This work aims to study the effect of processing on the concentration of TGF-β2, evaluating pasteurization, membrane separation, as well as vacuum evaporation and spray drying. The TGF-β content in each sample of milk taken after each processing step has been quantified by ELISA kit. Furthermore, chemical composition analysis has been performed. TGF-β2 has shown a strong correlation (0.95) with the protein content in the samples. There is no influence of pasteurization on the concentration of TGF-β2, although pasteurization affects the distribution of TGF-β2 in the whey protein fractions and casein, which can be observed in the reduction of TGF-β2 content in whey as heat treatment is applied. This trend is unlike that of microfiltration, where it has been observed that TGF-β2 was equally distributed between casein and whey proteins. The concentration obtained by vacuum evaporation showed a concentration factor less than 1 for TGF-β2 once the constituents other than TGF-β2 became more concentrated. However, spray drying showed values greater than 1, demonstrating that TGF-β2 is concentrated in greater quantities than other solid constituents of the samples.
Francis GL, Regester GO, Webb HA, Ballard FJ (1995) Extraction from cheese whey by cation-exchange chromatography of factors that stimulate the growth of mammalian cells. J Dairy Sci 78:1209–1218CrossRefGoogle Scholar
Maubois J, Fauguant J, Jouan P, Bourtourault M (2003) Method for obtaining a TGF-beta enriched protein fraction in activated form, protein fraction and therapeutic applications. Patent US No. 7141262B2Google Scholar
Ginjala V, Pakkanen R (1998) Determination of transforming growth factor-β1 (TGF-β1) and insulin-like growth factor 1 (IGF-1) in bovine colostrum samples. J Immunoass 19:195–207CrossRefGoogle Scholar
Ollikainen P, Muuronen K, Tikanmaki R (2012) Effect of pasteurization on the distribution of bovine milk transforming growth factor-β2 in casein and whey fractions during micro- and ultrafiltration processes. Int Dairy J 26:141–146. doi:10.1016/j.idairyj.2012.04.004CrossRefGoogle Scholar
Bravo FI, Felipe X, López-Fandiño R, Molina E (2013) High-pressure treatment of milk in industrial and pilot-scale equipments: effect of the treatment conditions on the protein distribution in different milk fractions. Eur Food Res Technol 236:499–506. doi:10.1007/s00217-012-1902-9CrossRefGoogle Scholar
Elfstrand L, Lindmark-Mansson H, Paulsson M et al (2002) Immunoglobulins, growth factors and growth hormone in bovine colostrum and the effect of processing. Int Dairy J 12:879–887CrossRefGoogle Scholar
Sgarbieri VC (1996) Proteínas em alimentos proteicos: propriedades, degradações, modificações. Varela, São PauloGoogle Scholar