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Experimental Brain Research

, Volume 212, Issue 1, pp 143–152 | Cite as

Transcranial magnetic stimulation of macaque frontal eye fields decreases saccadic reaction time

  • Annelies Gerits
  • Christian C. Ruff
  • Olivier Guipponi
  • Nicole Wenderoth
  • Jon Driver
  • Wim VanduffelEmail author
Research Article

Abstract

Transcranial magnetic stimulation (TMS) is increasingly used to perturb targeted human brain sites non-invasively, to test for causal effects on performance of cognitive tasks. TMS might also be used in non-human primates to complement invasive work and compare with human studies. Here, we targeted the frontal eye fields (FEF) in two macaques with a continuous theta-burst (cTBS) protocol, testing the impact on visually guided saccades. After unilateral cTBS over the FEF in either hemisphere, a small (mean 7 ms) but highly consistent decrease in saccadic reaction times (RTs) was observed. Lower latencies arose for saccades both contra- and ipsilateral to the stimulated FEF after cTBS. These results provide the first demonstration that TMS can be used to affect saccadic behavior in non-human primates. The unexpectedly bilateral impact on RTs may reflect an impact on ‘fixation’ neurons in the FEF and/or transcallosal modulation of both FEFs induced by unilateral cTBS. In either case, this study demonstrates a clear behavioral effect induced by TMS in awake behaving monkeys performing a cognitive task. This opens new opportunities for investigating the causal roles of targeted brain areas in behavior, for measuring physiological consequences of TMS in the primate brain, and ultimately for human–monkey comparisons.

Keywords

Frontal eye field Macaque Saccades Theta burst TMS 

Notes

Acknowledgments

The authors are indebted to A. Coeman, C. Fransen, M. Depaep, W. Depuydt, M. Deforche, P. Kayenbergh, G. Meulemans, C. Van Eupen, S. Verstraeten for help with the experiments. The work was supported by Human Frontiers Science Program (HFSPO RGP7/2007), Medical Foundation Queen Elisabeth (2008-10), Inter University Attraction Pole6/29, Program Financing 2010-15, Fonds voor Wetenschappelijk onderzoek, VLaanderen (FWO) G.0622.08; FWO G.0593.09, National Science Foundation BCS-0745436 and European Union (EU) grant (FP7 HEALTH-F2-2008-200728), BrainSynch. JD is a Royal Society Anniversary Research Professor. AG has an Aspirant fellowship of FWO-Flanders.

Supplementary material

Supplementary material 1 (MPG 8480 kb)

221_2011_2710_MOESM2_ESM.eps (10.6 mb)
Fig. S1 Schematic drawing of a monkey-TMS setup based on a picture. The left panel of the figure shows the D25mm TMS coil with its center positioned above the monkey’s right FEF. The position was determined using the brainsight neuronavigation system. The head of the monkey was fixed to the chair. In the right panel the size of TMS coil in respect to the monkey’s head size can be observed. (EPS 10892 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Annelies Gerits
    • 1
    • 2
    • 3
  • Christian C. Ruff
    • 5
    • 6
    • 7
  • Olivier Guipponi
    • 1
  • Nicole Wenderoth
    • 4
  • Jon Driver
    • 6
    • 7
  • Wim Vanduffel
    • 1
    • 2
    • 3
    Email author
  1. 1.Laboratory for Neuro-and Psychophysiology, K.U. Leuven Medical School, Campus GasthuisbergLeuvenBelgium
  2. 2.Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General HospitalCharlestownUSA
  3. 3.Department of RadiologyHarvard Medical SchoolCharlestownUSA
  4. 4.Laboratory for Movement Control and Neuroplasticity, K.U. LeuvenLeuvenBelgium
  5. 5.Laboratory for Social and Neural Systems Research (SNS-lab)ZurichSwitzerland
  6. 6.UCL Institute of Cognitive NeuroscienceLondonUK
  7. 7.Wellcome Trust Centre for Neuroimaging at UCLLondonUK

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