European Food Research and Technology

, Volume 213, Issue 3, pp 234–239 | Cite as

Response surface methodology for optimization of growth parameters for the production of carotenoids by a mutant strain of Rhodotorula gracilis

  • Govindaswamy Vijayalakshmi
  • Basavanna Shobha
  • Vasudeva Vanajakshi
  • Soundar Divakar
  • Balaraman Manohar
Original Paper

Abstract.

Response surface methodological studies of growth parameters for carotenoid production by a mutant strain of Rhodotorula gracilis (CFR 0–1) was carried out by a two-level fractional factorial design involving five variables, namely glucose concentration (%), incubation period (days), volume of inoculum (ml/100 ml), pH, and temperature (°C). The response equation developed indicated linear relationship between period of incubation, temperature, sugar concentration, and volume of inoculum on carotenoid production. A temperature of 24 °C was found to exhibit maximum carotenoid production in the mutant. The results indicated maximum carotenogenesis (0.09%) at 8% glucose level, with pH 7.5 and 6.0 ml/100 ml of inoculum for an incubation period of 12 days at 24 °C, which was also verified by experimental data.

Carotenoid production Mutant Rhodotorula gracilis Growth parameter Response surface methodology 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Govindaswamy Vijayalakshmi
    • 1
  • Basavanna Shobha
    • 1
  • Vasudeva Vanajakshi
    • 1
  • Soundar Divakar
    • 2
  • Balaraman Manohar
    • 3
  1. 1.Department of Food Microbiology, Central Food Technological Research Institute, Mysore 570 013 India
  2. 2.Fermentation Technology and Bioengineering, Central Food Technological Research Institute, Mysore 570 013 India
  3. 3.Department of Food Engineering, Central Food Technological Research Institute, Mysore 570 013 India

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