Calcified Tissue International

, Volume 73, Issue 1, pp 27–32 | Cite as

Leptin, Body Composition and Bone Mineral Density in Premenopausal Women

  • M. Blum
  • S. S. Harris
  • A. Must
  • E. N. Naumova
  • S. M. Phillips
  • W. M. Rand
  • B. Dawson-Hughes
Clinical Investigations

Abstract

Body weight is known to be associated with bone mass, however, it is unclear whether body composition, as reflected by the percent of total weight that is fat tissue (%fat), is associated with bone mass independently of weight. Fat tissue is metabolically active, and hormonal factors may mediate an association of %fat with bone mass. Leptin, a hormone produced in fat tissue, has recently been shown to be inversely related to bone mass in mice, but whether it is related to human bone mass is uncertain. We sought to investigate the associations of %fat and of serum leptin concentration with bone mineral density (BMD) in a cohort of 153 premenopausal women. BMD measurements of the total hip, lumbar spine and total body as well as body composition were measured by dual energy X-ray absorptiometry (DXA). Serum leptin levels were established using a commercial competitive binding assay. Individually, body weight, %fat and leptin were each positively associated with BMD at all three sites. However, when we examined BMD either as a function of both body weight and %fat together, or as a function of both body weight and leptin together, we found that for a given body weight, BMD appeared to be inversely associated with %fat and similarly appeared to be inversely associated with leptin. When BMD was examined as a function of %fat and leptin together, we found that for a given %fat, leptin appeared to be inversely associated with BMD. In summary, the results of this study suggest that for a given body weight, a higher proportion of fat and a higher serum leptin concentration have negative associations with bone mass in premenopausal women.

Keywords

Bone mass Body composition Percent fat Leptin 

References

  1. 1.
    Pocock, N, Eisman, J, Gwinn, T, Sambrook, P, Kelly, P, Freund, J, Yeates, M 1989Muscle strength, physical fitness, and weight but not age predict femoral neck bone mass.J Bone Miner Res4441448PubMedGoogle Scholar
  2. 2.
    Glastre, C, Braillon, P, David, L, Cochat, P, Meunier, PS, Delmas, PD 1990Measurement of bone mineral content of the lumbar spine by dual energy X-ray absorptiometry in normal children: correlations with growth parameters.J Clin Endocrinol Metab7013301333PubMedGoogle Scholar
  3. 3.
    Fehily, AM, Coles, RJ, Evans, WD, Elwood, PC 1992Factors affecting bone density in young adults.Am J Clin Nutr56579586PubMedGoogle Scholar
  4. 4.
    Ooms, ME, Lips, P, Van Lingen, A, Valkenburg, HA 1993Determinants of bone mineral density and risk factors for osteoporosis in healthy elderly women.J Bone Miner Res8669675PubMedGoogle Scholar
  5. 5.
    Aloia, JF, Vaswani, A, Ma, R, Flaster, E 1995To what extent is bone mass determined by fat-free or fat mass?Am J Clin Nutr6111101114PubMedGoogle Scholar
  6. 6.
    Lanyon, LE 1992Control of bone architecture by functional load bearing.J Bone Miner Res7S369S375PubMedGoogle Scholar
  7. 7.
    Lindsay, R, Cosman, F, Herrington, BS, Himmelstein, S 1992Bone mass and body composition in normal women.J Bone Miner Res75563PubMedGoogle Scholar
  8. 8.
    Sowers, MF, Kshirsagar, A, Crutchfield, MM, Updike, S 1992Joint influence of fat and lean body composition compartments on femoral bone mineral density in premenopausal women.Am J Epidemiol136257265PubMedGoogle Scholar
  9. 9.
    Compston, JE, Bhambhani, M, Laskey, MA, Murphy, S, Khaw, KT 1992Body composition and bone mass in post-menopausal women.Clin Endocrinol37426431Google Scholar
  10. 10.
    Takada, H, Washino, K, Iwata, H 1997Risk factors for low bone mineral density among females: the effect of lean body mass.Prev Med26633638CrossRefPubMedGoogle Scholar
  11. 11.
    Reid, IR, Ames, R, Evans, MC, Sharpe, S, Gamble, G, France, JT, Lim, TM, Cundy, TF 1992Determinants of total body and regional bone mineral density in normal postmenopausal women — a key role for fat mass.J Clin Endocrinol Metab754551PubMedGoogle Scholar
  12. 12.
    Reid, IR, Evans, MC, Ames, RW 1994Volumetric bone density of the lumbar spine is related to fat mass but not lean mass in normal postmenopausal women.Osteoporosis Int4362367Google Scholar
  13. 13.
    Bell, NH, Epstein, S, Greene, A, Shary, J, Oexmann, MJ, Shaw, S 1985Evidence for alteration of the vitamin D-endocrine system in obese subjects.J Clin Invest76370373PubMedGoogle Scholar
  14. 14.
    Liel, Y, Ulmer, E, Shary, J, Hollis, BW, Bell, NH 1988Low circulating vitamin D in obesity.Calcif Tissue Int43199201PubMedGoogle Scholar
  15. 15.
    Tremollieres, F, Pouilles, JM, Ribot, C 1993Vertebral postmenopausal bone loss is reduced in overweight women: a longitudinal study in 155 early postmenopausal women.J Clin Endocrionol Metab77683686Google Scholar
  16. 16.
    Haffner, SM, Bauer, RL 1992Excess androgenicity only partially explains the relationship between obesity and bone density in premenopausal women.Int J Obes Relat Metab Disord16869874PubMedGoogle Scholar
  17. 17.
    Reid, IR, Evans, ML, Cooper, GJ, Ames, RW, Stapleton, J 1993Circulating insulin levels are related to bone density in normal postmenopausal women.Am J Physiol265E655659PubMedGoogle Scholar
  18. 18.
    Ducy, P, Amling, M, Takeda, S, Priemel, M, Schilling, AF, Beil, FT, Shen, J, Vinson, C, Rueger, JM, Karsenty, G 2000Leptin inhibits bone formation through a hypothalamic relay: a central control of bone mass.Cell100197207PubMedGoogle Scholar
  19. 19.
    Goulding, A, Taylor, RW 1998Plasma leptin values in relation to bone mass and density and to dynamic biochemical markers of bone resorption and formation in postmenopausal women.Calcif Tissue Int63456458PubMedGoogle Scholar
  20. 20.
    Kiel, D, Harris, T, Visser, M, Hannan, M, Wilson, P 1999Leptin and bone mineral density (BMD) in older men and women.J Bone Miner Res14SU475Google Scholar
  21. 21.
    Martini, G, Valenti, R, Giovani, S, Franci, B, Campagna, S, Nuti, R 2001Influence of insulin-like growth factor-1 and leptin on bone mass in healthy postmenopausal women.Bone28113117Google Scholar
  22. 22.
    Rauch, F, Blum, WF, Klein, K, Allolio, B, Schonau, E 1998Does leptin have an effect on bone in adult women?Calcif Tissue Int63453455PubMedGoogle Scholar
  23. 23.
    Matkovic, V, Ilich, JZ, Skugor, M, Badenhop, NE, Goel, P, Clairmont, A, Klisovic, D, Nahhas, RHW, Landoll, JO 1997Leptin is inversely related to age at menarche in human females.J Clin Endocrinol Metab8232393245PubMedGoogle Scholar
  24. 24.
    Odabasi, E, Ozata, M, Turan, M, Bingol, N, Yonem, A, Cakir, B, Kutlu, M, Ozdemir, IC 2000Plasma leptin concentrations in postmenopausal women with osteoporosis.Eur J Endocrinol142170173Google Scholar
  25. 25.
    Iwamoto, I, Oouchi, T, Kosha, A, Murakami, M, Fujino, T, Nagata, Y 2000Relationships between serum leptin level and regional bone mineral density, bone metabolic markers in healthy women.Acta Obstet Gynecol Scandinavica7910601064CrossRefGoogle Scholar
  26. 26.
    Zacharias, L, Rand, WM, Wurtman, RJ 1976A prospective study of sexual development and growth in American girls: the statistics of menarche.Obstet Gynecol Surv31325337PubMedGoogle Scholar
  27. 27.
    Zacharias, L, Rand, WM 1983Adolescent growth in height and its relation to menarche in contemporary American girls.Ann Hum Biol10209222PubMedGoogle Scholar
  28. 28.
    Zacharias, L, Rand, WM 1986Adolescent growth in weight and its relationship to menarche in contemporary American girls.Ann Hum Biol13369386PubMedGoogle Scholar
  29. 29.
    Block, G, Woods, M, Potosky, A, Clifford, C 1990Validation of a self-administered diet history questionnaire using multiple diet records.J Clin Epidemiol4313271335PubMedGoogle Scholar
  30. 30.
    Johnson, J, Dawson-Hughes, B 1991Precision and stability of dual-energy x-ray absorptiometry measurements.Calcif Tissue Int49174178PubMedGoogle Scholar
  31. 31.
    Khosla, S, Atkinson, EJ, Riggs, BL, Melton, LJ 1996Relationship between body composition and bone mass in women.J Bone Miner Res11857863PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • M. Blum
    • 1
  • S. S. Harris
    • 1
  • A. Must
    • 2
  • E. N. Naumova
    • 2
  • S. M. Phillips
    • 2
  • W. M. Rand
    • 2
  • B. Dawson-Hughes
    • 1
  1. 1.Calcium and Metabolic Bone LaboratoryJean Mayer United States Department of Agriculture, Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts 02111USA
  2. 2.Department of Family Medicine and Community HealthTufts University, Boston, Massachusetts 02111USA

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