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European Food Research and Technology

, Volume 242, Issue 7, pp 1177–1185 | Cite as

Interaction between gluten proteins and their mixtures with water-extractable arabinoxylan of wheat by rheological, molecular anisotropy and CP/MAS 13C NMR measurements

  • Fumin Ma
  • Yali DangEmail author
  • Shiying Xu
Original Paper

Abstract

In this study, water-extractable arabinoxylan, WEAX, of wheat was isolated and mixed with gluten to observe small and large strain behavior and microscopic structure of the mixtures. The results showed that WEAX improves the viscoelasticity of gluten and makes the microscopic structure of gluten less compact. Free sulfhydryl, fluorescence anisotropy and CP/MAS 13C NMR measurements were used to gain a better understanding of the interactions between WEAX and gluten proteins of wheat. It was demonstrated that when gluten was mixed with WEAX, the free –SH of gluten proteins cross-linked to each other or to WEAX. The cross-linking happened mainly to LMW-GS. It was shown from the fluorescence anisotropy that the conformation of glutenin was more greatly influenced by WEAX than gliadin because of the difference in their molecular conformation. In addition, it was found in this study that tyrosine groups of glutenin also played an important role in the interactions between WEAX and gluten.

Keywords

Water-extractable arabinoxylan Gluten protein Viscoelasticity Interaction Molecular anisotropy CP/MAS 13C NMR 

Notes

Acknowledgments

Special thanks to Mr. Kongying, Zhu, Analysis and Test Center, the University of Tianjin, for his assistance in CP/MAS 13C NMR measurement of the samples used in this study. And also special thanks to Miss. Ren Wei, Liaoning University of Traditional Chinese Medicine, for her kind assistance in reviewing and amending an earlier manuscript. This work was supported by the National Natural Science Foundation of China under Grant No. 31440068 and by Science Technology Department of Zhejiang Province under Grant No. 2014C02023.

Compliance with ethical standards

Conflict of interest

None.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.

References

  1. 1.
    Don C, Lichtendonk WJ, Plijter JJ, Tv Vliet, Hamer RJ (2005) The effect of mixing on glutenin particle properties: aggregation factors that affect gluten function in dough. J Cereal Sci 41:69–83CrossRefGoogle Scholar
  2. 2.
    Shewry PR, Tatham AS (1997) Disulphide bonds in wheat gluten proteins. J Cereal Sci 25:207–227CrossRefGoogle Scholar
  3. 3.
    Lindsay MP, Skerritt JH (1999) Macropolymer of wheat flour doughs: structure–function perspectives. Trends Food Sci Technol 10:247–253CrossRefGoogle Scholar
  4. 4.
    Carvajal-Millan E, Landillon V, Morel MH, Rouau X, Doublier JL, Micard V (2005) Arabinoxylan gels: impact of the feruloylation degree on their structure and properties. Biomacromolecules 6:309–317CrossRefGoogle Scholar
  5. 5.
    Bunzel M, Allerdings E, Ralph J, Steinhart H (2008) Cross-linking of arabinoxylans via 8-8-coupled diferulates as demonstrated by isolation and identification of diarabinosyl 8-8(cyclic)-dehydrodiferulate from maize bran. J Cereal Sci 1(47):29–40CrossRefGoogle Scholar
  6. 6.
    Fessas D, Schiraldi A (2003) Interactions between arabinoxylans and wheat proteins. Food Chem 23:221–225Google Scholar
  7. 7.
    Michniewics J, Biliaderis CG, Bushuk W (1992) Effect of added pentosans on some properties of wheat bread. Food Chem 43(4):251–257CrossRefGoogle Scholar
  8. 8.
    Courtin CM, Delcour JA (2001) Relative activity of endoxylanases towards water-extractable and water-unextractable arabinoxylan. J Cereal Sci 33:301–312CrossRefGoogle Scholar
  9. 9.
    Wang M, Vliet TV, Hamer RJ (2004) How gluten properties are affected by pentosans. J Cereal Sci 39:395–402CrossRefGoogle Scholar
  10. 10.
    Saulnier L, Andersson A, Åman P (1997) A study of the polysaccharide components in gluten. J Cereal Sci 25:121–127CrossRefGoogle Scholar
  11. 11.
    Fang Y, Chen D (2000) Progress in the photophysical studies of solid/water interface behaviors of water soluble polymers. Polym Bull 6:58–64Google Scholar
  12. 12.
    Fang X, Cao Z, Beck T, Tan W (2001) Molecular aptamer for real-time oncoprotein platelet-derived growth factor monitoring by fluorescence anisotropy. Anal Chem 73:5752–5757CrossRefGoogle Scholar
  13. 13.
    Faurot A-L, Saulnier L, Bérot S, Popineau Y, Petit M-D, Rouau X, Thibault J-F (1995) Large scale isolation of water-soluble and water-insoluble pentosans from wheat flour. Lebensm Wiss Technol 28:436–441CrossRefGoogle Scholar
  14. 14.
    Islas Rubio AR, Singh H, Chittrakorn S, MacRitchie F (2006) Stability of wheat proteins in solution. J Cereal Sci 43:169–174CrossRefGoogle Scholar
  15. 15.
    Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRefGoogle Scholar
  16. 16.
    Egorov TA, Odintsova TI, Shewry PR, Tatham AS (1998) Characterisation of high wheat glutenin polymers by agarose geleletrophoresis and dynamic light scattering. FEBS Lett 434:215–217CrossRefGoogle Scholar
  17. 17.
    Verbruggen IM, Veraverbeke WS, Vandamme A, Delcour JA (1998) Simultaneous isolation of wheat high molecular weight and low molecular weight glutenin subunits. J Cereal Sci 28:25–32CrossRefGoogle Scholar
  18. 18.
    Cummings JH, Macfarlane GT, Englyst HN (2001) Prebiotic digestion and fermentation. Am J Clin Nutr 73(2):415–420Google Scholar
  19. 19.
    Ma F, Xu S, Xu M, Guo X (2012) The influence of water soluble pentosan on viscoelasticity of gluten. J Food Eng 111:166–175CrossRefGoogle Scholar
  20. 20.
    Maeda T, Yamashita H, Morita N (2007) Application of xyloglucan to improve the gluten membrane on breadmaking. Carbohydr Polym 68:658–664CrossRefGoogle Scholar
  21. 21.
    Arnosti C (2003) Fluorescent derivatization of polysaccharides and carbohydrate-contain-ing biopolymers for measurement of enzyme activities in complex media. J Chromatogr B 793:181–191CrossRefGoogle Scholar
  22. 22.
    Rao MA, Cooley HJ (1992) Rheological behavior of tomato pastes in steady and dynamic shear. J Texture Stud 23(4):415–425CrossRefGoogle Scholar
  23. 23.
    Barnes HA (2000) A handbook of elementary rheology. Aberystwyth: institute of non-Newtonian fluid mechanics. Wales, University of Wales, CardiffGoogle Scholar
  24. 24.
    Steffe JF (1996) Rheological methods in food process engineering. Freeman Press, East LansingGoogle Scholar
  25. 25.
    Karaman S, Yilmaz MT, Cankurt H, Kayacier A, Sagdic O (2012) Linear creep and recovery analysis of ketchup–processed cheese mixtures using mechanical simulation models as a function of temperature and concentration. Food Res Int 48(2):507–519CrossRefGoogle Scholar
  26. 26.
    Stathopoulos CE, Tsiami AA, Schofield JD, Dobraszczyk BJ (2008) Effect of heat on rheology, surface hydrophobicity and molecular weight distribution of glutens extracted from flours with different bread-making quality. J Cereal Sci 47(2):134–143CrossRefGoogle Scholar
  27. 27.
    Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer, New YorkCrossRefGoogle Scholar
  28. 28.
    Minussi RC, Pastore GM, Durná N (2002) Potential applications of laccase in the food industry. Trends Food Sci Technol 13:205–216CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities, Education Department of Jilin Provincial GovernmentChangchun UniversityChangchunChina
  2. 2.Institute of Health FoodZhejiang Academy of Medical SciencesHangzhouChina
  3. 3.School of Food Science and TechnologyJiangnan UniversityWuxiChina

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