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Characterization of polyphenolic constituents from Sanguisorba officinalis L. and its antibacterial activity

  • Hong-lin Zhu
  • Gang ChenEmail author
  • Sun-ni Chen
  • Qi-rui Wang
  • Ling Wan
  • Su-ping Jian
Original Paper
  • 66 Downloads

Abstract

Sanguisorba officinalis L., one kind of perennial plants, has been widely distributed in southern Europe, northern Africa and China. The objective of the present work was to evaluate the antibacterial captivities of polyphenolic extract (PE) of S. officinalis L. on five pathogenic bacteria, including Gram-negative bacteria (Escherichia coli and Salmonella typhimurium) and Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Bacillus subtilis). The antibacterial activities were determined by the diameter of inhibition zone, minimum inhibitory concentration and minimum bactericidal concentration tests. The results showed that purified PE had significantly better performance in inhibiting bacteria than crude PE (P < 0.05), and PE had better inhibition effect on Gram-positive bacteria than Gram-negative bacteria. Using LC–ESI–QTOF–MS/MS technology, a total of 44 polyphenolic compounds were tentatively identified, 26 of which have been discussed for the first time in S. officinalis L. Gallic acid, ellagic acid, catechin and their derivatives, which have been identified as antibacterial bioactivities previously, were the major constituents with the amounts of 10, 8 and 11, respectively. Besides, compared with potassium sorbate and sodium benzoate, purified PE with a low concentration had significantly stronger antibacterial ability against all the tested bacteria (P < 0.05), suggesting that purified PE of S. officinalis L. could be a promising source of food preservatives.

Keywords

Sanguisorba officinalis L. Polyphenolic extract Antibacterial ability LC–ESI–QTOF–MS/MS 

Notes

Acknowledgements

We offer our special thanks to Qi-ying Mai and Jing-fang Li for the support of skillful technology about LC–MS, to Long Chen and Hui Xue for the technical assistance with microbiology, and to Ying-xue Du and Juan Li for revising manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interest.

Compliance with ethics requirements

This research does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Hong-lin Zhu
    • 1
  • Gang Chen
    • 1
    Email author
  • Sun-ni Chen
    • 1
  • Qi-rui Wang
    • 1
  • Ling Wan
    • 1
  • Su-ping Jian
    • 1
  1. 1.State Key Laboratory of Food Science and TechnologyNanchang UniversityNanchangChina

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