European Food Research and Technology

, Volume 245, Issue 1, pp 243–255 | Cite as

Purification and identification of dipeptidyl peptidase IV and angiotensin-converting enzyme inhibitory peptides from silver carp (Hypophthalmichthys molitrix) muscle hydrolysate

  • Yuqi Zhang
  • Huaigao Liu
  • Hui Hong
  • Yongkang LuoEmail author
Original Paper


Diabetes and hypertension are increasing threats to human health, which could be improved by inhibiting dipeptidyl peptidase IV (DPP-IV) and angiotensin-converting enzyme (ACE), respectively. Herein, we used five different enzymes to hydrolyze silver carp (Hypophthalmichthys molitrix) muscle protein. Among all treatments, the Neutrase-generated hydrolysate that was purified by a three-step isolation and then was identified using liquid chromatography–tandem mass spectrometry resulted in the most potent DPP-IV inhibitory activity and relatively high ACE inhibitory activity. The identified peptide Ala-Ala-Leu-Glu-Gln-Thr-Glu-Arg was the most effective at inhibiting DPP-IV with a half-maximal inhibitory concentration (IC50) value of 647.02 ± 4.30 µM. Leu-Leu-Asp-Leu-Gly-Val-Pro showed the highest ACE inhibitory activity with an IC50 value of 329.33 ± 15.53 µM. Lys-Ala-Val-Gly-Glu-Pro-Pro-Leu-Phe exhibited the dual inhibition against both DPP-IV and ACE with IC50 values of 1,317.39 ± 20.93 µM and 726.01 ± 8.69 µM, respectively. Interestingly, it seemed that the majority of peptides which were responsible for inhibiting DPP-IV were different from the ones which caused the inhibition of ACE.


Sliver carp Dipeptidyl peptidase IV Angiotensin converting enzyme Dual inhibition 



This study was supported by the National Science and Technology Ministry of China (Award number 2017YFD0400200) and the China Agriculture Research System (CARS-45).

Compliance with ethical standards

Conflict of interest

All author declares that they have no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yuqi Zhang
    • 1
  • Huaigao Liu
    • 2
  • Hui Hong
    • 1
    • 3
  • Yongkang Luo
    • 1
    Email author
  1. 1.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.Beijing Guotai Biotechnology Co., LtdBeijingChina
  3. 3.Department of Agricultural, Food and Nutritional ScienceUniversity of AlbertaEdmontonCanada

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