistance during the course of this study.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • R. Moseley
    • 1
    • 4
  • R. J. Waddington
    • 2
    Email author
  • A. J. Sloan
    • 2
  • A. J. Smith
    • 2
  • R. C. Hall
    • 3
  • G. Embery
    • 3
  1. 1.Matrix Biology & Tissue Repair Research Unit, Department of Dental Health & Biological SciencesDental School, University of Wales College of Medicine, Heath Park, CardiffUK
  2. 2.Oral BiologySchool of Dentistry, St. Chads Queensway, BirminghamUK
  3. 3.Department of Clinical Dental Sciences, Edwards Building, School of DentistryUniversity of Liverpool, Daulby Street, LiverpoolUK
  4. 4.Department of Oral Surgery, Medicine & Pathology, Dental School, University of Wales College of Medicine, CardiffUK

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