Experimental Brain Research

, Volume 159, Issue 2, pp 185–196 | Cite as

Retinal projections to the lateral posterior-pulvinar complex in intact and early visual cortex lesioned cats

  • Denis Boire
  • Isabelle Matteau
  • Christian Casanova
  • Maurice Ptito
Research Article


In intact cats, it is generally considered that the lateral posterior-pulvinar complex (LP-pulvinar) does not receive direct retinal terminals, with the exception of the retino-recipient zone known as the geniculate wing. There is, however, some evidence that early lesions of the visual cortex can occasionally induce the formation of novel retinal projections to the LP nucleus. Given the importance of knowing the connectivity pattern of the LP-pulvinar complex in intact and lesioned animals, we used the B fragment of cholera toxin, a sensitive anterograde tracer, to reinvestigate the retinal projections to the LP-pulvinar in normal cats and in cats with early unilateral lesions of the visual cortex (areas 17 and 18). Immunohistochemical localization of the toxin was performed to show the distribution and morphology of retinofugal terminals. A direct bilateral but predominantly contralateral retinal projection reached the caudal portion of LPl and LPm in the form of patches located mainly along its dorsomedial surface and many scattered terminals. The distribution of retinal projections to LP-pulvinar in intact and operated cats did not differ. Contrary to what had been previously reported, we found no evidence for lesion-induced sprouting of retinal axons in these higher-order thalamic nuclei. Retinal input to the LP-pulvinar might modulate visual responses driven by primary visual cortex or superior colliculus.


Lateral posterior Pulvinar Retinal projections Thalamus Visual cortex lesion 



This study was supported by CIHR (Canada) operating grants to M.P. and C.C. Part of the salary of C.C. is provided by FRSQ (Québec).


  1. Agarwala S, Petry HM, May JG (1989) Retinal projections in the ground Squirrel (Citellus tridecemlineatus). Vis Neurosci 3:537–549PubMedGoogle Scholar
  2. Angelucci A, Clasca F, Sur M (1996) Anterograde axonal tracing with the subunit B of cholera toxin: a highly sensitive immunohistochemical protocol for revealing fine axonal morphology in adult and neonatal brains. J Neurosci Methods 65:101–112CrossRefPubMedGoogle Scholar
  3. Berman N, Jones EG (1977) A retino-pulvinar projection in the cat. Brain Res 164:237–248CrossRefGoogle Scholar
  4. Boire D, Casanova C, Ptito M (2000) Retinal projections to the lateral posterior-pulvinar in normal and striate cortex ablated cats: a cholera toxin B fragment study. Society for Neuroscience Abstracts 26(1-2): 549.13Google Scholar
  5. Bronchti G, Rado R, Terkel J, Wollberg Z (1991) Retinal projections in the blind mole rat: a WGA-HRP tracing study of natural degeneration. Dev Brain Res 58:159–170CrossRefGoogle Scholar
  6. Bruce LL, Kicliter E (1984) A study of retinal projections in the ground squirrel, (Spermophilus tridecemlineatus) using anterograde transport techniques. P R Health Sci J 3:97–106Google Scholar
  7. Casanova C (2003) Functions of the pulvinar in vision. In: Chalupa LM, Werner JS (eds) The visual neurosciences. MIT Press, Cambridge MA, pp 592–608Google Scholar
  8. Casanova C, Freeman RD, Nordmann JP (1989) Monocular and binocular response properties of cells in the striate-recipient zone of the cat’s lateral posterior-pulvinar complex. J Neurophysiol 62:544–557PubMedGoogle Scholar
  9. Casanova C, Savard T, Darveau S (1997) Contribution of area 17 to cell response in the striate-recipient zone of the cat’s lateral posterior-pulvinar complex. Eur J Neurosci 9:1026–1036PubMedGoogle Scholar
  10. Casanova C, Merabet L, Desaultels A, Minville K (2001) Higher-Order motion processing in the pulvinar. Prog Brain Res 134:71–82CrossRefPubMedGoogle Scholar
  11. Clancy B, Darlington RB, Finlay BL (2001) Translating developmental time across mammalian species. Neuroscience 105:7–17CrossRefPubMedGoogle Scholar
  12. Costa MS, Santee UR, Cavalcante JS, Moraes PR, Santos NP, Britto LR (1999) Retinohypothalamic projections in the common marmoset (Callithrix jacchus): a study using cholera toxin subunit B. J Comp Neurol 415:393–403CrossRefPubMedGoogle Scholar
  13. Crain BJ, Hall WC (1980) The organization of afferents to the lateral posterior nucleus in the golden hamster after different combination of neonatal lesions. J Comp Neurol 193:403–412PubMedGoogle Scholar
  14. Cunningham TJ (1972) Sprouting of the optic projections after cortical lesions. Anat Rec 172:298Google Scholar
  15. Cunningham TJ, Huddelston C, Murray M (1979) Modification of neuron numbers in the visual system of the rat. J Comp Neurol 184:423–434PubMedGoogle Scholar
  16. Dumbrava D, Faubert J, Casanova C (2001) Global motion integration in the cat’s lateral posterior-pulvinar complex. Eur J Neurosci 13:2218–2226CrossRefPubMedGoogle Scholar
  17. Erzumulu RS, Jhaveri S, Schneider GE (1988) Distribution of morphological different axon terminals in the hamster dorsal lateral geniculate nucleus. Brain Res 461:175–181CrossRefPubMedGoogle Scholar
  18. Fite KV, Janusonis S, Foote W, Bengston L (1999) Retinal afferents to the dorsal raphe nucleus in rats and Mongolian gerbils. J Comp Neurol 414:469–484CrossRefPubMedGoogle Scholar
  19. Graybiel AM, Berson DM (1980) Histochemical identification and afferent connections of subdivisions in the lateralis posterior-pulvinar complex and related thalamic nuclei in the cat. Neuroscience 5:1175–1238CrossRefPubMedGoogle Scholar
  20. Guido W, Spear PD, Tong L (1990) Functional compensation in the lateral suprasylvian visual area following bilateral visual cortex damage in kittens. Exp Brain Res 83:219–224PubMedGoogle Scholar
  21. Guillery RW, Geisert EE, Polley EH, Mason CA (1980) An analysis of the retinal afferents to the cat’s interlaminar nucleus and to its rostral extension the “geniculate wing”. J Comp Neurol 194:117–142PubMedGoogle Scholar
  22. Harman AM, Coleman LA, Beazley LD (1990) Retinofugal projections in a marsupial, Tarsipes rostratus (Honey possum). Brain Behav Evol 36:30–38PubMedGoogle Scholar
  23. Hutchins B, Updyke BV (1989) Retinotopic organization within the lateral posterior complex of the cat. J Comp Neurol 285:359–398Google Scholar
  24. Itoh K, Mizuno N, Kudo M (1983) Direct retinal projections to the lateroposterior and pulvinar nuclear complex (LP-Pul) in the cat, as revealed by anterograde HRP method. Brain Res 276:325–328CrossRefPubMedGoogle Scholar
  25. Kawamura S, Fukushima N, Hattori S (1979) Topographical origin and ganglion cell type of the retino-pulvinar projection in the cat. Brain Res 173:419–429CrossRefPubMedGoogle Scholar
  26. Kudo M, Nakamura Y, Moriizumi T, Tokuno H, Kitao Y (1988) Direct retinal projections to the lateroposterior thalamic nucleus (LP) in the mole. Neurosci Lett 93:176–180CrossRefPubMedGoogle Scholar
  27. Ling C, Schneider GE, Northmore D, Jhaveri S (1997a) Afferents from the colliculus, cortex and retina have distinct terminal morphologies in the lateral posterior thalamic nucleus. J Comp Neurol 388:467–483CrossRefPubMedGoogle Scholar
  28. Ling C, Jhaveri S, Schneider GE (1997b) Target- as well as source-derived factors direct the morphogenesis of anomalous retino-thalamic projections. J Comp Neurol 388:454–466CrossRefPubMedGoogle Scholar
  29. Ling C, Schneider GE, Jhaveri S (1998) Target-specific morphology of retinal axon arbors in the adult hamster. Vis Neurosci 15:559–579CrossRefPubMedGoogle Scholar
  30. Lomber SG, MacNeil MA, Payne BR (1995) Amplification of thalamic projections to middle suprasylvian cortex following ablation of immature primary visual cortex in cat. Cereb Cortex 2:166–191Google Scholar
  31. Major DE, Rodman HR, Libedinsky C, Karten HJ (2003) Pattern of retinal projections in the California ground squirrel (Spermophilus beecheyi): anterograde tracing study using cholera toxin. J Comp Neurol 463:317–340CrossRefPubMedGoogle Scholar
  32. Mason R (1981) Differential responsiveness of cells in the visual zones of the cat’s LP-pulvinar complex to visual stimuli. Exp Brain Res 43:25–33PubMedGoogle Scholar
  33. Merabet L, Desautels A, Minville K, Casanova C (1998) Motion integration in a thalamic visual nucleus. Nature 396:265–268CrossRefPubMedGoogle Scholar
  34. Mikkelsen JD (1992) Visualization of efferent retinal projections by immunohistochemical identification of cholera toxin subunit B. Brain Res Bull 28:619–623CrossRefPubMedGoogle Scholar
  35. Murphy EH, Grigonis AM, Hayden TE, Tashayyod D, Wilkes M (1988) The effect of ablation of visual cortex in neonatal rabbits on the organization of retinothalamic and retinopretectal projections. Dev Brain Res 38:27–35CrossRefGoogle Scholar
  36. Payne BR, Foley HA, Lomber SG (1993) Visual cortex damage-induced growth of retinal axons into the lateral posterior nucleus of the cat. Vis Neurosci 10:747–752PubMedGoogle Scholar
  37. Payne BR, Lomber SG, Macneil MA, Cornwell P (1996) Evidence for greater sight in blindsight following damage of primary visual cortex early in life. Neuropsychologia 34:741–774CrossRefPubMedGoogle Scholar
  38. Payne BR, Lomber SG, Gelston CD (2000) Graded sparing of visually-guided orienting following primary visual cortex ablations within the first postnatal month. Behav Brain Res 117:1–11CrossRefPubMedGoogle Scholar
  39. Perry VH, Cowey A (1979) Changes in retino-fugal pathways following cortical and tectal lesions in neonatal and adult rats. Exp Brain Res 35:97–108PubMedGoogle Scholar
  40. Reiner A, Zhang D, Eldred WD (1996) Use of the sensitive anterograde tracer cholera toxin fragment B reveals new details of the central retinal projections in turtles. Brain Behav Evol 48:307–337PubMedGoogle Scholar
  41. Reinoso-Suárez F (1961) Topographischer Hirnatlas der Katze. Herausgegeben von E Merck AG, DarmstadtGoogle Scholar
  42. Restrepo CE, Manger PR, Innocenti GM (2002) Retinofugal projections following early lesions of the visual cortex in the ferret. Eur J Neurosci 16:1713–1719CrossRefPubMedGoogle Scholar
  43. Reuss S, Fuchs E (2000) Anterograde tracing of retinal afferents to the tree shrew hypothalamus and raphe. Brain Res 874:66–74CrossRefPubMedGoogle Scholar
  44. Royce GJ, Ward JP, Harting JK (1977) Retinofugal pathways in two marsupials. J Comp Neurol 170:391–414Google Scholar
  45. Sanderson KJ, Pearson LJ (1977) Retinal projections in the Tasmanian devil, Sarcophilus harrisii. J Comp Neurol 188:335–345Google Scholar
  46. Sanderson KJ, Pearson LJ, Haight JR (1979) Retinal projections in the native cat, Dasyurus viverrinus. J Comp Neurol 174:347–357Google Scholar
  47. Schneider GE (1970) Mechanisms of functional recovery following lesions of visual cortex or superior colliculus in neonate and adult hamsters. Brain Behav Evol 3:295–323PubMedGoogle Scholar
  48. Sherman SM, Guillery RW (2001) Exploring the thalamus. Academic Press, San DiegoGoogle Scholar
  49. Shimizu T, Cox K, Karten HJ, Britto LR (1994) Cholera toxin mapping of retinal projections in pigeons (Columbia livia), with emphasis on retinohypothalamic connections. Vis Neurosci 11:441–446PubMedGoogle Scholar
  50. Spear PD, Kalil R, Tong L (1980) Functional compensation in lateral suprasylvian visual area following neonatal visual cortex removal in cats. J Neurophysiol 43:851–869PubMedGoogle Scholar
  51. Takahashi ES, Hickey TL, Oyster CW (1977) Retinogeniculate projections in the rabbit: an autoradiographic study. J Comp Neurol 175:1–12PubMedGoogle Scholar
  52. Tong L, Kalil R, Spear PD (1984) Critical periods for functional and anatomical compensation in the lateral suprasylvian visual area following removal of visual cortex in cats. J Neurophysiol 52:941–960PubMedGoogle Scholar
  53. Updyke BV (1977) Topographic organization of the projections from cortical areas 17, 18 and 19 onto the thalamus, pretectum and superior colliculus in the cat. J Comp Neurol 173:81–122PubMedGoogle Scholar
  54. Uchida K, Mizuno N, Sugimoto T, Itoh K (1982) Autoradiographic demonstration of retinal projections to the brain stem structures in the rabbit using transneuronal tracing technique with special reference to the retinal projections to the inferior olive. Exp Neurol 78:369–379CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Denis Boire
    • 1
  • Isabelle Matteau
    • 2
  • Christian Casanova
    • 1
  • Maurice Ptito
    • 1
  1. 1.École d’OptométrieUniversité de MontréalMontréalCanada
  2. 2.Département de PsychologieUniversité de MontréalCanada

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