Development and validation of a novel method for aroma dilution analysis by means of stir bar sorptive extraction
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Stir bar sorptive extraction (SBSE) coupled with gas chromatography–mass spectrometry–olfactometry (GC–MS–O) allows for isolation and identification of aroma compounds in minimal sample preparation time. By means of this extraction technique in direct sample immersion, an independent approach of aroma dilution analysis (ADA) for the rapid determination of flavor dilution (FD) factors was developed based on dilution of the carrier gas flow. The used gas chromatography system equipped with a thermal desorption unit (TDU) and a cooled injection system (CIS) allowed to independently split the gas flow during thermodesorption and sample injection, respectively. The resulting overall split ratio ratios corresponded to the dilution factors. The developed method based on combining both splitting options allowed to determine FD factors in the range from 4 to 8192 in a reliable and reproducible manner. Validation of the method was performed with an aqueous solution containing 12 authentic standards, and a high linearity was confirmed for the binary logarithmic relationship between the peak area and the overall split ratio.
KeywordsStir bar sorptive extraction Olfactometry Aroma dilution analysis Thermodesorption Cryo-focusing Gas chromatography
Aroma dilution analysis
Aroma extract dilution analysis
Cooled injection system
Olfactory detection port
Relative standard deviation
Solvent-assisted flavor evaporation
Stir bar sorptive extraction
Selected ion monitoring
Thermal desorption unit
This study was financially supported by the excellence initiative of the Hessian Ministry of Science and Art which encompasses generous grants for the LOEWE centre “Insect Biotechnology and Bioresources” and “HessenModellProjekte” (Project No. 478/15-20).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Compliance with ethics requirements
This article does not contain any studies with human or animal subject.
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