Experimental Brain Research

, Volume 235, Issue 9, pp 2893–2899 | Cite as

Effect of post-weaning isolation on anxiety- and depressive-like behaviors of C57BL/6J mice

  • Qian Huang
  • Ying Zhou
  • Lin-Yun LiuEmail author
Research Article


Effects of post-weaning isolation on depressive- and anxiety-like behaviors in rodents have been well studied in the past. However, few studies included both sexes in a single experiment to study the sex difference in this animal model. The present study investigated the effect of post-weaning isolation on anxiety- and depressive-like behaviors in both male and female C57BL/6 J mice. Mice were individually or grouped housed from postnatal day 21 for 5 weeks until behavioral tests began. The results showed that social isolation resulted in increased anxiety in the open field. Isolated-reared female, but not male mice showed an increased transition between two compartments in the light–dark box and a decreased immobile time in the forced swim test. We conclude that post-weaning isolation has a sex-specific effect on emotional behaviors.


Social isolation Anxiety Depression Sex difference Mice 



This work was supported by grants from the National Natural Science Foundation of China (31200827) and Shanghai Municipal Commission of Health and Family Planning (20134Y134).

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interests.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain ScienceFudan UniversityShanghaiChina

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