Innovative techniques for identifying a mechanically separated meat: sample irradiation coupled to electronic spin resonance
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As reported by European Food Safety Authority, the identification of a meat product obtained from mechanically separated meat (MSM) has significance from a point of view both of food quality and safety. As a consequence, it recommended the development of innovative methods for identifying a MSM. The electron spin resonance (ESR) spectroscopy is a useful tool for identifying previously irradiated meat products containing bone. Due to the presence of bone fragments and periosteum (bone skin) in MSM, this technique was exploited for developing an innovative analytical method for identifying a MSM and quantifying bone fragments. This procedure is based on sample irradiation at 1 kGy, after proper sample pre-treatment, followed by analysis by ESR spectroscopy. This analytical method was validated following a validation protocol developed “in-house”. The presence of bone fragments, due to MSM addition in the product formulation, lead to the identification of six characteristic signals on the ESR spectrum. Among these, three signals were always verified in all commercial samples analysed (wurstel), composed of different percentages of MSM. The quantitative determination of bone fragments was also studied through method validation. Good analytical performances were obtained in terms of selectivity, with no interfering signals; sensitivity, with LOD and LOQ equal to 16 and 48 mg/100 g (w/w f.w.), respectively; accuracy, as mean CV % = 12.8% and mean error % = 9%; (n = 18), ruggedness (method applicable to different types of meat products), and measurement uncertainty of 14.6%, confirming that the procedure can be developed successfully for accurate qualitative–quantitative analysis.
KeywordsMechanically separated meat Food irradiation Electronic spin resonance Meat quality Food safety Validation
This work was supported by the Italian Ministry of Health who funded the Project code GR-2013-02358862. Ms. Mariagrazia Russo and Mr. Michele Nicolini (Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy) are gratefully acknowledged for technical assistance.
This work was funded by the Italian Ministry of Health (Grant number GR-2013-02358862).
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Conflict of interest
The authors declare that they have no conflict of interest.
Compliance with ethics requirements
This article does not contain any studies with human participants or animals performed by any of the authors.
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