European Food Research and Technology

, Volume 244, Issue 10, pp 1815–1820 | Cite as

An alternative method for rapid and specific authentication of four European Donax species, including D. trunculus a commercially-important bivalve

  • Jenyfer Fernández-PérezEmail author
  • Ana Nantón
  • Josefina Méndez
Original Paper


Seafood fraud or mislabelling has attracted much attention as it is widespread worldwide, and the scientific name of the species must be indicated on commercial products to satisfy labelling and traceability requirements. Species identification using morphological criteria is difficult to non-specialist, and shell-less specimens, processed products or larval and juvenile forms are morphologically unidentifiable. On the other hand, conventional methods, such as PCR-RFLPs, are tedious, expensive and time-consuming. As an alternative, a multiplex PCR of two rDNA regions, containing the 5S and the ITS, was developed for the identification of the four European Donax species: D. semistriatus, D. trunculus, D. variegatus and D. vittatus. The species-specific electrophoretic profiles allowed their identification in an efficient, fast, simple and low-cost method, offering a feasible application in aquaculture. These genetic markers can be very useful for traceability of the species, application in the management of wedge clam cultures, and conservation of these resources.


Donax species Mislabelling Multiplex PCR Species authentication Wedge clams 



We would like to thank Dra. L. Martínez for her constructive and valuable comments. We also thank two anonymous referees for useful comments during manuscript review. This work was supported by the Ministerio de Economía y Competitividad (Spain) through project AGL2016-75288-R AEI/FEDER, UE.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jenyfer Fernández-Pérez
    • 1
    Email author
  • Ana Nantón
    • 1
  • Josefina Méndez
    • 1
  1. 1.Grupo Xenomar, Departamento de Bioloxía, Facultade de Ciencias and Centro de Investigaciones Científicas Avanzadas (CICA)Universidade da CoruñaA CoruñaSpain

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