Molecular characterization of Dalmatian cultivars and the influence of the olive fruit harvest period on chemical profile, sensory characteristics and oil oxidative stability
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Four Dalmatian autochthonous olive cultivars (Buhavica, Drobnica, Lastovka and Oblica) were molecularly characterized by analyzing length variability of genomic DNA sequences encompassing 15 microsatellite repeats. Furthermore, several important parameters of olive oils were analyzed in relation to the harvest period. An analysis of major phenolics secoiridoids was done by qNMR, while the fatty acid profile of oils and squalene content was determined by GC–FID. Oxidative stability was evaluated by the Rancimat method and sensory evaluation was carried out by a trained professional panel. The results indicate that the effect of the harvest period on the phenolic profile of oils depends on the olive cultivar and is related to its genetic profile. Drobnica oil from the late harvest contained an extremely high concentration of oleocanthal + oleacein (966 mg/kg). The longest oxidative stability was achieved by Drobnica and Lastovka oils from the early harvest period (20.95 and 18.65 h). Squalene had no effect on the oil oxidative stability. This study shows that the content of phenolic secoiridoids depends mainly on the cultivar. In addition, some cultivars, such as Drobnica did not show significant change of phenolic secoiridoids content in relation to the harvest period.
KeywordsMonovarietal olive oil Secoiridoids Fatty acids Rancimat Sensory evaluation Molecular characterization
This work was supported by Grant 201600066739 (April 2016) from the Split-Dalmatia County of the Republic of Croatia (SDC Agricultural Development Fund).
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Conflict of interest
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This article does not contain any studies with human or animal subject.
- 1.Boskou D (2015) Olive and olive oil bioactive constituents. AOCS Press, Illinois, pp 2–11Google Scholar
- 7.Mansour AB, Flamini G, Ben Selma Z, Le Dréau Y, Artaud J, Abdelhedi R, Bouaziz M (2015) Olive oil quality is strongly affected by cultivar, maturity index and fruit part: Chemometrical analysis of volatiles, fatty acids, squalene and quality parameters from whole fruit, pulp and seed oils of two Tunisian olive cultivars. Eur J Lipid Sci Technol 117:976–987CrossRefGoogle Scholar
- 9.CBS-Croatian Bureau of Statistic (2016) Production of vegetables, fruits and grapes, 2015—Previous Data, 2013, No. 1.1.31Google Scholar
- 10.IOC-International Olive Oil Councile (2016) Market newsletter, No 110, November 2016, pp 1–6Google Scholar
- 11.Štambuk S, Sutlović D, Bakarić P, Petričević S, Anđelinović S (2007) Forensic botany: potential usefulness of microsatellite-based genotyping of Croatian olive (Olea europaea L.) in forensic casework. Croat Med J 48(4):556–562Google Scholar
- 13.Ercisli S, Bencic D, Ipek A, Barut E, Liber Z (2012) Genetic relationship among olive (Olea europaea L.) cultivars native to Croatia and Turkey. J Appl Bot Food Qual 85:144–149Google Scholar
- 14.Brkić-Bubola K, Krapac M, Lukić I, Sladonja B, Autino A, Cantini C, Poljuha D (2014) Morphological and molecular characterization of Bova olive cultivar and aroma fingerprint of its oil. Food Technol Biotechnol 52(3):342–350Google Scholar
- 15.Žanetić M, Štrucelj D, Perica S, Rade D, Škevin D, Serraiocco A, Simone N (2010) Chemical composition of Dalmatian virgin olive oils from autochtonous olive cultivars Oblica, Lastovka and Levantinka. Riv Ital Sostanze Grasse 87(1):24–33Google Scholar
- 16.Žanetić M, Cerretani L, Škevin D, Politeo O, Vitanović E, Jukić Špika M, Perica S, Ožić M (2013) Influence of polyphenolic compounds on the oxidative stability of virgin olive oils from selected autochthonous varieties. J Food Agric Environ 11(1):126–131Google Scholar
- 17.Šarolić M, Gugić M, Tuberoso CIG, Jerković I, Šuste M, Marijanović Z, Marek Kuś P (2014) Volatile profile, phytochemicals and antioxidant activity of virgin olive oils from Croatian autochthonous varieties Mašnjača and Krvavica in comparison with Italian variety Leccino. Molecules 19:881–895CrossRefGoogle Scholar
- 24.Diamantakos P, Velkou A, Killday BK, Gimisis T, Melliou E, Magiatis P (2015) Oleokoronal and oleomissional: new major phenolic ingredients of extra virgin olive oil. Olivae 122:22–35Google Scholar
- 25.IOC—International Olive Oil Councile (2015) Organoleptic assessment of virgin olive oil, COI/T.20/Doc. No 15/Rev. 8Google Scholar
- 26.European Community, Commission Regulation – EEC (1991) No. 2568/91 on the characteristics of olive oil and olive residue oil and on the relevant methods of analysis. Annex XII: organoleptic assessment of virgin olive oil. Off J Eur Commun L248:1–83Google Scholar
- 27.Bakarić P (1995) The olives from the island of Korčula. Blatski ljetopis, pp 69–84 (Elajografija otoka Korčule)Google Scholar
- 32.Li YC, Korol AB, Fahima T, Nevo E (2004) Microsatellites within genes: structure, function, and evolution. Mol Ecol Notes 21(6):991–1007Google Scholar