European Food Research and Technology

, Volume 238, Issue 4, pp 597–605 | Cite as

Characterization and amino acid metabolism performances of indigenous Oenococcus oeni isolated from Chinese wines

Original Paper


Oenococcus oeni is a multiple physical stress-tolerant lactic acid bacterium that plays an important role in wine making. It is often added as a starter culture to carry out malolactic fermentation (MLF). In this study, a total of 22 out of 127 lactic acid bacteria, isolated from Chinese wines undergoing MLF, were identified as O. oeni by species-specific PCR and 16S rRNA sequencing. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis showed that all strains could be typed under these conditions, and three main groups were determined by cluster analysis, which showed intraspecific homology higher than 69 %. Eight strains, representative of SE-AFLP clusters, were tested for malolactic activity. Significant differences were observed among strains with regard to the amount of malic acid consumed. Seventeen amino acids in different wines that were inoculated by 4 O. oeni strains, respectively, were analyzed before and after MLF. The results indicated that the amino acid metabolism of the 4 strains was significantly different between each strain.


Oenococcus oeni Species-specific PCR 16S rRNA sequences SE-AFLP Amino acid metabolism 


Conflict of interest


Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

Supplementary material

217_2013_2112_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 12 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.College of EnologyNorthwest A&F UniversityYanglingChina
  2. 2.Shaanxi Engineering Research Center for Viti-VinicultureYanglingChina
  3. 3.College of Life ScienceNorthwest A&F UniversityYanglingChina
  4. 4.College of Biology and Brewing EngineeringTaishan UniversityTaianChina
  5. 5.School of Agriculture, Food and WineThe University of AdelaideUrrbraeAustralia

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