European Food Research and Technology

, Volume 244, Issue 5, pp 949–957 | Cite as

Development and validation of a novel method for aroma dilution analysis by means of stir bar sorptive extraction

  • Tobias Trapp
  • Deborah A. Jäger
  • Marco A. Fraatz
  • Holger Zorn
Short Communication
  • 122 Downloads

Abstract

Stir bar sorptive extraction (SBSE) coupled with gas chromatography–mass spectrometry–olfactometry (GC–MS–O) allows for isolation and identification of aroma compounds in minimal sample preparation time. By means of this extraction technique in direct sample immersion, an independent approach of aroma dilution analysis (ADA) for the rapid determination of flavor dilution (FD) factors was developed based on dilution of the carrier gas flow. The used gas chromatography system equipped with a thermal desorption unit (TDU) and a cooled injection system (CIS) allowed to independently split the gas flow during thermodesorption and sample injection, respectively. The resulting overall split ratio ratios corresponded to the dilution factors. The developed method based on combining both splitting options allowed to determine FD factors in the range from 4 to 8192 in a reliable and reproducible manner. Validation of the method was performed with an aqueous solution containing 12 authentic standards, and a high linearity was confirmed for the binary logarithmic relationship between the peak area and the overall split ratio.

Keywords

Stir bar sorptive extraction Olfactometry Aroma dilution analysis Thermodesorption Cryo-focusing Gas chromatography 

Abbreviations

ADA

Aroma dilution analysis

AEDA

Aroma extract dilution analysis

CIS

Cooled injection system

DI

Direct immersion

FD

Flavor dilution

GC

Gas chromatography

HS

Headspace

MS

Mass spectrometry

O

Olfactometry

ODP

Olfactory detection port

RSTD

Relative standard deviation

SAFE

Solvent-assisted flavor evaporation

SBSE

Stir bar sorptive extraction

SDE

Simultaneous distillation–extraction

SIM

Selected ion monitoring

SPME

Solid-phase microextraction

SR

Split ratio

TDU

Thermal desorption unit

Notes

Acknowledgements

This study was financially supported by the excellence initiative of the Hessian Ministry of Science and Art which encompasses generous grants for the LOEWE centre “Insect Biotechnology and Bioresources” and “HessenModellProjekte” (Project No. 478/15-20).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subject.

Supplementary material

217_2017_3003_MOESM1_ESM.pdf (198 kb)
Supplementary material 1 (PDF 197 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Institute of Food Chemistry and Food BiotechnologyJustus Liebig University GiessenGiessenGermany

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