European Food Research and Technology

, Volume 240, Issue 3, pp 619–625 | Cite as

A new sensitive method for the detection of chloramphenicol in food using time-resolved fluoroimmunoassay

  • Bin Zhou
  • Jue Zhang
  • Jun Fan
  • Lan Zhu
  • Yi Zhang
  • Jian Jin
  • Biao Huang
Original Paper


A time-resolved fluoroimmunoassay (TRFIA) technique was developed to detect chloramphenicol (CAP) contamination in food. By using CAP-ovalbumin, anti-CAP antibodies, and europium-labelled goat anti-rabbit antibodies, an indirect, competitive method for CAP-TRFIA was established. The sensitivity was high, with a detection limit of 0.008 μg/L (8 ppt) for indirect competitive TRFIA formats. Testing showed that the sensitivity of the technique was 2.67 μg/kg in honey, prawn, and chicken muscle tissues, and 0.32 μg/L in milk. The detection range was between 0.008 and 100 μg/L: within this, the intra- and inter-batch coefficients of variation of the CAP-TRFIA method were 6.8 and 13.5 %, respectively. The study suggested that CAP-TRFIA was a simple, sensitive, and cost-effective method of screening large quantities of samples and had good prospects for further application.


Chloramphenicol (CAP) Veterinary drug residues Time-resolved fluoroimmunoassay (TRFIA) Food 


Conflict of interest


Compliance with Ethics Requirements

This article does not contain any studies with human subjects. All institutional and national guidelines for the care and use of laboratory animals were followed.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Bin Zhou
    • 1
  • Jue Zhang
    • 1
  • Jun Fan
    • 1
  • Lan Zhu
    • 1
  • Yi Zhang
    • 1
  • Jian Jin
    • 2
  • Biao Huang
    • 1
  1. 1.Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear MedicineMinistry of HealthWuxiPeople’s Republic of China
  2. 2.School of Medicine and PharmaceuticsSouthern Yangtze UniversityWuxiPeople’s Republic of China

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