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European Food Research and Technology

, Volume 237, Issue 3, pp 377–384 | Cite as

Bleaching of colored whey and milk by a multiple-enzyme system

  • Renata T. Szweda
  • Katharina Schmidt
  • Holger Zorn
Original Paper

Abstract

Carotenoids are broadly used to enhance the color of cheese types like Cheddar and Gouda. While ∼80 % of the colorants are transferred into the cheese, the rest remains in the whey and impedes its commercial utilization. Therefore, cheese whey is currently bleached chemically by addition of either hydrogen peroxide or benzoyl peroxide in the industrial practice. To avoid heat- and peroxide-induced protein denaturation and the formation of off-flavors, an enzymatic bleaching process was developed. The ability of the fungal peroxidase MsP1 to degrade carotenoids was successfully employed for the bleaching of colored whey and milk in two- and three-enzyme systems, respectively. The systems were composed of MsP1, glucose oxidase, and when necessary, acid lactase. The initial step of the three-enzyme system was the lactase-catalyzed hydrolysis of lactose to galactose and glucose. The latter served as a substrate for the enzyme glucose oxidase in the second step which yielded gluconic acid and hydrogen peroxide. Finally, MsP1 oxidatively degraded the carotenoids. The activities of the involved enzymes were fine-tuned to optimize the bleaching process.

Keywords

Peroxidase Basidiomycete Bleaching Annatto β-Carotene 

Notes

Acknowledgments

This work was supported by the excellence initiative of the Hessian Ministry of Science and Art which encompasses a generous grant for the LOEWE research focus ‘Insect Biotechnology.’

Conflict of interest

None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Renata T. Szweda
    • 1
  • Katharina Schmidt
    • 1
  • Holger Zorn
    • 1
  1. 1.Institute of Food Chemistry and Food BiotechnologyJustus Liebig University GiessenGiessenGermany

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