Calcified Tissue International

, Volume 71, Issue 3, pp 243–248

Hydrogen Peroxide Induces Apoptosis of Osteocytes: Involvement of Calcium Ion and Caspase Activity

  • A. Kikuyama
  • K. Fukuda
  • S. Mori
  • M. Okada
  • H. Yamaguchi
  • C. Hamanishi
Article

DOI: 10.1007/s00223-001-1110-2

Cite this article as:
Kikuyama, A., Fukuda, K., Mori, S. et al. Calcif Tissue Int (2002) 71: 243. doi:10.1007/s00223-001-1110-2

Abstract

We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activation of caspase-3 and increase in cytosolic Ca2+. Treatment with BAPTA/AM (cheletor of cytosolic Ca2+) and Ac-DEVD-cho (caspase inhibitor) attenuated hydrogen peroxide-induced apoptosis. These data demonstrated the signal transduction pathways that participate in this hydrogen peroxide-induced cell damage.

Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • A. Kikuyama
    • 1
  • K. Fukuda
    • 1
  • S. Mori
    • 1
  • M. Okada
    • 1
  • H. Yamaguchi
    • 1
  • C. Hamanishi
    • 1
  1. 1.Department of Orthopaedic Surgery, Kinki University School of Medicine, Ohno-higashi 377-2, Osaka-sayama, Osaka 589-8511, JapanJP

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