Hydrogen Peroxide Induces Apoptosis of Osteocytes: Involvement of Calcium Ion and Caspase Activity
- Cite this article as:
- Kikuyama, A., Fukuda, K., Mori, S. et al. Calcif Tissue Int (2002) 71: 243. doi:10.1007/s00223-001-1110-2
We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activation of caspase-3 and increase in cytosolic Ca2+. Treatment with BAPTA/AM (cheletor of cytosolic Ca2+) and Ac-DEVD-cho (caspase inhibitor) attenuated hydrogen peroxide-induced apoptosis. These data demonstrated the signal transduction pathways that participate in this hydrogen peroxide-induced cell damage.