European Food Research and Technology

, Volume 245, Issue 3, pp 545–558 | Cite as

Processing of brewing by-products to give food ingredient streams

  • Matias Falk Bjerregaard
  • Angelos Charalampidis
  • Rasmus Frøding
  • Radhakrishna Shetty
  • Helena Pastell
  • Charlotte Jacobsen
  • Shiwen Zhuang
  • Manuel Pinelo
  • Preben Bøje Hansen
  • Timothy John HobleyEmail author
Original paper


Very large amounts of brewer’s spent grains (BSG) are produced in the world which is usually considered as a waste, or animal feed, rather than food for humans. Here, we report, for the first time, a new process at pilot scale for the separation of brewer’s spent grain and trub to solid and liquid streams that can be used in foods. A new type of continuous rotary drum press was used to process hot BSG to produce a liquid filtrate and a filter cake stream. Analysis showed that of the starting mass of BSG (ca. 120 kg), the liquid filtrate composed 50% of the mass, and the filter cake fraction composed 50% of the mass. The dry weight (DW) content of the BSG increased from 23 to over 35%. This led to concentration of insoluble dietary fibre (from 38 to 54%) and phenolics in the filter cake (from 102 to 150 mg/100 g DW as gallic acid equivalents). No fractionation of soluble species such as proteins occurred. Centrifugation of the filtrate from the rotary drum press led to a clarified supernatant stream and a paste. Concentration of insoluble dietary fibre and phenolics occurred in the paste (from 5 to 14% of DW and 61 to 114 mg/100 g DW as gallic acid equivalents), whereas soluble fibre and protein did not selectively partition. Given that the unit operations used here are scaleable and approved for food production, an industrially feasible route now exists to process brewers spent grains to ingredients.


Brewer’s spent grains Filter press Pilot scale Trub Separation 



We thank Hening-Holck Larsen and Novozymes foundation for a scholarship til Radhakrishna Shetty. We acknowledge the support of Groen Omstillingsfond project number 2014–98907. We thank Heidi Olander Petersen and Inge Holmberg for excellent technical assistance on Kjeldahl, Dumas, and antioxidant measurements. We thank Per Hägglund for advice on mass spectrometry measurements.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.

Supplementary material

217_2018_3224_MOESM1_ESM.docx (62 kb)
Supplementary material 1 (DOCX 61 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.National Institute for FoodTechnical University DenmarkKongens LyngbyDenmark
  2. 2.Department of Chemical EngineeringTechnical University DenmarkKongens LyngbyDenmark
  3. 3.Finnish Food Safety Authority Evira, Chemistry and Toxicology Research UnitHelsinkiFinland
  4. 4.Dacofi ApSKongens LyngbyDenmark
  5. 5.Carlsberg Research LaboratoryCopenhagenDenmark

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