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Experimental Brain Research

, Volume 234, Issue 11, pp 3225–3232 | Cite as

Near-infrared light treatment reduces astrogliosis in MPTP-treated monkeys

  • Nabil El Massri
  • Cécile Moro
  • Napoleon Torres
  • Fannie Darlot
  • Diane Agay
  • Claude Chabrol
  • Daniel M. Johnstone
  • Jonathan Stone
  • Alim-Louis Benabid
  • John Mitrofanis
Research Article

Abstract

We have reported previously that intracranial application of near-infrared light (NIr) reduces clinical signs and offers neuroprotection in a subacute MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) monkey model of Parkinson’s disease. In this study, we explored whether NIr reduces the gliosis in this animal model. Sections of midbrain (containing the substantia nigra pars compacta; SNc) and striatum were processed for glial fibrillary acidic protein (to label astrocytes; GFAP) and ionised calcium-binding adaptor molecule 1 (to label microglia; IBA1) immunohistochemistry. Cell counts were undertaken using stereology, and cell body sizes were measured using ImageJ. Our results showed that NIr treatment reduced dramatically (~75 %) MPTP-induced astrogliosis in both the SNc and striatum. Among microglia, however, NIr had a more limited impact in both nuclei; although there was a reduction in overall cell size, there were no changes in the number of microglia in the MPTP-treated monkeys after NIr treatment. In summary, we showed that NIr treatment influenced the glial response, particularly that of the astrocytes, in our monkey MPTP model of Parkinson’s disease. Our findings raise the possibility of glial cells as a future therapeutic target using NIr.

Keywords

Substantia nigra Striatum Parkinson’s disease Photobiomodulation 670 nm 

Abbreviations

ATP

Adenosine triphosphate

GFAP

Glial fibrillary acidic protein

GP

Globus pallidus

IBA1

Ionised calcium-binding adaptor molecule 1

MPTP

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine

NIr

Near-infrared light

R

Red nucleus

SNc

Substantia nigra pars compacta

SNr

Substantia nigra pars reticulata

Notes

Acknowledgments

We are forever grateful to Michael J Fox Foundation, Credit Agricole Sud Rhones Alpes, Fondation Philanthropique Edmond J Safra, France Parkinson and the French National Research Agency (ANR Carnot Institute), Tenix corp and Salteri family and our industry partners for funding this work. Daniel Johnstone is an Early Career Fellow of the NHMRC, Australia. We thank Sharon Spana, Diane Agay, Guillaume Barboux, Clément Perrin, Cyril Zenga and Mylène D’Orchymont for excellent technical assistance.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Nabil El Massri
    • 1
  • Cécile Moro
    • 2
  • Napoleon Torres
    • 2
  • Fannie Darlot
    • 2
  • Diane Agay
    • 2
  • Claude Chabrol
    • 2
  • Daniel M. Johnstone
    • 3
  • Jonathan Stone
    • 3
  • Alim-Louis Benabid
    • 2
  • John Mitrofanis
    • 1
  1. 1.Department of Anatomy F13University of SydneySydneyAustralia
  2. 2.University Grenoble AlpesGrenobleFrance
  3. 3.Department of Physiology F13University of SydneySydneyAustralia

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