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Identification and absolute quantification of animal blood products by peptide markers using an UPLC–MS/MS method

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Abstract

Animal blood products, also known as "liquid meat" or “blood tofu”, are rich in many nutrients, consumed in large quantities, and plagued by adulteration. In this study, a qualitative and absolute quantitative method for blood tofu of different animal species, including pig, bovine, sheep, chicken, and duck was established. Species-specific peptide markers were screened using Proteome Discoverer software after data analysis by high-resolution mass spectrometry. Then, calibration and quantitative analysis were used to identify peptides which could be used for accurate quantification. Finally, three peptides of each species were selected for accurate qualitative and quantitative analyses. The linear correlation coefficients were all greater than 0.995, and the deviation of the quantitative results was 80–120%. The method can directly quantify the accurate blood content in blood tofu.

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Acknowledgements

This work was supported by “Research and application of key technologies for authenticity detection of important foods” (2017YFC1601700) funded by China Ministry of Science and Technology.

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Authors and Affiliations

  1. China Meat Research Center, Beijing, 100068, China

    Yingying Zhang, Shouwei Wang, Yanhong Ma, Huichen Li & Yingying Li

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  1. Yingying Zhang
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  2. Shouwei Wang
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  3. Yanhong Ma
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  4. Huichen Li
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Correspondence to Yingying Li.

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Zhang, Y., Wang, S., Ma, Y. et al. Identification and absolute quantification of animal blood products by peptide markers using an UPLC–MS/MS method. Eur Food Res Technol 246, 581–589 (2020). https://doi.org/10.1007/s00217-019-03421-x

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  • DOI: https://doi.org/10.1007/s00217-019-03421-x

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