Experimental Brain Research

, Volume 130, Issue 1, pp 100–104 | Cite as

Constitutive NF-κB activity is modulated via neuron-astroglia interaction

  • B. Kaltschmidt
  • C. Kaltschmidt
Research Note


NF-κB is found in many neuronal cell types in different states of activity. This study aimed to define which conditions induce constitutive NF-κB activity in cultured hippocampal neurons using activity-specific antibody staining. In co-culture with astroglia, hippocampal neurons were devoid of activated NF-κB. In these co-cultures, NF-κB could not be activated via kainate or glutamate. In contrast, separating neurons from the glial compartment resulted in a time-dependent increase of activated neuronal NF-κB. In this line, activation of NF-κB by kainate or glutamate is very effective in freshly separated cultures, but inhibited when the cultures are reassembled after stimulation. These findings suggests that a neuronal-glial interaction may regulate gene expression via NF-κB.

Key words NF-κB p65 Hippocampal neurons Glia Astrocytes Rat 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • B. Kaltschmidt
    • 2
  • C. Kaltschmidt
    • 1
  1. 1.Molecular Neurobiology Laboratory, Albert-Ludwigs-University, Stefan-Meier-Str. 19, D-79104 Freiburg, Germany e-mail: Tel.: +49-203-761-5115, Fax: +49-203-761-5117DE
  2. 2.Institute of Anatomy, Albert-Ludwigs-University, D-79104 Freiburg, GermanyDE

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