European Food Research and Technology

, Volume 236, Issue 1, pp 145–154 | Cite as

Effects of nutrient supplementation on fermentation kinetics, H2S evolution, and aroma profile in Verdicchio DOC wine production

  • Mirko Gobbi
  • Francesca Comitini
  • Giuliano D’Ignazi
  • Maurizio Ciani
Original Paper


Different yeast nutrient additions were studied for the 2008 and 2009 vintages of Verdicchio grape juice fermentation. Addition of yeast derivatives at the beginning of fermentation and/or different amounts of diammonium phosphate at various times within the first half of fermentation were examined, with initial yeast assimilable nitrogen concentrations set at 200 and 250 mg l−1. Supplementation with glutathione in combination with this nitrogen addition was also evaluated. Fermentation rates were monitored throughout these fermentations carried out under different nutrient conditions. H2S production during fermentation and synthesis of volatile compounds in the finished wines were quantified; the wines also underwent sensory evaluation. The fermentation kinetics were almost exclusively influenced by the inorganic nitrogen supplementation with diammonium phosphate. H2S evolution was more affected by assimilable nitrogen than glutathione. Diammonium phosphate significantly reduced H2S production, with a further reduction in the presence of yeast derivative. This nitrogen supplementation yielded higher concentrations of acetate esters, and in particular of isoamyl acetate (fruity aromas), which positively influences the analytical and aroma profile of wines and results in a general reduction in 2-phenylethanol production (floral aromas). Overall results (two harvesting times and vintages) indicate that the management with diammonium phosphate and yeast derivative supplementation improves the kinetics of fermentation and provides a good tool to reduce H2S formation and increase the analytical and sensory quality of Verdicchio wine.


Diammonium phosphate Yeast derivatives S. cerevisiae H2Aroma profile Verdicchio wine 



The authors wish to thank the winery Terre Cortesi Moncaro s.r.c.l. for financial support as well as V. Durastanti and T. Duca for their analytical and technical assistance in the cellar.


  1. 1.
    Acree TE, Sonoff EP, Spittstoesser DF (1971) Determination of hydrogen sulfide in fermentation broths containing SO2. Appl Microbiol 22:110–112Google Scholar
  2. 2.
    Agenbach WA (1977) A study of must nitrogen content in relation to incomplete fermentations, yeast production and fermentation activity. In: Beukman EF (ed) Proceedings of the South African Society for Enology and Viticulture. Stellenbosch, South Africa, pp 66–88Google Scholar
  3. 3.
    Amerine MA, Berg HW, Kunkee RE, Ough CS, Singleton VL, Webb AD (1980) The composition of grapes. In: Amerine MA, Berg HW, Kunkee RE, Ough CS, Singleton VL, Webb AD (eds) The technology of wine making, 4th edn. AVI Publishing Company, WestportGoogle Scholar
  4. 4.
    Bely M, Salmon JM, Barre P (1994) Assimilable nitrogen addition and hexose transport system activity during enological fermentation. J Inst Brew 100:279–282Google Scholar
  5. 5.
    Bely M, Sablayrolles J, Barre P (1990) Description of alcoholic fermentation kinetics—its variability and significance. Am J Enol Vitic 41:319–324Google Scholar
  6. 6.
    Bezenger MC, Navarro JM (1988) Alcoholic fermentation: model accounting for initial nitrogen influence. Biotechnol Bioeng 31:747–749CrossRefGoogle Scholar
  7. 7.
    Bisson LF (1991) Influence of nitrogen on yeast and fermentation of grapes. In: Proceedings of the international symposium on nitrogen in grapes and wine, Seattle, USA. American Society for Enology and Viticulture, Davis, pp 78–89 and 266–269Google Scholar
  8. 8.
    Bisson LF, Butzke CE (2000) Diagnosis and rectification of stuck and sluggish fermentations. Am J Enol Vitic 51:168–177Google Scholar
  9. 9.
    Comuzzo P, Tat L, Tonizzo A, Battistutta F (2006) Yeast derivatives (extracts and autolysates) in winemaking: release of volatile compounds and effects on wine aroma volatility. Food Chem 99:217–230CrossRefGoogle Scholar
  10. 10.
    Dukes BC, Butzke CE (1998) Rapid determination of primary amino acids in grape juice using an o-phthaldialdehyde/N-acetyl-l-cysteine spectrophotometric assay. Am J Enol Vitic 49:125–134Google Scholar
  11. 11.
    Elskens MT, Jasper CJ, Penninckx M (1991) Glutathione as an endogenous sulfur source in the yeast Saccharomyces cerevisiae. J Gen Microbiol 137:637–644CrossRefGoogle Scholar
  12. 12.
    Eschenbruch R, Bonish P, Fisher BM (1978) The production of H2S by pure culture wine yeast. Vitis 17:67–74Google Scholar
  13. 13.
    Giudici P, Kunkee RE (1994) The effect of nitrogen deficiency and sulfur-containing amino acids on the reduction of sulfate to hydrogen sulfide by wine yeasts. Am J Enol Vitic 45:107–112Google Scholar
  14. 14.
    Hallinan P, Saul DJ, Jiranek V (1999) Differential utilisation of sulfur compounds for H2S liberation by nitrogen-starved wine yeasts. Aust J Grape Wine Res 5:82–90CrossRefGoogle Scholar
  15. 15.
    Hernandez-Orte P, Ibarz MJ, Cacho J, Ferreira V (2005) Effect of the addition of ammonium and amino acids to musts of Airen variety on aromatic composition and sensory properties of the obtained wine. Food Chem 89:163–174CrossRefGoogle Scholar
  16. 16.
    Jiranek V, Langridge P, Henschke PA (1991) Yeast nitrogen demand: Selection criterion for wine yeasts for fermenting low nitrogen musts. In: Proceedings of the international symposium on nitrogen in grapes and wine, Seattle, USA. American Society for Enology and Viticulture, Davis, 266–269Google Scholar
  17. 17.
    Jiranek V, Langridge P, Henschke PA (1995) Amino acid and ammonium utilization by Saccharomyces cerevisiae wine yeasts from a chemically defined medium. Am J Enol Vitic 46:75–83Google Scholar
  18. 18.
    Jiranek V, Langridge P, Henschke PA (1995) Regulation of hydrogen sulfide liberation in wine-producing Saccharomyces cerevisiae strains by assimilable nitrogen. Appl Environ Microbiol 61:461–467Google Scholar
  19. 19.
    Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma. S Afr J Enol Vitic 21:97–129Google Scholar
  20. 20.
    Lubbers S, Charpentier C, Feuillat M, Voilley A (1994) Influence of yeasts walls on the behavior of aroma compounds in a model wine. Am J Enol Vitic 45:29–33Google Scholar
  21. 21.
    Lubbers S, Voilley A, Feuillat M, and Charpentier C (1994b) Influence of mannoproteins from yeast on the aroma intensity of a model wine. Lebensmittel – Wissenschaft und Technology 27: 108–114Google Scholar
  22. 22.
    Mendes-Ferreira A, Mendes-Faia A, Lea C (2004) Growth and fermentation patterns of Saccharomyces cerevisiae under different ammonium concentrations and its implications in winemaking industry. J Appl Microbiol 97:540–545CrossRefGoogle Scholar
  23. 23.
    Miller A, Wolff S, Bisson LF, Ebeler S (2007) Yeast strain and nitrogen supplementations: dynamics of volatile ester production in Chardonnay juice fermentations. Am J Enol Vitic 58:470–483Google Scholar
  24. 24.
    Münch P, Hofmann T, Schieberle P (1997) Comparison of key odorants generated by thermal treatment of commercial and self-prepared yeast extracts: influence of the amino acid composition on odorant formation. J Agric Food Chem 45:1338–1344CrossRefGoogle Scholar
  25. 25.
    Münch P, Schieberle P (1998) Quantitative studies on the formation of key odorants in thermally treated yeast extracts using stable isotope dilution assays. J Agric Food Chem 46:4695–4701CrossRefGoogle Scholar
  26. 26.
    Nagodawithana T (1992) Yeast-derived flavors and flavor enhancers and their probable mode of action. Food Technol 46:138–144Google Scholar
  27. 27.
    Ough CS, Amerine MA (1988) Methods for analysis of musts and wines, 2nd edn. Wiley-Interscience, New YorkGoogle Scholar
  28. 28.
    Park SK, Boulton RB, Noble AC (2000) Formation of hydrogen sulfide and glutathione during fermentation of white grape musts. Am J Enol Vitic 51:91–97Google Scholar
  29. 29.
    Penninckx MJ (2002) An overview on glutathione in Saccharomyces versus non-conventional yeasts. FEMS Yeast Res 2:295–305Google Scholar
  30. 30.
    Sablayrolles JM, Dubois C, Manginot C, Roustan JL, Barre P (1996) Effectiveness of combined ammoniacal nitrogen and oxygen additions for completion of sluggish and stuck fermentations. J Ferment Bioeng 82:377–381CrossRefGoogle Scholar
  31. 31.
    Salmon J (1996) Sluggish and stuck fermentations: some actual trends on their physiological basis. Vitic Ecol Sci 51:137–140Google Scholar
  32. 32.
    Sea K, Butzke C, Boulton R (1998) Seasonal variation in the production of hydrogen sulfide during wine fermentations. In: Waterhouse AL, Ebeler SE (eds) Chemistry of wine flavour. American Chemical Society, Washington, pp 81–95CrossRefGoogle Scholar
  33. 33.
    Spiropoulos A, Tanaka J, Flerianos I, Bisson LF (2000) Characterization of hydrogen sulfide formation in commercial and natural wine isolates of Saccharomyces. Am J Enol Vitic 51:233–248Google Scholar
  34. 34.
    Sutherland CM, Henschke PA, Langridge P, De Barros Lopes M (2003) Subunit and cofactor binding of Saccharomyces cerevisiae sulfite reductase—towards developing wine yeast with lowered ability to produce hydrogen sulphide. Aust J Grape Wine Res 9:186–193CrossRefGoogle Scholar
  35. 35.
    Tamayo C, Ubeda J, Briones A (1999) Relationship between H2S-producing strains of wine yeast and different fermentation conditions. Can J Microbiol 45:343–346Google Scholar
  36. 36.
    Torrea D, Varela C, Ugliano M, Ancin-Azpilicueta C, Francis IL, Henschke PA (2011) Comparison of inorganic and organic nitrogen supplementation of grape juice—effect on volatile composition and aroma profile of a Chardonnay wine fermented with Saccharomyces cerevisiae yeast. Food Chem 127:1072–1083CrossRefGoogle Scholar
  37. 37.
    Ugliano M, Fedrizzi B, Siebert T, Travis B, Magno F, Versini G (2009) Effect of nitrogen supplementation and Saccharomyces species on hydrogen sulfide and other volatile sulfur compounds in Shiraz fermentation and wine. J Agric Food Chem 57:4948–4955CrossRefGoogle Scholar
  38. 38.
    Verstrepen KJ, Van Laere SDM, Vanderhaegen BMP, Derdelinckx G, Dufour JP, Pretorius IS et al (2003) Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microb 69:5228–5237CrossRefGoogle Scholar
  39. 39.
    Vilanova M, Ugliano M, Varela C, Siebert T, Pretorius IS, Henschke PA (2007) Assimilable nitrogen utilisation and production of volatile and non-volatile compounds in chemically defined medium by Saccharomyces cerevisiae wine yeasts. Appl Microbiol Biotechnol 77:145–157CrossRefGoogle Scholar
  40. 40.
    Vos PJA, Gray RS (1979) The origin and control of hydrogen sulphide during fermentation of grape must. Am J Enol Vitic 30:187–197Google Scholar
  41. 41.
    Wang XD, Bohlscheid JC, Edwards CG (2003) Fermentative activity and production of volatile compounds by Saccharomyces grown in synthetic grape juice media deficient in assimilable nitrogen and/or pantothenic acid. J Appl Microbiol 94:349–359CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Mirko Gobbi
    • 1
  • Francesca Comitini
    • 1
  • Giuliano D’Ignazi
    • 2
  • Maurizio Ciani
    • 1
  1. 1.Dipartimento Scienze della Vita e dell’AmbienteUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Terre Cortesi Moncaro s.c.r.lMontecarottoItaly

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