Identification and absolute quantification of animal blood products by peptide markers using an UPLC–MS/MS method
- 31 Downloads
Animal blood products, also known as "liquid meat" or “blood tofu”, are rich in many nutrients, consumed in large quantities, and plagued by adulteration. In this study, a qualitative and absolute quantitative method for blood tofu of different animal species, including pig, bovine, sheep, chicken, and duck was established. Species-specific peptide markers were screened using Proteome Discoverer software after data analysis by high-resolution mass spectrometry. Then, calibration and quantitative analysis were used to identify peptides which could be used for accurate quantification. Finally, three peptides of each species were selected for accurate qualitative and quantitative analyses. The linear correlation coefficients were all greater than 0.995, and the deviation of the quantitative results was 80–120%. The method can directly quantify the accurate blood content in blood tofu.
KeywordsAbsolute quantification Peptide marker Mass spectrometry Blood product Adulteration
This work was supported by “Research and application of key technologies for authenticity detection of important foods” (2017YFC1601700) funded by China Ministry of Science and Technology.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Compliance with ethics requirements
This article does not contain any studies with human or animal subjects.
- 2.Zvereva EA, Kovalev LI, Ivanov AV, Kovaleva MA, Zherdev AV, Shishkin SS, Lisitsyn AB, Chernukha IM, Dzantiev BB (2015) Enzyme immunoassay and proteomic characterization of troponin I as a marker of mammalian muscle compounds in raw meat and some meat products. Meat Sci 105:46–52. https://doi.org/10.1016/j.meatsci.2015.03.001 CrossRefPubMedGoogle Scholar
- 6.Shehata HR, Li J, Chen S, Redda H, Cheng S, Tabujara N, Li H, Warriner K, Hanner R (2017) Droplet digital polymerase chain reaction (ddPCR) assays integrated with an internal control for quantification of bovine, porcine, chicken and turkey species in food and feed. PLoS ONE 12(8):1–17. https://doi.org/10.1371/journal.pone.0182872 CrossRefGoogle Scholar
- 7.Hossain MA, Ali ME, Abd Hamid SB, Asing MS, Mohd Desa MN, Zaidul IS (2016) Double gene targeting multiplex polymerase chain reaction-restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products. J Agric Food Chem 64(32):6343–6354. https://doi.org/10.1021/acs.jafc.6b02224 CrossRefPubMedGoogle Scholar
- 15.Lu W, Liu J, Gao B, Lv X, Yu LL (2017) Technical note: nontargeted detection of adulterated plant proteins in raw milk by UPLC-quadrupole time-of-flight-mass spectrometric proteomics combined with chemometrics. J Dairy Sci 100(9):6980–6987. https://doi.org/10.3168/jds.2017-12574 CrossRefPubMedGoogle Scholar
- 23.Prandi B, Varani M, Faccini A, Lambertini F, Suman M, Leporati A, Tedeschi T, Sforza S (2019) Species specific marker peptides for meat authenticity assessment: a multispecies quantitative approach applied to Bolognese sauce. Food Control 97:15–24. https://doi.org/10.1016/j.foodcont.2018.10.016 CrossRefGoogle Scholar
- 27.Lecrenier MC, Planque M, Dieu M, Veys P, Saegerman C, Gillard N, Baeten V (2018) A mass spectrometry method for sensitive, specific and simultaneous detection of bovine blood meal, blood products and milk products in compound feed. Food Chem 245:981–988. https://doi.org/10.1016/j.foodchem.2017.11.074 CrossRefPubMedGoogle Scholar
- 28.Niedzwiecka A, Boucharef L, Hahn S, Zarske M, Steinhilber A, Poetz O, Zagon J, Seidler T, Braeuning A, Lampen A (2019) A novel antibody-based enrichment and mass spectrometry approach for the detection of species-specific blood peptides in feed matrices (accepted). Food Control. https://doi.org/10.1016/j.foodcont.2018.11.036 CrossRefGoogle Scholar